Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   BFL22_RS23020 Genome accession   NZ_CP018239
Coordinates   4155285..4156409 (-) Length   374 a.a.
NCBI ID   WP_000228545.1    Uniprot ID   -
Organism   Escherichia coli strain 272     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4150285..4161409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL22_RS22990 (BFL22_22715) yrdA 4152085..4152639 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  BFL22_RS22995 (BFL22_22720) yrdB 4152615..4152872 (-) 258 WP_001070590.1 DUF1488 domain-containing protein -
  BFL22_RS23000 (BFL22_22725) aroE 4152869..4153687 (-) 819 WP_000451254.1 shikimate dehydrogenase -
  BFL22_RS23005 (BFL22_22730) tsaC 4153692..4154264 (-) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  BFL22_RS23010 (BFL22_22735) yrdD 4154269..4154811 (-) 543 WP_001129722.1 type I DNA topoisomerase -
  BFL22_RS23015 (BFL22_22740) smg 4154840..4155313 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  BFL22_RS23020 (BFL22_22745) dprA 4155285..4156409 (-) 1125 WP_000228545.1 DNA-protecting protein DprA Machinery gene
  BFL22_RS23025 (BFL22_22750) def 4156539..4157048 (+) 510 WP_000114984.1 peptide deformylase -
  BFL22_RS23030 (BFL22_22755) fmt 4157063..4158010 (+) 948 WP_000004459.1 methionyl-tRNA formyltransferase -
  BFL22_RS23035 (BFL22_22760) rsmB 4158056..4159345 (+) 1290 WP_001301795.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  BFL22_RS23040 (BFL22_22765) trkA 4159367..4160743 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  BFL22_RS23045 (BFL22_22770) mscL 4160873..4161283 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41034.02 Da        Isoelectric Point: 6.3845

>NTDB_id=174247 BFL22_RS23020 WP_000228545.1 4155285..4156409(-) (dprA) [Escherichia coli strain 272]
MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGRPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=174247 BFL22_RS23020 WP_000228545.1 4155285..4156409(-) (dprA) [Escherichia coli strain 272]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCGACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393

Multiple sequence alignment