Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AYM14_RS08385 Genome accession   NZ_CP014365
Coordinates   1642919..1643416 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain USA300-SUR2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1637919..1648416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYM14_RS08360 (AYM14_08150) gcvPB 1638081..1639553 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  AYM14_RS08365 (AYM14_08155) gcvPA 1639546..1640892 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AYM14_RS08370 (AYM14_08160) gcvT 1640912..1642003 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AYM14_RS08375 (AYM14_08165) - 1642162..1642686 (-) 525 WP_001015117.1 shikimate kinase -
  AYM14_RS08380 (AYM14_08170) - 1642676..1642822 (-) 147 WP_001789879.1 hypothetical protein -
  AYM14_RS08385 (AYM14_08175) comGF 1642919..1643416 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  AYM14_RS08390 (AYM14_08180) comGE 1643334..1643633 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AYM14_RS08395 (AYM14_08185) comGD 1643620..1644066 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  AYM14_RS08400 (AYM14_08190) comGC 1644044..1644355 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AYM14_RS08405 (AYM14_08195) comGB 1644369..1645439 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  AYM14_RS08410 (AYM14_08200) comGA 1645411..1646385 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  AYM14_RS08415 (AYM14_08205) - 1646437..1647060 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AYM14_RS08420 (AYM14_08210) - 1647057..1647386 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AYM14_RS08425 (AYM14_08215) - 1647386..1648372 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=170730 AYM14_RS08385 WP_001789864.1 1642919..1643416(-) (comGF) [Staphylococcus aureus strain USA300-SUR2]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=170730 AYM14_RS08385 WP_001789864.1 1642919..1643416(-) (comGF) [Staphylococcus aureus strain USA300-SUR2]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment