Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   AR327_RS11230 Genome accession   NZ_CP014643
Coordinates   2308327..2308659 (+) Length   110 a.a.
NCBI ID   WP_012295159.1    Uniprot ID   -
Organism   Lysinibacillus sphaericus strain OT4b.25     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2303327..2313659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AR327_RS11200 (AR327_11200) - 2303799..2303978 (-) 180 WP_031416957.1 DUF2759 domain-containing protein -
  AR327_RS11205 (AR327_11205) - 2304147..2304791 (+) 645 WP_012295163.1 MBL fold metallo-hydrolase -
  AR327_RS11210 (AR327_11210) - 2304851..2305093 (-) 243 WP_004231035.1 DUF2626 family protein -
  AR327_RS11215 (AR327_11215) - 2305211..2305921 (-) 711 WP_012295162.1 helix-turn-helix transcriptional regulator -
  AR327_RS11220 (AR327_11220) comGA 2306250..2307269 (+) 1020 WP_012295161.1 competence type IV pilus ATPase ComGA -
  AR327_RS25065 - 2307244..2307465 (+) 222 WP_235616146.1 hypothetical protein -
  AR327_RS11225 (AR327_11225) comGB 2307474..2308316 (+) 843 WP_235616147.1 competence type IV pilus assembly protein ComGB -
  AR327_RS11230 (AR327_11230) comGC 2308327..2308659 (+) 333 WP_012295159.1 competence type IV pilus major pilin ComGC Machinery gene
  AR327_RS11235 (AR327_11235) comGD 2308659..2309099 (+) 441 WP_012295158.1 competence type IV pilus minor pilin ComGD -
  AR327_RS11240 (AR327_11240) - 2309092..2309409 (+) 318 WP_012295157.1 hypothetical protein -
  AR327_RS11245 (AR327_11245) comGF 2309402..2309830 (+) 429 WP_012295156.1 competence type IV pilus minor pilin ComGF -
  AR327_RS11255 (AR327_11255) - 2310029..2310544 (+) 516 WP_031416947.1 shikimate kinase -
  AR327_RS11260 (AR327_11260) gcvT 2311047..2312150 (+) 1104 WP_012295154.1 glycine cleavage system aminomethyltransferase GcvT -
  AR327_RS11265 (AR327_11265) gcvPA 2312176..2313522 (+) 1347 WP_012295153.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 110 a.a.        Molecular weight: 12302.40 Da        Isoelectric Point: 4.6258

>NTDB_id=172944 AR327_RS11230 WP_012295159.1 2308327..2308659(+) (comGC) [Lysinibacillus sphaericus strain OT4b.25]
MKQIKQQNGFTLIEMLIVLLIISILILITIPNVTKHFATIDEKGCAAYINMVQGQVEAYRVDFMTFPTLEDLVKEGYLKE
NETTCPNKEEIVITIKGEVRLANADAGTQG

Nucleotide


Download         Length: 333 bp        

>NTDB_id=172944 AR327_RS11230 WP_012295159.1 2308327..2308659(+) (comGC) [Lysinibacillus sphaericus strain OT4b.25]
ATGAAACAAATCAAACAACAAAATGGCTTTACTTTAATCGAAATGCTGATTGTTTTGTTAATTATATCAATCCTTATTTT
AATTACAATTCCAAATGTTACGAAGCACTTTGCTACGATTGATGAGAAGGGCTGTGCGGCCTATATTAATATGGTGCAGG
GGCAAGTAGAGGCCTATAGAGTGGATTTTATGACATTTCCGACCCTTGAGGATTTAGTGAAGGAGGGCTATTTGAAAGAA
AATGAAACAACTTGCCCTAATAAAGAAGAAATAGTCATTACGATTAAAGGAGAGGTACGCTTAGCAAATGCTGATGCAGG
TACTCAAGGATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

54.082

89.091

0.482
  comGC Staphylococcus aureus MW2

45.263

86.364

0.391
  comGC Staphylococcus aureus N315

45.263

86.364

0.391

Multiple sequence alignment