Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AS852_RS08490 Genome accession   NZ_CP013218
Coordinates   1683672..1684358 (-) Length   228 a.a.
NCBI ID   WP_001825349.1    Uniprot ID   -
Organism   Staphylococcus aureus strain LA-MRSA ST398 isolate E154     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678672..1689358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS852_RS08470 (AS852_08170) rpsT 1679586..1679837 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  AS852_RS08475 (AS852_08175) holA 1679882..1680856 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  AS852_RS08480 (AS852_08180) comEC 1680913..1683114 (-) 2202 WP_001807826.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AS852_RS08485 (AS852_08185) - 1683119..1683580 (-) 462 WP_000439690.1 ComE operon protein 2 -
  AS852_RS08490 (AS852_08190) comEA 1683672..1684358 (-) 687 WP_001825349.1 ComEA family DNA-binding protein Machinery gene
  AS852_RS08495 (AS852_08195) - 1684398..1685114 (-) 717 WP_000084838.1 class I SAM-dependent DNA methyltransferase -
  AS852_RS08500 (AS852_08200) rsfS 1685117..1685470 (-) 354 WP_001088020.1 ribosome silencing factor -
  AS852_RS08505 (AS852_08205) yqeK 1685471..1686055 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AS852_RS08510 (AS852_08210) nadD 1686045..1686614 (-) 570 WP_000725164.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  AS852_RS08515 (AS852_08215) yhbY 1686617..1686907 (-) 291 WP_000955234.1 ribosome assembly RNA-binding protein YhbY -
  AS852_RS08520 (AS852_08220) aroE 1686911..1687717 (-) 807 WP_000666746.1 shikimate dehydrogenase -
  AS852_RS08525 (AS852_08225) yqeH 1687731..1688831 (-) 1101 WP_001280151.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25783.41 Da        Isoelectric Point: 9.3305

>NTDB_id=160795 AS852_RS08490 WP_001825349.1 1683672..1684358(-) (comEA) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTALKQSTSENSSLSKLEDVQVKDGDDSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=160795 AS852_RS08490 WP_001825349.1 1683672..1684358(-) (comEA) [Staphylococcus aureus strain LA-MRSA ST398 isolate E154]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATGATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTAGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGCAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

96.476

99.561

0.961
  comEA Staphylococcus aureus N315

96.476

99.561

0.961

Multiple sequence alignment