Detailed information NTG0166314

Bioinformatically predicted

Overview

• Name: comFA/cflA
• Type: Machinery gene
• Locus tag: AVT04_RS03055
• Genome accession: NZ_CP013939
• Coordinates: 580932..582251 (-)
• Length: 439 a.a.
• NCBI ID: WP_011226886.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus strain S9
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	569450..580951	580932..582251	flank	-19

Gene organization within MGE regions

Location: 569450..582251

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AVT04_RS02970 (AVT04_02970)	recX	569450..570226 (+)	777	WP_002949763.1	recombination regulator RecX	-
AVT04_RS02975 (AVT04_02975)	-	570297..570830 (+)	534	WP_002949762.1	DUF402 domain-containing protein	-
AVT04_RS02980 (AVT04_02980)	-	570860..571519 (-)	660	WP_011225520.1	DUF1803 domain-containing protein	-
AVT04_RS02985 (AVT04_02985)	-	571573..572505 (-)	933	WP_011225519.1	manganese-dependent inorganic pyrophosphatase	-
AVT04_RS03040 (AVT04_03040)	-	578679..579632 (+)	954	WP_041826943.1	IS30 family transposase	-
AVT04_RS03045 (AVT04_03045)	hpf	579662..580210 (-)	549	WP_011225518.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
AVT04_RS03050 (AVT04_03050)	-	580289..580951 (-)	663	WP_002949747.1	ComF family protein	-
AVT04_RS03055 (AVT04_03055)	comFA/cflA	580932..582251 (-)	1320	WP_011226886.1	DEAD/DEAH box helicase	Machinery gene

Sequence

Protein

• Length: 439 a.a.
• Molecular weight: 50654.75 Da
• Isoelectric Point: 9.5504

>NTDB_id=166314 AVT04_RS03055 WP_011226886.1 580932..582251(-) (comFA/cflA) [Streptococcus thermophilus strain S9]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKYHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHYLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCREEINAMNKKGGFENEVSTM

Nucleotide

• Length: 1320 bp

>NTDB_id=166314 AVT04_RS03055 WP_011226886.1 580932..582251(-) (comFA/cflA) [Streptococcus thermophilus strain S9]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGTATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATTACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGGAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (290-396)

• Type III restriction enzyme, res subunit (ResIII) (94-206)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA/cflA	Streptococcus pneumoniae Rx1	54.988	98.178	0.54
comFA/cflA	Streptococcus pneumoniae D39	54.988	98.178	0.54
comFA/cflA	Streptococcus pneumoniae R6	54.988	98.178	0.54
comFA/cflA	Streptococcus pneumoniae TIGR4	54.988	98.178	0.54
comFA/cflA	Streptococcus mitis NCTC 12261	54.651	97.95	0.535
comFA/cflA	Streptococcus mitis SK321	53.721	97.95	0.526
comFA	Lactococcus lactis subsp. cremoris KW2	45.714	95.672	0.437
comFA	Latilactobacillus sakei subsp. sakei 23K	37.011	99.089	0.367