Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   AWV81_RS08945 Genome accession   NZ_CP014471
Coordinates   1693044..1693937 (+) Length   297 a.a.
NCBI ID   WP_014479775.1    Uniprot ID   A0AA96ZWR3
Organism   Bacillus subtilis subsp. natto strain CGMCC 2108     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1688044..1698937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWV81_RS08925 (AWV81_08920) - 1688714..1690444 (+) 1731 WP_014479774.1 hypothetical protein -
  AWV81_RS08930 (AWV81_08925) ylqH 1690441..1690722 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  AWV81_RS08935 (AWV81_08930) sucC 1690895..1692052 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  AWV81_RS08940 (AWV81_08935) sucD 1692081..1692983 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  AWV81_RS08945 (AWV81_08940) dprA 1693044..1693937 (+) 894 WP_014479775.1 DNA-processing protein DprA Machinery gene
  AWV81_RS08950 (AWV81_08945) topA 1694125..1696200 (+) 2076 WP_014479776.1 type I DNA topoisomerase -
  AWV81_RS08955 (AWV81_08950) trmFO 1696276..1697583 (+) 1308 WP_003244725.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  AWV81_RS08960 (AWV81_08955) xerC 1697651..1698565 (+) 915 WP_014479777.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32907.21 Da        Isoelectric Point: 9.9142

>NTDB_id=171719 AWV81_RS08945 WP_014479775.1 1693044..1693937(+) (dprA) [Bacillus subtilis subsp. natto strain CGMCC 2108]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQFYPKLPRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=171719 AWV81_RS08945 WP_014479775.1 1693044..1693937(+) (dprA) [Bacillus subtilis subsp. natto strain CGMCC 2108]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCTGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAATTTTATCCAAAGCTCCCGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCTGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACGAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

99.663

100

0.997
  dprA Legionella pneumophila strain ERS1305867

38.462

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377

Multiple sequence alignment