Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AC731_RS16630 Genome accession   NZ_CP014646
Coordinates   3576902..3578362 (-) Length   486 a.a.
NCBI ID   WP_048707789.1    Uniprot ID   A0A127K8Z3
Organism   Thauera humireducens strain SgZ-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3571902..3583362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC731_RS16605 (AC731_016605) - 3572741..3573373 (+) 633 WP_237266555.1 5-formyltetrahydrofolate cyclo-ligase -
  AC731_RS16610 (AC731_016610) ilvA 3573386..3574906 (-) 1521 WP_048707784.1 threonine ammonia-lyase, biosynthetic -
  AC731_RS16615 (AC731_016615) hprK 3575023..3575970 (-) 948 WP_004259797.1 HPr(Ser) kinase/phosphatase -
  AC731_RS16620 (AC731_016620) ptsN 3575939..3576424 (-) 486 WP_048707786.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AC731_RS16625 (AC731_016625) hpf 3576561..3576884 (-) 324 WP_004259801.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AC731_RS16630 (AC731_016630) rpoN/rpoN1 3576902..3578362 (-) 1461 WP_048707789.1 RNA polymerase factor sigma-54 Machinery gene
  AC731_RS16635 (AC731_016635) lptB 3578370..3579092 (-) 723 WP_004259805.1 LPS export ABC transporter ATP-binding protein -
  AC731_RS16640 (AC731_016640) recX 3579079..3579528 (-) 450 WP_048707792.1 recombination regulator RecX -
  AC731_RS16645 (AC731_016645) recA 3579541..3580575 (-) 1035 WP_004259809.1 recombinase RecA Machinery gene
  AC731_RS16650 (AC731_016650) - 3580709..3581194 (-) 486 WP_048707796.1 CinA family protein -
  AC731_RS16655 (AC731_016655) - 3581214..3581696 (-) 483 WP_004259814.1 phosphatidylglycerophosphatase A -
  AC731_RS16660 (AC731_016660) thiL 3581806..3582765 (-) 960 WP_048707797.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 486 a.a.        Molecular weight: 54740.70 Da        Isoelectric Point: 4.8867

>NTDB_id=172978 AC731_RS16630 WP_048707789.1 3576902..3578362(-) (rpoN/rpoN1) [Thauera humireducens strain SgZ-1]
MKPSLQLKLSQHLTLTPQLQQSIKLLQLSTLELNQEIERFLLENPMLEREEIDDFVPQPGLATSTPPAERERERERETSA
EAASDGEAGKANDREDNEEGIDWSNVGSGAQAKRDDDDDTDFQEFQAAELSLRDHLDEQVALSPLSDRDRALVRFIIEAL
DDDGYLHQPLEELLELLPPELDIDLDELSIALRHVQHLDPAGIGARSPQECLSLQLRAMPETPTQMLALEIVDQHLELLA
ERNFARLKKHTGCDDDQLRAAHALICSLDPHPGSRHSVSETRYVLPDVIVRKIRGRWTVSLNQDAMPRLRVNRLYASLLQ
QNRGQGGGLTGQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGDVAMRPLTLREIADQLELHESTVSRVTTQKYM
ATPRGVFELKYFFGSHVSTDTGGAASSTAIRALIRQLVDAEDSKKPLSDARIAEVLGQQGIVVARRTIAKYRESLNIPPV
SLRKSI

Nucleotide


Download         Length: 1461 bp        

>NTDB_id=172978 AC731_RS16630 WP_048707789.1 3576902..3578362(-) (rpoN/rpoN1) [Thauera humireducens strain SgZ-1]
ATGAAACCCAGCCTCCAGCTCAAGCTCTCGCAGCACCTCACGCTGACGCCTCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GCTGTCCACGCTCGAGCTGAACCAGGAGATCGAGCGCTTCCTGCTCGAGAACCCGATGCTCGAGCGCGAGGAGATCGACG
ACTTCGTGCCGCAGCCCGGTCTCGCCACCAGCACCCCGCCTGCCGAACGTGAGCGCGAGCGCGAACGCGAAACCAGCGCC
GAGGCCGCCAGCGACGGCGAAGCAGGAAAGGCGAACGATCGCGAGGACAACGAGGAAGGCATCGACTGGTCCAACGTCGG
CAGTGGCGCCCAGGCCAAGCGCGACGATGACGACGACACCGACTTCCAGGAGTTCCAGGCGGCCGAGCTGTCGCTTCGCG
ACCACCTCGACGAACAGGTGGCGCTGTCGCCGCTCTCCGACCGCGACCGCGCGCTGGTGCGCTTCATCATCGAGGCGCTG
GACGACGACGGCTACTTGCACCAGCCGCTCGAGGAATTGCTCGAGTTGCTACCACCCGAGCTCGACATCGACCTCGACGA
GCTTTCCATCGCGCTGCGCCACGTGCAGCACCTCGACCCTGCCGGCATCGGCGCACGCAGCCCGCAGGAATGCCTGTCGC
TCCAGTTGCGTGCGATGCCCGAGACGCCGACGCAGATGCTCGCGCTCGAGATCGTTGACCAGCATCTCGAACTGCTCGCC
GAGCGCAACTTCGCGCGCCTCAAGAAACACACCGGTTGCGACGACGACCAGCTGCGCGCTGCCCACGCTCTGATCTGCAG
CCTCGACCCGCATCCGGGCTCGCGCCACTCCGTCTCCGAGACGCGCTACGTGCTGCCTGACGTCATCGTGCGCAAGATCC
GCGGGCGCTGGACCGTCAGCCTCAACCAGGACGCGATGCCGCGCCTGCGCGTCAACCGGCTCTACGCCAGCCTGCTGCAG
CAGAACCGCGGCCAGGGCGGCGGCCTGACCGGCCAGTTACAGGAAGCACGCTGGCTGATCAAGAACGTCCAGCAGCGTTT
CGACACCATCCTGCGGGTCTCCCAGGCCATCGTTGACCAGCAGCGGCAGTTCTTCGATCATGGAGACGTGGCGATGCGGC
CACTCACTCTCCGGGAGATCGCGGACCAGCTCGAACTGCACGAATCCACCGTTTCGCGCGTCACAACGCAGAAATACATG
GCGACGCCGCGCGGTGTGTTCGAACTCAAGTATTTCTTCGGCAGCCACGTCTCCACCGACACTGGCGGAGCGGCCTCTTC
CACGGCGATTCGGGCACTGATTCGTCAACTGGTGGACGCCGAGGACAGCAAGAAGCCGCTGTCCGACGCACGGATTGCAG
AAGTTTTGGGCCAGCAGGGTATCGTGGTCGCACGACGCACCATCGCGAAGTACCGCGAATCACTGAATATCCCGCCGGTC
AGTCTGCGCAAATCGATCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-484)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(122-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.084

100

0.599

Multiple sequence alignment