Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ATN06_RS05950 Genome accession   NZ_CP013274
Coordinates   1124957..1128472 (+) Length   1171 a.a.
NCBI ID   WP_060629916.1    Uniprot ID   A0A243GJF4
Organism   Bacillus thuringiensis strain CTC     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1119957..1133472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATN06_RS05915 (ATN06_05700) - 1120614..1121108 (-) 495 WP_060629910.1 DUF6584 family protein -
  ATN06_RS05920 (ATN06_05705) cspA 1121393..1121596 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  ATN06_RS05925 (ATN06_05710) - 1121785..1121997 (-) 213 WP_060629911.1 hypothetical protein -
  ATN06_RS05930 (ATN06_05715) - 1122326..1122511 (-) 186 WP_060629912.1 hypothetical protein -
  ATN06_RS05935 (ATN06_05720) - 1122795..1123376 (+) 582 WP_060629913.1 competence protein ComK -
  ATN06_RS05940 (ATN06_05725) - 1123627..1124220 (+) 594 WP_060629914.1 TVP38/TMEM64 family protein -
  ATN06_RS05945 (ATN06_05730) lepB 1124277..1124840 (+) 564 WP_060629915.1 signal peptidase I -
  ATN06_RS05950 (ATN06_05735) addB 1124957..1128472 (+) 3516 WP_060629916.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ATN06_RS05955 (ATN06_05740) addA 1128469..1132194 (+) 3726 WP_060629917.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ATN06_RS05960 (ATN06_05745) - 1132207..1132494 (+) 288 WP_060629918.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  ATN06_RS05965 (ATN06_05750) gerPF 1132549..1132764 (-) 216 WP_001141566.1 spore germination protein GerPF -
  ATN06_RS05970 (ATN06_05755) - 1132807..1133193 (-) 387 WP_060629919.1 spore germination protein GerPE -
  ATN06_RS05975 (ATN06_05760) gerPD 1133209..1133403 (-) 195 WP_001102341.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134314.45 Da        Isoelectric Point: 5.1760

>NTDB_id=161269 ATN06_RS05950 WP_060629916.1 1124957..1128472(+) (addB) [Bacillus thuringiensis strain CTC]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNQAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEMHYEARPNEKFHGEASVTIRTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSAKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=161269 ATN06_RS05950 WP_060629916.1 1124957..1128472(+) (addB) [Bacillus thuringiensis strain CTC]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
ACAGCGTCCAAGAGGAGAAACAATATTATATCTTGTGCCAGAACAGATGACATTTCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGGTTAGCGTGGAAAGTATTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGCTACTGGCG
AATAAAGTATATGATTTACAACTACTATATGATGATTTTGAGCGTGCCTTAATCGGAAAATATTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAGTATGTAAATCAGGCTGAAATTTATATAGATGGATTTCATTCGT
TTTCACCGCAAGAGCTAGAAATTGTAAGGCAGCTTATGATTTGCGGAGCGAGAGTTACGATTACGTTAACGATAGATGAA
AAGACGTTAGCACAGCCAGTAAATGAACTAGATCTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
ACGTGAAGAGAAGATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAATGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGTGAAGCGAGTGTAACGATTCGTACAGCAGCTAATTTA
AGAGCTGAAGTAGAAGGTGTAGCCCGTGAAATTCGTAGACTTGTGGCGGACGAAGATTATCGTTACCGTGATATTGCAGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGACCGATGTCACATCATCCGCTAGTAGAATGCATTCGGTCAGCACTCGAGATCATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCAATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTACCGACGCT
ATCGTTCTCTTGACGATACGAATGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGTGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGATGTTCCGAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGCGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAAAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCCACAGGTCTTGAGGCGCTTCAATTCGCCAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCGAATATTGATCGTTCTAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCAGATGAGGAAAGAGATGTTCTTAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGTCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCGTACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACATGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTTACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTGCTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTGTACGGAGATACAATTAAGGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTCGCGCAGCACGGTTTATCATTAAGAGAGCGTGATATTTTCAAATTGGATGC
GCCAGATATCGGGGAGTTATTCCATGCAGCGCTGAAGAGAATTGCGGACAGGCTATTACGTGAAAATCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGCTGTAGTAATAGAAGAAATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATATTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGTGGTACAGGGTCGCTTCCGCCGATGG
AGTTCTCATTACCAAATGGTGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCGGAAGATGAAAACGGA
ACATTCCTACGTATTATTGACTATAAATCAAGTGCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTCGTTACTTCAAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TATTGTACTTCCACATTCATAACCCGATCGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGACTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCTAGTATTGCCAGTGAACAAGAGTTTA
ACGTACTACAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATCGATATTGCTCCT
TACAAAAAGGGAAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
TCAATTCCGTACGCTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(304-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243GJF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

48.976

100

0.49

Multiple sequence alignment