Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CKU_RS07335 Genome accession   NZ_CP014791
Coordinates   1517206..1517703 (-) Length   165 a.a.
NCBI ID   WP_078255082.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MCRF184     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1512206..1522703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKU_RS07310 (CKU_1378) gcvPB 1512368..1513840 (-) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  CKU_RS07315 (CKU_1379) gcvPA 1513833..1515179 (-) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CKU_RS07320 (CKU_1380) gcvT 1515199..1516290 (-) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  CKU_RS07325 (CKU_1381) - 1516449..1516973 (-) 525 WP_001015120.1 shikimate kinase -
  CKU_RS07330 - 1516963..1517109 (-) 147 WP_001789879.1 hypothetical protein -
  CKU_RS07335 (CKU_1382) comGF 1517206..1517703 (-) 498 WP_078255082.1 competence type IV pilus minor pilin ComGF Machinery gene
  CKU_RS07340 (CKU_1383) comGE 1517621..1517920 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  CKU_RS07345 (CKU_1384) comGD 1517907..1518353 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  CKU_RS07350 (CKU_1385) comGC 1518331..1518642 (-) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  CKU_RS07355 (CKU_1386) comGB 1518656..1519726 (-) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  CKU_RS07360 (CKU_1387) comGA 1519698..1520672 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  CKU_RS07365 (CKU_1388) - 1520724..1521347 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  CKU_RS07370 (CKU_1389) - 1521344..1521673 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CKU_RS07375 (CKU_1390) - 1521673..1522659 (-) 987 WP_000161307.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19168.95 Da        Isoelectric Point: 10.3184

>NTDB_id=174120 CKU_RS07335 WP_078255082.1 1517206..1517703(-) (comGF) [Staphylococcus aureus strain MCRF184]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIKYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=174120 CKU_RS07335 WP_078255082.1 1517206..1517703(-) (comGF) [Staphylococcus aureus strain MCRF184]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCAAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

95.152

100

0.952

Multiple sequence alignment