Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   BAMY_RS08545 Genome accession   NZ_CP017953
Coordinates   1667375..1669960 (+) Length   861 a.a.
NCBI ID   WP_003154108.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Y14     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1662375..1674960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BAMY_RS08525 (BAMY_08330) - 1663174..1664352 (+) 1179 WP_095324086.1 glycine C-acetyltransferase -
  BAMY_RS08530 (BAMY_08335) miaB 1664495..1666024 (+) 1530 WP_003154116.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  BAMY_RS08535 (BAMY_08340) ymcA 1666027..1666458 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  BAMY_RS08540 (BAMY_08345) cotE 1666711..1667256 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  BAMY_RS08545 (BAMY_08350) hexA 1667375..1669960 (+) 2586 WP_003154108.1 DNA mismatch repair protein MutS Machinery gene
  BAMY_RS08550 (BAMY_08355) hexB 1669976..1671853 (+) 1878 WP_003154106.1 DNA mismatch repair endonuclease MutL Machinery gene
  BAMY_RS08555 (BAMY_08360) - 1672174..1672383 (-) 210 WP_003154104.1 hypothetical protein -
  BAMY_RS08560 (BAMY_08365) - 1673454..1674131 (+) 678 WP_003154103.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97700.96 Da        Isoelectric Point: 5.3594

>NTDB_id=172133 BAMY_RS08545 WP_003154108.1 1667375..1669960(+) (hexA) [Bacillus amyloliquefaciens strain Y14]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRNGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=172133 BAMY_RS08545 WP_003154108.1 1667375..1669960(+) (hexA) [Bacillus amyloliquefaciens strain Y14]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCGCAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACCCCGGGAACCGTGATGGATGGAAAAGGCATCCATGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTAACGGATACGGTCTCGCTTTATCAGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACGGCTGCTCAGCTGAA
AGAACGGTGCGGCGCAACGATTTCTATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCATCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCAATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGCGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCAGGGTCGCATTCGGAAACGT
CAACGCAAGAGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATTAAAGAGCTTGTAGGATCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGGGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAACTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGATTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGTCTTCGTGAAAAGGTGAAACAATTTATTCCGCGTCTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGATGCGCTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGTGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAAATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCAATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTTCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCGGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCGGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

MutS domain I (MutS_I)

(5-116)

MutS domain II (MutS_II)

(125-239)

MutS domain V (MutS_V)

(598-785)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain III (MutS_III)

(258-547)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

48.191

99.535

0.48
  mutS Pseudomonas stutzeri strain ATCC 17587

38.549

100

0.395

Multiple sequence alignment