Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   A2I97_RS08565 Genome accession   NZ_CP014990
Coordinates   1750827..1751261 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain KD1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1745827..1756261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2I97_RS08560 (A2I97_08315) - 1749327..1750538 (-) 1212 WP_070082710.1 cytochrome P450 family protein -
  A2I97_RS08565 (A2I97_08320) nucA/comI 1750827..1751261 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  A2I97_RS08570 (A2I97_08325) - 1751322..1752077 (+) 756 WP_070081855.1 YoaK family protein -
  A2I97_RS08575 (A2I97_08330) - 1752111..1752473 (-) 363 WP_070081856.1 hypothetical protein -
  A2I97_RS08580 (A2I97_08335) - 1752666..1753994 (-) 1329 WP_070081857.1 S8 family peptidase -
  A2I97_RS08585 (A2I97_08340) - 1754173..1754406 (+) 234 WP_057080789.1 hypothetical protein -
  A2I97_RS08590 (A2I97_08345) - 1754662..1755369 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  A2I97_RS08595 (A2I97_08350) - 1755430..1755882 (+) 453 WP_070081858.1 OsmC family protein -
  A2I97_RS08600 (A2I97_08355) - 1755896..1756249 (-) 354 WP_003154073.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=175772 A2I97_RS08565 WP_007611576.1 1750827..1751261(+) (nucA/comI) [Bacillus velezensis strain KD1]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=175772 A2I97_RS08565 WP_007611576.1 1750827..1751261(+) (nucA/comI) [Bacillus velezensis strain KD1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCAGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment