Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ATN06_RS05955 Genome accession   NZ_CP013274
Coordinates   1128469..1132194 (+) Length   1241 a.a.
NCBI ID   WP_060629917.1    Uniprot ID   A0A243GJ72
Organism   Bacillus thuringiensis strain CTC     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1123469..1137194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATN06_RS05940 (ATN06_05725) - 1123627..1124220 (+) 594 WP_060629914.1 TVP38/TMEM64 family protein -
  ATN06_RS05945 (ATN06_05730) lepB 1124277..1124840 (+) 564 WP_060629915.1 signal peptidase I -
  ATN06_RS05950 (ATN06_05735) addB 1124957..1128472 (+) 3516 WP_060629916.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ATN06_RS05955 (ATN06_05740) addA 1128469..1132194 (+) 3726 WP_060629917.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ATN06_RS05960 (ATN06_05745) - 1132207..1132494 (+) 288 WP_060629918.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  ATN06_RS05965 (ATN06_05750) gerPF 1132549..1132764 (-) 216 WP_001141566.1 spore germination protein GerPF -
  ATN06_RS05970 (ATN06_05755) - 1132807..1133193 (-) 387 WP_060629919.1 spore germination protein GerPE -
  ATN06_RS05975 (ATN06_05760) gerPD 1133209..1133403 (-) 195 WP_001102341.1 spore germination protein GerPD -
  ATN06_RS05980 (ATN06_05765) gerPC 1133410..1134024 (-) 615 WP_060629920.1 spore germination protein GerPC -
  ATN06_RS05985 (ATN06_05770) gerPB 1134093..1134299 (-) 207 WP_001012508.1 spore germination protein GerPB -
  ATN06_RS05990 (ATN06_05775) gerPA 1134314..1134535 (-) 222 WP_001111187.1 spore germination protein GerPA -
  ATN06_RS05995 (ATN06_05780) - 1134632..1134811 (-) 180 WP_000462840.1 aspartyl-phosphate phosphatase Spo0E family protein -
  ATN06_RS06000 (ATN06_05785) - 1135059..1135958 (+) 900 WP_176225746.1 fumarylacetoacetate hydrolase family protein -
  ATN06_RS06005 (ATN06_05790) - 1135992..1136276 (-) 285 WP_060629921.1 hypothetical protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142800.15 Da        Isoelectric Point: 4.8519

>NTDB_id=161270 ATN06_RS05955 WP_060629917.1 1128469..1132194(+) (addA) [Bacillus thuringiensis strain CTC]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQTDLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQIVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKNNSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEVYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYGYEEATAHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVIAFFES
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIKGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=161270 ATN06_RS05955 WP_060629917.1 1128469..1132194(+) (addA) [Bacillus thuringiensis strain CTC]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCAGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAACGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAGCGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTGGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCGTATTTATTAGAAGATGTGAAATTCCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACTGAACTTGCAATGCTTCCTGACGGTCCAGCACCTCGCGTTGAAACCCTGCAAACAGACTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGTGTGTACGAAGCAATGCAAAATGTGTCATGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGACGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAGGATGAAGTG
AAGAAATTGCAAGAAGAGCTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGATTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAATCGATTTATCGTTTCCGATTAGCAG
AACCAGGCCTATTCCTAGGAAAGTATAAACGTTTCACGCAAGAAGGATTAGGCGGCGGAATGAAAATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGCACGAACTTCATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAGAT
TGACTACGATGCTGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTATTATGTA
TTCAGCAAACGGAAGAAGAAGTACTAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGACCGTAAAAATAATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGCTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTCTTCCGCGTGATTGATAAT
CCGATGCAAGATATTCCACTTGCCGCAGTGCTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCACTTGAAGAAGAACAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGGTGGCGTGAATTCGCGCGCCAACAGTCACTTTCTGATTTG
ATTTGGAAAGTGTACGGTGAGACAGGCTATTACGATTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGACAAGCAAACTT
ACGCGTGCTATATGACCGTGCCAGACAATATGAAGCAACATCGTTTAGAGGGTTATTCCGTTTCTTACGCTTTATCGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTCTAGGTGAACAAGAAGACGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTCGCTGGACTTGGTCGTCGCTTTAATACACAGGATTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCATTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTGAAAGATGCAACGAAGGAAATGGAGAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCTGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAGATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGATGAAGTTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTAGACGGTAACACGTTACTTGCACCAGAGCCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACAGCTCATCGTGCAAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCACGCTTTATGGAGAAGAAAGGGCTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTGAATAAGGAATTATTAACTTTTGAACAAGCAGAAGAAATTGCGATTGAAAAAGTCATTGCGTTCTTTGAAAGT
GATTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGCAAGAGCGACGAAACGATTCTTGTCCAAGGTGTTATCGACTGTATGATTGAAGAGGAAGATG
GTATCACGTTAATCGACTTTAAAACGGATACGATTAAAGGAAAGTTCCCAGGTGGATTCGATCAAGCGAAACCGATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGCTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1060-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243GJ72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.328

100

0.536

Multiple sequence alignment