Detailed information NTG0166473

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: NEB5A_RS15460
• Genome accession: NZ_CP017100
• Coordinates: 3110374..3111033 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221495.1
• Uniprot ID: P52076
• Organism: Escherichia coli strain K-12 NEB 5-alpha
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3105374..3116033

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NEB5A_RS15435 (NEB5A_15435)	ygiS	3106657..3108264 (-)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
NEB5A_RS15440 (NEB5A_15440)	mqsA	3108397..3108792 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
NEB5A_RS15445 (NEB5A_15445)	mqsR	3108794..3109090 (-)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
NEB5A_RS15450 (NEB5A_15450)	ygiV	3109295..3109777 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
NEB5A_RS15455 (NEB5A_15455)	ygiW	3109830..3110222 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
NEB5A_RS15460 (NEB5A_15460)	ciaR	3110374..3111033 (+)	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	Regulator
NEB5A_RS15465 (NEB5A_15465)	qseC	3111030..3112379 (+)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
NEB5A_RS15470 (NEB5A_15470)	ygiZ	3112425..3112757 (-)	333	WP_000917684.1	DUF2645 family protein	-
NEB5A_RS15475 (NEB5A_15475)	mdaB	3113076..3113657 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
NEB5A_RS15480 (NEB5A_15480)	ygiN	3113688..3114002 (+)	315	WP_000958598.1	putative quinol monooxygenase	-
NEB5A_RS15485 (NEB5A_15485)	parE	3114050..3115942 (-)	1893	WP_000195296.1	DNA topoisomerase IV subunit B	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24677.59 Da
• Isoelectric Point: 6.9850

>NTDB_id=166473 NEB5A_RS15460 WP_001221495.1 3110374..3111033(+) (ciaR) [Escherichia coli strain K-12 NEB 5-alpha]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=166473 NEB5A_RS15460 WP_001221495.1 3110374..3111033(+) (ciaR) [Escherichia coli strain K-12 NEB 5-alpha]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Response regulator receiver domain (Response_reg) (3-112)

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

3D structure

• AlphaFold DB: P52076

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	37.885	100	0.393
ciaR	Streptococcus pneumoniae D39	37.885	100	0.393
ciaR	Streptococcus pneumoniae R6	37.885	100	0.393
ciaR	Streptococcus pneumoniae TIGR4	37.885	100	0.393
ciaR	Streptococcus mutans UA159	35.426	100	0.361