Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AWU82_RS26275 Genome accession   NZ_CP014205
Coordinates   5759747..5761336 (+) Length   529 a.a.
NCBI ID   WP_064382094.1    Uniprot ID   -
Organism   Pseudomonas glycinae strain MS586     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5754747..5766336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWU82_RS26255 (AWU82_16325) pgeF 5756548..5757270 (-) 723 WP_064382093.1 peptidoglycan editing factor PgeF -
  AWU82_RS26260 (AWU82_16330) rluD 5757267..5758229 (-) 963 WP_011335991.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AWU82_RS26265 (AWU82_16335) - 5758382..5759398 (+) 1017 WP_007959566.1 outer membrane protein assembly factor BamD -
  AWU82_RS26270 (AWU82_16340) - 5759524..5759757 (+) 234 WP_011335992.1 PP0621 family protein -
  AWU82_RS26275 (AWU82_16345) pilS 5759747..5761336 (+) 1590 WP_064382094.1 sensor histidine kinase Regulator
  AWU82_RS26280 (AWU82_16350) pilR 5761333..5762679 (+) 1347 WP_064382095.1 sigma-54-dependent transcriptional regulator Regulator
  AWU82_RS26285 (AWU82_16355) thiO 5762822..5763922 (-) 1101 WP_064382096.1 glycine oxidase ThiO -
  AWU82_RS26290 (AWU82_16360) - 5763980..5764381 (-) 402 WP_064382097.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58593.33 Da        Isoelectric Point: 6.6061

>NTDB_id=169394 AWU82_RS26275 WP_064382094.1 5759747..5761336(+) (pilS) [Pseudomonas glycinae strain MS586]
MIAEADNADSKQAQRLLRLYHLYRLSVGITLVLLISSNMDNRLLTSANDEWLRNGSWLYLVLNILLVVFLENIHRPAQLF
ALALVDILLLCGVFYAAGGVASAIGNLLIVSVAISNTLLRRRIGLLIAAIATLGIVGLSFVLSFSHPLSANDYLQAGTLG
ALCFAASLLVQGLIRRLEVSENLAELRASEVVGLEALNALILQRMRTGILVLDEQRRVQLANHSAKALLGQTHLEGDLID
EHSSALVERLQLWLNNPTLRPQSLKIAGNGLELQPSFIALEQSPNRQTLVFLEDLAQIAQQAQQLKLAALGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDSADRRLTQIIQDHSQRMNRVIENVLQLSRRQQSAPQRLDLKPWLEQFVSESREQAGER
QHIHLHIGPGDFRTLMDPGQLTQILDNLLRNGWRHSALLHDPAEVWLTLFIDPESTLAVLEVQDNGPGVSLEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKSRQGGGCFRITFAHGRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=169394 AWU82_RS26275 WP_064382094.1 5759747..5761336(+) (pilS) [Pseudomonas glycinae strain MS586]
GTGATCGCTGAGGCCGACAACGCCGACAGCAAACAGGCCCAGCGACTGCTGCGCCTCTATCATCTGTACCGTTTAAGTGT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAACCGCCTGCTGACCTCGGCCAACGACGAGTGGCTGCGCA
ATGGCAGCTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTCCTGGAGAACATCCACCGCCCGGCCCAGCTGTTC
GCTCTGGCACTGGTCGACATCCTGCTGCTGTGCGGCGTGTTCTATGCGGCCGGCGGTGTGGCCAGTGCGATTGGCAACTT
GCTGATTGTTTCGGTGGCTATCAGCAACACGTTGCTGCGACGACGCATCGGCTTGCTGATCGCCGCCATCGCCACCCTCG
GCATCGTCGGGCTCAGCTTTGTGCTGAGTTTCAGCCACCCCTTGAGCGCCAACGACTATCTGCAGGCCGGCACCCTCGGC
GCCTTGTGCTTCGCCGCCTCGCTGCTGGTGCAAGGGTTGATCCGCCGTCTTGAAGTCAGTGAGAACCTGGCCGAGCTGCG
CGCCAGCGAGGTGGTGGGGCTCGAAGCCCTCAATGCGTTGATCCTGCAACGCATGCGCACCGGCATTCTGGTGCTCGACG
AACAGCGCCGGGTGCAATTGGCCAACCACAGCGCCAAGGCCCTGCTGGGACAAACGCACCTTGAGGGTGACTTGATCGAT
GAGCACTCGAGCGCACTGGTCGAACGCCTGCAGCTGTGGCTGAACAACCCGACCCTGCGCCCGCAAAGCCTGAAAATCGC
CGGCAACGGCCTGGAGCTGCAACCAAGCTTCATCGCCCTGGAACAAAGTCCGAACCGTCAAACCCTGGTGTTTCTCGAAG
ACCTCGCCCAGATCGCCCAACAGGCCCAGCAATTGAAACTTGCTGCGCTGGGCCGACTGACCGCCGGTATCGCCCATGAA
ATCCGCAATCCGCTGGGGGCCATCAGTCATGCTGCACAGCTGTTGCAGGAGTCCGAGGAACTCGACAGCGCGGATCGGCG
TCTGACGCAGATCATTCAAGACCACTCCCAGCGCATGAACCGAGTCATCGAAAACGTCCTGCAACTGTCCCGCCGTCAGC
AGAGCGCGCCGCAACGGCTGGATCTCAAACCGTGGCTGGAGCAGTTCGTCAGCGAGAGCCGCGAACAGGCTGGCGAGCGC
CAGCATATTCACCTGCACATCGGCCCGGGGGATTTTCGCACCCTGATGGATCCCGGCCAGCTCACGCAGATTCTCGACAA
TCTGTTACGCAATGGCTGGCGCCACAGTGCCCTGCTACACGATCCGGCCGAGGTGTGGCTGACGTTGTTCATTGACCCCG
AGAGCACGCTGGCCGTGCTCGAAGTGCAGGACAACGGCCCCGGTGTGTCGCTGGAACAACAGGCTCATCTGTTCGAACCT
TTCTTTACCACCAGCAGCCAGGGCACCGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAAGCGCGCCT
AGACTTCAAATCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAAAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-521)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.811

100

0.599

Multiple sequence alignment