Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   AWM80_RS08765 Genome accession   NZ_CP014166
Coordinates   1661846..1662739 (+) Length   297 a.a.
NCBI ID   WP_003245871.1    Uniprot ID   A0AAE2SJU2
Organism   Bacillus subtilis subsp. subtilis strain CU1050     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1656846..1667739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM80_RS08745 (AWM80_08750) - 1657516..1659246 (+) 1731 WP_003245096.1 hypothetical protein -
  AWM80_RS08750 (AWM80_08755) ylqH 1659243..1659524 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  AWM80_RS08755 (AWM80_08760) sucC 1659697..1660854 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  AWM80_RS08760 (AWM80_08765) sucD 1660883..1661785 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  AWM80_RS08765 (AWM80_08770) dprA 1661846..1662739 (+) 894 WP_003245871.1 DNA-processing protein DprA Machinery gene
  AWM80_RS08770 (AWM80_08775) topA 1662927..1665002 (+) 2076 WP_003245599.1 type I DNA topoisomerase -
  AWM80_RS08775 (AWM80_08780) trmFO 1665078..1666385 (+) 1308 WP_003244725.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  AWM80_RS08780 (AWM80_08785) xerC 1666453..1667367 (+) 915 WP_003231988.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32935.26 Da        Isoelectric Point: 9.9142

>NTDB_id=169121 AWM80_RS08765 WP_003245871.1 1661846..1662739(+) (dprA) [Bacillus subtilis subsp. subtilis strain CU1050]
MDQAAVCLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQFYPKLPRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=169121 AWM80_RS08765 WP_003245871.1 1661846..1662739(+) (dprA) [Bacillus subtilis subsp. subtilis strain CU1050]
TTGGATCAGGCCGCTGTCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAATTTTATCCAAAGCTCCCGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCTGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGCACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  dprA Legionella pneumophila strain ERS1305867

38.462

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377

Multiple sequence alignment