Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC18_RS01645 Genome accession   NZ_CP013826
Coordinates   334588..335604 (+) Length   338 a.a.
NCBI ID   WP_029802009.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_018     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329588..340604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC18_RS01630 (FORC18_0267) - 330012..330740 (-) 729 WP_015297397.1 redoxin family protein -
  FORC18_RS01635 (FORC18_0268) oxyR 330911..331819 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC18_RS01640 (FORC18_0269) - 331875..334445 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  FORC18_RS01645 (FORC18_0270) pilM 334588..335604 (+) 1017 WP_029802009.1 type IV pilus biogenesis protein PilM Machinery gene
  FORC18_RS01650 (FORC18_0271) pilN 335588..336166 (+) 579 WP_053046790.1 PilN domain-containing protein Machinery gene
  FORC18_RS01655 (FORC18_0272) pilO 336159..336752 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC18_RS01660 (FORC18_0273) pilP 336742..337257 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  FORC18_RS01665 (FORC18_0274) pilQ 337289..339037 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  FORC18_RS01670 (FORC18_0275) aroK 339226..339744 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37778.28 Da        Isoelectric Point: 6.8323

>NTDB_id=164882 FORC18_RS01645 WP_029802009.1 334588..335604(+) (pilM) [Vibrio parahaemolyticus strain FORC_018]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGSKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=164882 FORC18_RS01645 WP_029802009.1 334588..335604(+) (pilM) [Vibrio parahaemolyticus strain FORC_018]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGTCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATTAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.665

99.704

0.485

Multiple sequence alignment