Detailed information    

experimental Experimentally validated

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACIAD_RS02540 Genome accession   NC_005966
Coordinates   550472..551272 (+) Length   266 a.a.
NCBI ID   WP_011182074.1    Uniprot ID   A0A7G2SCF1
Organism   Acinetobacter baylyi ADP1     
Function   assists with secretin assembly and localization   
DNA binding and uptake

Function

The competence protein PilF exhibits significant similarities to PilF in P. aeruginosa and N. gonorrhoeae and is suggestd to act as pilotin assisting with secretin complex assembly and localization.


Genomic Context

Location: 545472..556272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIAD_RS02515 (ACIAD0553) - 546397..547686 (+) 1290 WP_011182072.1 glycosyltransferase family 4 protein -
  ACIAD_RS02520 (ACIAD0554) - 547721..548272 (+) 552 WP_026056982.1 phosphatase PAP2 family protein -
  ACIAD_RS02525 (ACIAD0555) iscX 548344..548544 (+) 201 WP_004920012.1 Fe-S cluster assembly protein IscX -
  ACIAD_RS02530 (ACIAD0556) ndk 548655..549086 (+) 432 WP_004920009.1 nucleoside-diphosphate kinase -
  ACIAD_RS02535 (ACIAD0557) rlmN 549209..550453 (+) 1245 WP_004920007.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACIAD_RS02540 (ACIAD0558) pilF 550472..551272 (+) 801 WP_011182074.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIAD_RS02545 (ACIAD0560) - 551263..552045 (+) 783 WP_004919996.1 helix-turn-helix domain-containing protein -
  ACIAD_RS02550 (ACIAD0561) ispG 552069..553184 (+) 1116 WP_004919993.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACIAD_RS02555 (ACIAD0562) hisS 553181..554473 (+) 1293 WP_004919990.1 histidine--tRNA ligase -
  ACIAD_RS02560 (ACIAD0563) - 554505..555206 (+) 702 WP_004919988.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29877.99 Da        Isoelectric Point: 9.5059

>NTDB_id=1017 ACIAD_RS02540 WP_011182074.1 550472..551272(+) (pilF) [Acinetobacter baylyi ADP1]
MSKWTINKTLGCAAIVGAFILSGCQTVPTTKDPEKGVKVRTQLAAEYIKSGDLDSAKRALDQALKVNPKDSSANMMMGVL
LQQEGSRPNLEKADAYFERAVQLDPENAQAHNNYGTYLYQMKRYHDAIQQLTLAGSSLGYDQRYRALENIGRIYLHLGGV
ANAEKMFSQALQANRDSSVSMLELAEIYYLQQRIPAATGMYEQYVRTVGQKNQGARALWIGIRVARANADRTGVQVLVNQ
LRIFYPDSSEYQRYLKLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1017 ACIAD_RS02540 WP_011182074.1 550472..551272(+) (pilF) [Acinetobacter baylyi ADP1]
TTGAGCAAGTGGACAATAAACAAAACGTTGGGTTGTGCCGCCATTGTAGGGGCGTTTATTTTGTCTGGATGTCAGACTGT
TCCAACCACCAAAGACCCTGAAAAGGGTGTAAAAGTACGTACTCAACTTGCTGCCGAATATATTAAATCTGGTGATCTTG
ATTCGGCTAAACGTGCTTTAGATCAAGCCTTAAAAGTAAATCCCAAAGATAGCAGTGCCAACATGATGATGGGTGTGCTG
CTGCAACAAGAAGGTAGTCGCCCTAACCTTGAAAAAGCAGATGCTTATTTTGAGCGAGCTGTACAGCTTGATCCAGAAAA
TGCACAAGCGCATAACAACTATGGTACCTATTTGTATCAGATGAAACGTTATCATGATGCAATTCAACAATTGACACTGG
CTGGTAGTTCATTAGGATATGACCAGCGCTATCGTGCATTGGAAAATATTGGGCGAATTTATTTGCATCTGGGTGGAGTC
GCAAATGCTGAAAAAATGTTCAGTCAGGCGCTTCAGGCAAATCGTGATTCTTCCGTTTCGATGTTAGAGTTAGCAGAGAT
TTATTATTTGCAACAAAGAATTCCTGCCGCAACAGGTATGTACGAACAATATGTGCGTACAGTTGGGCAGAAAAATCAGG
GTGCGCGAGCACTCTGGATTGGTATTCGTGTGGCGAGAGCCAATGCCGATCGGACTGGGGTACAGGTGTTAGTTAATCAG
CTTCGTATTTTTTACCCAGATAGTTCAGAATATCAACGTTATTTGAAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-70)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_16)

(150-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2SCF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.231

97.744

0.726

Multiple sequence alignment    



References

[1] Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
[2] Colleen G Leong et al. (2017) The role of core and accessory type IV pilus genes in natural transformation and twitching motility in the bacterium Acinetobacter baylyi. PloS One 12(8):e0182139. [PMID: 28771515]