Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   A0T30_RS18785 Genome accession   NZ_CP014784
Coordinates   4068214..4069806 (+) Length   530 a.a.
NCBI ID   WP_061905388.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain NEB 585     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 4063214..4074806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0T30_RS18765 (A0T30_18765) pgeF 4065009..4065746 (-) 738 WP_061905386.1 peptidoglycan editing factor PgeF -
  A0T30_RS18770 (A0T30_18770) rluD 4065730..4066701 (-) 972 WP_021702235.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  A0T30_RS18775 (A0T30_18775) - 4066849..4067871 (+) 1023 WP_021702236.1 outer membrane protein assembly factor BamD -
  A0T30_RS18780 (A0T30_18780) - 4067993..4068217 (+) 225 WP_061905387.1 PP0621 family protein -
  A0T30_RS18785 (A0T30_18785) pilS 4068214..4069806 (+) 1593 WP_061905388.1 PAS domain-containing sensor histidine kinase Regulator
  A0T30_RS18790 (A0T30_18790) pilR 4069809..4071149 (+) 1341 WP_061905389.1 sigma-54 dependent transcriptional regulator Regulator
  A0T30_RS18795 (A0T30_18795) thiO 4071162..4072262 (-) 1101 WP_061905390.1 glycine oxidase ThiO -
  A0T30_RS18800 (A0T30_18800) - 4072314..4072718 (-) 405 WP_061905391.1 type IV pilin protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59122.44 Da        Isoelectric Point: 7.8529

>NTDB_id=174039 A0T30_RS18785 WP_061905388.1 4068214..4069806(+) (pilS) [Aquipseudomonas alcaligenes strain NEB 585]
MSTEQLTLDGVPGRRILRLYNLYRVIIGLALVLLISSDLDDQLLELAHAQLFRYGSWTYLILNVLVAVLLQRPSHLAQVF
SLALLDVLLLCTLFFAAGGTPSGIGNLMIVAVAIANILLRGRIGLLIAAVAAIGMLALTFYLSLLRNTPAPQFVQAGALG
ALCFAAAIFLQGLASRLQQSEFLAEQRAVDVASLEALNSLILQRMRTGILVLDKQHRVLLANQGALSMLGRSKLEHKLLD
PHCPELVQRLQQWQHNPTLRPQSLQAIADGPLLQPSFIPLQRSGEQHTLIFLDDISQVTQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLEEPDRRLAQIIQDHSRRMNTVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSARPD
QTLHLETQSGSLQTRMDPHQLTQILSNLVQNGLRYSAQKHQLGQVWLKLYRDPESDLPTLEVLDDGPGVPVDQMQHIFEP
FYTTDNKGTGLGLYISRELCESNQARLDYKLREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=174039 A0T30_RS18785 WP_061905388.1 4068214..4069806(+) (pilS) [Aquipseudomonas alcaligenes strain NEB 585]
GTGAGCACCGAGCAGCTGACCCTCGACGGTGTCCCCGGCAGACGCATACTGCGCCTGTACAACCTCTATCGGGTGATCAT
CGGCCTGGCGCTGGTGCTGCTGATTTCCAGCGACCTGGACGACCAGTTACTGGAGCTGGCACACGCCCAGCTGTTCCGCT
ATGGCAGCTGGACCTACCTGATACTCAACGTGTTGGTCGCCGTACTGCTGCAGCGCCCGAGCCACCTGGCCCAGGTCTTC
AGCCTGGCACTGCTCGACGTGCTACTGCTGTGTACCCTGTTCTTCGCCGCAGGCGGCACCCCGAGCGGCATCGGCAACCT
GATGATAGTCGCGGTGGCCATCGCCAACATCCTGCTGCGCGGCCGTATCGGCCTGCTGATCGCCGCCGTCGCCGCCATCG
GCATGCTGGCCCTGACCTTCTATCTCAGCCTGCTGCGCAACACGCCAGCTCCGCAGTTCGTCCAGGCAGGCGCCCTCGGC
GCCCTGTGCTTCGCCGCCGCGATCTTCCTCCAGGGCCTGGCCAGCCGACTGCAGCAGAGCGAGTTCCTGGCCGAACAGCG
TGCCGTCGATGTTGCCAGCCTGGAGGCACTCAACTCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTCCTGGACA
AGCAGCATCGCGTGTTGCTGGCCAATCAGGGCGCCCTCAGCATGCTCGGCCGCAGCAAGCTAGAGCACAAACTGCTCGAC
CCGCACTGCCCGGAGCTGGTCCAGCGCCTGCAGCAGTGGCAGCACAACCCGACCCTGCGCCCGCAGAGCCTGCAGGCCAT
AGCGGACGGCCCGTTGCTGCAGCCGAGCTTCATCCCGCTGCAACGCAGCGGCGAACAACACACCCTGATCTTCCTCGACG
ATATCTCGCAGGTTACCCAGCAGGCCCAGCAGCTCAAGCTGGCCTCCCTGGGTCGCCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTCGGCGCGATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCGGAGGACCTGGAGGAGCCGGATCGGCG
CCTGGCGCAGATCATCCAGGACCATTCGCGGCGAATGAATACGGTGATCGAAAACGTCCTGCAACTGTCGCGCAGGCGTC
AGGCCGAGCCTCAGCTGCTTGACCTGAAATACTGGCTGCACCGCTTCGCCAGCGAGTTCCGCAGTTCCGCACGGCCAGAC
CAGACACTGCACCTGGAGACCCAGAGCGGCAGCCTGCAGACCCGCATGGACCCGCACCAGCTGACGCAGATTCTGAGCAA
TCTGGTGCAAAACGGCCTGCGCTACAGCGCACAGAAGCACCAACTTGGACAGGTCTGGCTCAAGCTCTATCGTGATCCGG
AGAGCGACCTGCCGACACTCGAGGTGCTGGATGACGGCCCCGGCGTACCGGTCGACCAGATGCAGCACATCTTCGAACCC
TTCTACACCACGGACAACAAGGGCACCGGCCTCGGCCTGTATATTTCCCGCGAACTGTGCGAGAGCAATCAAGCACGCCT
CGATTACAAACTGCGCGAGGGTGGCGGCAGTTGCTTCCGCATCACCTTCGCCCACCCACGCAAGCTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

66.038

100

0.66

Multiple sequence alignment