Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   AXW78_RS21040 Genome accession   NZ_CP014282
Coordinates   4207536..4209875 (-) Length   779 a.a.
NCBI ID   WP_061884595.1    Uniprot ID   A0A9W3YIL8
Organism   Bacillus thuringiensis strain Bt185     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4202536..4214875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW78_RS34480 (AXW78_21015) - 4203418..4203594 (-) 177 WP_001242605.1 hypothetical protein -
  AXW78_RS21020 (AXW78_21020) - 4203677..4204009 (-) 333 WP_000938749.1 YrdB family protein -
  AXW78_RS21025 (AXW78_21025) - 4204125..4204565 (-) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  AXW78_RS21030 (AXW78_21030) relA 4204582..4206765 (-) 2184 WP_001262803.1 GTP diphosphokinase -
  AXW78_RS21035 (AXW78_21035) - 4206976..4207488 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  AXW78_RS21040 (AXW78_21040) recJ 4207536..4209875 (-) 2340 WP_061884595.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  AXW78_RS21045 (AXW78_21045) - 4209977..4210870 (-) 894 WP_000409743.1 cation diffusion facilitator family transporter -
  AXW78_RS21050 (AXW78_21050) secDF 4211027..4213291 (-) 2265 WP_001119051.1 protein translocase subunit SecDF -
  AXW78_RS21055 (AXW78_21055) - 4213561..4213845 (-) 285 WP_000886980.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88189.75 Da        Isoelectric Point: 4.9368

>NTDB_id=170204 AXW78_RS21040 WP_061884595.1 4207536..4209875(-) (recJ) [Bacillus thuringiensis strain Bt185]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAKVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKVGFPERIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=170204 AXW78_RS21040 WP_061884595.1 4207536..4209875(-) (recJ) [Bacillus thuringiensis strain Bt185]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAAAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACGGTCGACACGGGCATCGCAGCAGTACATGAAGCGAATGTAGCGAAGGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGAAGGTGGCGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCATTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGTGGTTTAAAACATATGCGTATGACGAGAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAGCGC
CGGCTGTACACCTTTTATTATCAGATGATCCAGAGGAAGCGAAGGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTTGAAAATAACTTCCCGCCAGAAGAAAACAAAGT
ATTAGTACTTGCAAAAGAAGGCTGGAACCCAGGGGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTACCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGCTTAAATGAACAAGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGTAAAGTAGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAAAAACTAGCTCCATTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTAAAAGATGCAGAGTTAGAAAGCATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCGTCCATTAATGAATGGAATAATTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGTGAG
CTATTTAAGGTAGGATTCCCAGAGCGAATTTATACGCTATTTTATCAAGAGAATAACCATTTATTTAGTACAGTTCCGAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.455

99.358

0.521

Multiple sequence alignment