Detailed information NTG0161955

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ASZ83_RS02585
• Genome accession: NZ_CP013315
• Coordinates: 510128..511003 (-)
• Length: 291 a.a.
• NCBI ID: WP_000418747.1
• Uniprot ID: H9L4S3
• Organism: Vibrio cholerae strain M2140
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 505128..516003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ASZ83_RS02565 (ASZ83_00500)	parC	506078..508363 (+)	2286	WP_000102510.1	DNA topoisomerase IV subunit A	-
ASZ83_RS02570 (ASZ83_00501)	yacG	508412..508609 (-)	198	WP_000162868.1	DNA gyrase inhibitor YacG	-
ASZ83_RS02575 (ASZ83_00502)	zapD	508751..509491 (-)	741	WP_000207198.1	cell division protein ZapD	-
ASZ83_RS02580 (ASZ83_00503)	coaE	509523..510131 (-)	609	WP_000011557.1	dephospho-CoA kinase	-
ASZ83_RS02585 (ASZ83_00504)	pilD	510128..511003 (-)	876	WP_000418747.1	prepilin peptidase	Machinery gene
ASZ83_RS02590 (ASZ83_00505)	pilC	511061..512287 (-)	1227	WP_000648511.1	type II secretion system F family protein	Machinery gene
ASZ83_RS02595 (ASZ83_00506)	-	512330..513271 (-)	942	Protein_467	ATPase, T2SS/T4P/T4SS family	-
ASZ83_RS02600 (ASZ83_00507)	nadC	513252..514007 (-)	756	Protein_468	carboxylating nicotinate-nucleotide diphosphorylase	-
ASZ83_RS02605 (ASZ83_00508)	ampD	514303..514848 (+)	546	WP_000567318.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ASZ83_RS02610 (ASZ83_00509)	fldB	515126..515644 (-)	519	WP_000690112.1	flavodoxin FldB	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32433.69 Da
• Isoelectric Point: 7.1618

>NTDB_id=161955 ASZ83_RS02585 WP_000418747.1 510128..511003(-) (pilD) [Vibrio cholerae strain M2140]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide

• Length: 876 bp

>NTDB_id=161955 ASZ83_RS02585 WP_000418747.1 510128..511003(-) (pilD) [Vibrio cholerae strain M2140]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGTCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTTTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTGTTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGTTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCTGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: H9L4S3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	100	100	1
pilD	Vibrio campbellii strain DS40M4	75.347	98.969	0.746
pilD	Acinetobacter nosocomialis M2	53.676	93.471	0.502
pilD	Acinetobacter baumannii D1279779	53.309	93.471	0.498
pilD	Neisseria gonorrhoeae MS11	44.792	98.969	0.443