Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   BHW76_RS17400 Genome accession   NZ_CP017249
Coordinates   3316050..3317060 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain NADC 5570/86-24/6565 isolate mutant     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3311050..3322060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHW76_RS17380 (BHW76_17380) mepM 3311918..3313240 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  BHW76_RS17385 (BHW76_17385) znuA 3313256..3314188 (-) 933 WP_001303192.1 zinc ABC transporter substrate-binding protein ZnuA -
  BHW76_RS17390 (BHW76_17390) znuC 3314267..3315022 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  BHW76_RS17395 (BHW76_17395) znuB 3315019..3315804 (+) 786 WP_000571476.1 zinc ABC transporter permease subunit ZnuB -
  BHW76_RS17400 (BHW76_17400) ruvB 3316050..3317060 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  BHW76_RS17405 (BHW76_17405) ruvA 3317069..3317680 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  BHW76_RS30015 yobI 3317819..3317884 (-) 66 WP_010723105.1 stress response small protein YobI -
  BHW76_RS17410 (BHW76_17410) yebB 3317955..3318557 (+) 603 WP_001024949.1 YebB family permuted papain-like enzyme -
  BHW76_RS17415 (BHW76_17415) ruvC 3318559..3319080 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  BHW76_RS17420 (BHW76_17420) yebC 3319115..3319855 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  BHW76_RS17425 (BHW76_17425) nudB 3319884..3320336 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=167334 BHW76_RS17400 WP_000568519.1 3316050..3317060(-) (ruvB) [Escherichia coli strain NADC 5570/86-24/6565 isolate mutant]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=167334 BHW76_RS17400 WP_000568519.1 3316050..3317060(-) (ruvB) [Escherichia coli strain NADC 5570/86-24/6565 isolate mutant]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment