Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   EfmE745_RS03395 Genome accession   NZ_CP014529
Coordinates   712340..714022 (+) Length   560 a.a.
NCBI ID   WP_002286651.1    Uniprot ID   A0A132P3G9
Organism   Enterococcus faecium strain E745     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 707340..719022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EfmE745_RS03365 (EfmE745_00697) - 707626..708480 (+) 855 WP_002286660.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
  EfmE745_RS03370 (EfmE745_00698) xseA 708481..709818 (+) 1338 WP_002286658.1 exodeoxyribonuclease VII large subunit -
  EfmE745_RS03375 (EfmE745_00699) - 709823..710047 (+) 225 WP_002286657.1 exodeoxyribonuclease VII small subunit -
  EfmE745_RS03380 (EfmE745_00700) - 710049..710939 (+) 891 WP_002286656.1 polyprenyl synthetase family protein -
  EfmE745_RS03385 (EfmE745_00701) - 710948..711766 (+) 819 WP_002286655.1 TlyA family RNA methyltransferase -
  EfmE745_RS03390 (EfmE745_00702) - 711873..712322 (+) 450 WP_002286653.1 arginine repressor -
  EfmE745_RS03395 (EfmE745_00703) recN 712340..714022 (+) 1683 WP_002286651.1 DNA repair protein RecN Machinery gene
  EfmE745_RS03400 (EfmE745_00704) - 714077..714193 (-) 117 WP_002291876.1 DUF4044 domain-containing protein -
  EfmE745_RS03405 (EfmE745_00705) - 714266..715222 (-) 957 WP_002286649.1 magnesium transporter CorA family protein -
  EfmE745_RS03410 (EfmE745_00706) - 715393..715761 (+) 369 WP_002286647.1 DUF3397 domain-containing protein -
  EfmE745_RS03415 (EfmE745_00707) mraZ 715931..716362 (+) 432 WP_002286645.1 division/cell wall cluster transcriptional repressor MraZ -
  EfmE745_RS03420 (EfmE745_00708) rsmH 716383..717342 (+) 960 WP_002286642.1 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH -
  EfmE745_RS03425 (EfmE745_00709) ftsL 717347..717748 (+) 402 WP_002294542.1 cell division protein FtsL -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 63377.82 Da        Isoelectric Point: 4.6049

>NTDB_id=172133 EfmE745_RS03395 WP_002286651.1 712340..714022(+) (recN) [Enterococcus faecium strain E745]
MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGLLAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELM
VELGIETDEDNLIVRRDMSLTGKNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEEREKLSNYQKIADGLAAGYGALTNN
EQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAYYLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGES
IESILAYYDEITEELASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMENTEFEVRFL
KEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIFSSEQGVTSIIFDEVDTGVSGRVAQAIADKI
SQISKYSQVLCITHLPQVAAVADYQYYIVKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=172133 EfmE745_RS03395 WP_002286651.1 712340..714022(+) (recN) [Enterococcus faecium strain E745]
ATGCTACAAGAAATCAGTATTACTAACTTTGCGATTATTCCGGAATTGCGGCTTTCTTTTCACGAAGGAATGACCGCGCT
GACCGGAGAGACTGGTGCGGGTAAATCCATTATTATCGATGCTTTAGGTCTGTTAGCAGGAGGAAGAGGTTCAAGCGATT
ATATACGTCAAGGAGCAGAAAAATGTGTGCTTGAAGGGTTGTTTGAACTACCAAAACAAGAAGGATTCTCTGAACTGATG
GTTGAATTAGGGATCGAAACAGATGAGGACAATCTGATTGTTCGACGGGATATGTCCTTAACTGGAAAAAATGTTTGTCG
AGTGAATGGTCATATTATCACATTAGCTAATCTGAGAAGAATCGGCAGTTATTTAGTAGATATCCAAGGACAAAACGAAC
ATCAGGAACTGCTCCAGCCTGAATCACATCTTGCTTTATTGGATCGTTTTGGAGATGTAGCTTTCCAACAGAAGAAAAAG
AGTTATCAGCAAGAATATATCTCTTATCGTGAGCTGGAAAAAAGGGTACGAAAAGTGCAGCAAAATGAAAAATCCTATGT
TCAGAGAATAGATATGCTCCATTTCCAGCAAGAAGAAATAGCTGCTGCTGATTTGCAAGTCGGAGAAGAAGAGAAATTAA
AAGAAGAAAGAGAAAAACTTAGCAATTATCAAAAAATTGCTGATGGATTAGCTGCAGGTTACGGAGCACTGACGAATAAT
GAACAAAACAGTGTAGATGGTGTTGGCTTGGCCGTTTCAGAACTGCAAGGGATCGCCCATCTAGATGTGGAGTATGAAGC
AATTTATGAAAATATCCAAAGTGCTTACTATTTGCTGCAAGATGCAATCGGCGATATGAGTCGTCAGATTGATTTATTGG
AGTTAGATGAGAGTCGTTTAGAAGAAGTTACGCAACGTTTAGAATTAATCCGACAGTTGAAACGTAAATACGGCGAATCC
ATTGAGTCAATATTGGCTTACTATGACGAAATCACCGAAGAGCTAGCTTCTTCTGATTTTTCAGAAGGTCAATTGGACAA
AATGAAAGAGGAACTAGAGCAAAAAGAGCTTCTACTTCAGCAACAAGCCGCAGATCTTCATGAAGCGCGTAAAAAAATCG
CAAAAGAATTGGAAAAATCCATTCTGCATGAATTGAAAAGTCTGTATATGGAAAACACTGAGTTTGAGGTACGTTTCTTA
AAAGAAGAAAATAGACAATTGAATCATGATGGATTTGACCAAATCGAATTTTACATTACGACCAATCCAGGAGAGCCACT
GAAGCCTTTGGTGAAAGTCGCTTCTGGTGGCGAACTGTCTCGGATGTTGCTTGCTTTGAAAACTATTTTTTCTTCTGAGC
AAGGTGTGACAAGTATTATTTTTGATGAAGTGGATACAGGTGTGAGCGGAAGAGTTGCACAGGCCATCGCAGATAAAATT
TCTCAAATATCGAAATACTCTCAAGTTTTGTGCATCACTCATCTGCCTCAAGTTGCTGCAGTCGCCGACTATCAATATTA
TATTGTGAAAGCAGTGATTGATGGACGTACGCAAACTTCTGTTTCAGAGTTGAAAACAAAAGAAAGAGAAGAAGAAATTG
CACGTATGCTGGCTGGAAGTGAGATTACAAAACTCACACTCGAACATGCAAAAGAACTATTGAAACTCGCAAAAAGGACC
TGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(1-510)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A132P3G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.5

Multiple sequence alignment