Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BE842_RS02275 Genome accession   NZ_CP016870
Coordinates   488270..489754 (-) Length   494 a.a.
NCBI ID   WP_011215352.1    Uniprot ID   I7HJU3
Organism   Legionella pneumophila subsp. pneumophila strain FFI103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483270..494754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BE842_RS02260 (BE842_02260) cysS 484272..485641 (+) 1370 Protein_448 cysteine--tRNA ligase -
  BE842_RS02265 (BE842_02265) - 485813..487726 (+) 1914 WP_011215350.1 DUF3413 domain-containing protein -
  BE842_RS02270 (BE842_02270) - 487855..488169 (+) 315 WP_011215351.1 DUF6482 family protein -
  BE842_RS02275 (BE842_02275) pilB 488270..489754 (-) 1485 WP_011215352.1 GspE family T2SS ATPase variant LspE Machinery gene
  BE842_RS02280 (BE842_02280) lspD 489758..492133 (-) 2376 WP_070322060.1 GspD family T2SS secretin variant LspD -
  BE842_RS02285 (BE842_02285) - 492306..493334 (+) 1029 WP_011215354.1 Gfo/Idh/MocA family protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55023.40 Da        Isoelectric Point: 6.0364

>NTDB_id=164668 BE842_RS02275 WP_011215352.1 488270..489754(-) (pilB) [Legionella pneumophila subsp. pneumophila strain FFI103]
MENEEKSLKIPYSFAKGNGLVVGEIKENLAIVYHLPNTPLQAFAEIKRLLQCELDLKQVDETTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYENRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPKHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRQEE
KELIGLKPDADVSQVFEPKGCDSCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLSG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=164668 BE842_RS02275 WP_011215352.1 488270..489754(-) (pilB) [Legionella pneumophila subsp. pneumophila strain FFI103]
ATGGAAAATGAAGAAAAGTCACTTAAAATTCCTTACAGTTTTGCCAAAGGCAATGGACTTGTTGTGGGAGAAATAAAAGA
AAATCTGGCTATTGTCTATCACCTACCTAACACACCTCTGCAAGCGTTCGCCGAGATAAAGCGATTATTGCAATGCGAAC
TTGATCTAAAACAAGTTGATGAAACCACTTTTCAGCAACATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCCGCAGAAGGCATGGAAGAAGATATGGATTTATCTATGTTAGCCAGTCAACTTCCTGTCAGCGAAGACTTATTGGAAAA
TCAAGACGATGCTCCAATTATCCGTTTACTGAATGCTCTGTTCACTCAAGCCATTAAGCAAAAAGCATCTGATATACACA
TCGAAACATATGAAAACAGAGTCTTGGTGCGCAACCGAATTGATGGTGTTCTACAAGAGGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGGATTCCTCAAGATGGTAG
AATTTCATTGCGTATAGGAGGACACAATATTGATGTCAGGGTTTCAACTTTACCATCAAACCATGGAGAACGAGTGGTTT
TAAGGATATTGGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTAAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCTCATGGCATTATTTTAGTCACTGGCCCCACAGGTTCGGGCAAGACAACCTCTCTCTATGCTAT
GCTCACTGAATTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGACCCCATTGAATATGACTTGCCTGGTGTAGGAC
AAACTCAAGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCCATATTACGTCAAGATCCGGATGTTGTC
ATGATAGGAGAGATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCCAGCTTAACAGGGCATTTGGTACTATCAAC
CCTTCATACCAATACTGCATTGGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGGCTCATTGCGCAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCTCATGAATTAAGACAGGAAGAA
AAGGAGTTAATAGGTCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAACCCAAGGGATGCGACTCATGCAACCACTT
GGGTTATAAAGGCAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACAATGGAAAATTATTTACGACCAACCACACCGAGCATACGTGAAGATGGTTTTAAAAGAGTATTATCTGGC
GATACGTCACTTGCAGAAATTTTAAGAGTAACATCCCAAAATTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I7HJU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.75

93.927

0.411
  pilB Vibrio campbellii strain DS40M4

46.798

82.186

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

45.792

81.781

0.374
  pilB Acinetobacter baumannii D1279779

44.471

84.211

0.374
  pilB Acinetobacter baylyi ADP1

47.301

78.745

0.372

Multiple sequence alignment