Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BC375_RS07300 Genome accession   NZ_CP016610
Coordinates   1457865..1459565 (-) Length   566 a.a.
NCBI ID   WP_046419592.1    Uniprot ID   -
Organism   Xylella fastidiosa strain Salento-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1452865..1464565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC375_RS07270 (BC375_06790) - 1453586..1454431 (-) 846 WP_010894024.1 general secretion pathway protein GspK -
  BC375_RS07275 (BC375_06795) - 1454431..1455057 (-) 627 WP_010894023.1 type II secretion system protein J -
  BC375_RS07280 (BC375_06800) - 1455054..1455452 (-) 399 WP_010894022.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BC375_RS07285 (BC375_06805) - 1455449..1455955 (-) 507 WP_010894021.1 Tfp pilus assembly protein FimT/FimU -
  BC375_RS07290 (BC375_06810) gspG 1455961..1456395 (-) 435 WP_004083686.1 type II secretion system major pseudopilin GspG -
  BC375_RS07295 (BC375_06815) - 1456612..1457829 (-) 1218 WP_010894019.1 type II secretion system F family protein -
  BC375_RS07300 (BC375_06820) pilF 1457865..1459565 (-) 1701 WP_046419592.1 type II secretion system ATPase GspE Machinery gene
  BC375_RS07305 (BC375_06825) - 1459857..1462850 (-) 2994 WP_046419589.1 YadA-like family protein -
  BC375_RS07310 (BC375_06830) - 1463553..1464464 (-) 912 WP_046419584.1 AEC family transporter -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62589.37 Da        Isoelectric Point: 5.0845

>NTDB_id=160738 BC375_RS07300 WP_046419592.1 1457865..1459565(-) (pilF) [Xylella fastidiosa strain Salento-2]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPELSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAALQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGVLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFIDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPTHAERYLASPEQVARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDALRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=160738 BC375_RS07300 WP_046419592.1 1457865..1459565(-) (pilF) [Xylella fastidiosa strain Salento-2]
ATGAATGTAAGTATGGAAGCATCCATCGTCGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGCGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGCTGGGTCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTGTCGGCC
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACGGGTGCAGCATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAGGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGATATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATCCAGCGTGCAGTTGAATTACGTGCCTCTGATATCCACATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCGTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATCATGGCCAAGCTTAATATTGCTGAACGTCGCTTGCCTCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGATCGTGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTATTGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTG
CTGGTCACTGGCCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATAGAAGGTATCAATCAGATCCAGGCTAAGCCGCAGATCGGGCTGG
ATTTTGCCAGTGCATTACGCAGCATTGTGCGTCAGGATCCAGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACAGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTTGATATGGGTGTGGAAGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCGCAGCGTTTGGTGC
GTCGCTTGGAGCCTACGCATGCTGAGCGTTATCTTGCCTCGCCAGAGCAGGTTGCGCGTTTTGATTTGCGCCGTTTACAG
CCGGAGGGTGATATTTATTTGTATCGTCCGCGCGCTTCAGCGTTGGCACCCACTGGGTATCTGGGTCGTACGGCGATCGT
CGAATTTCTAATTATGGATGATGCGCTGCGCCGCGCGGTGATCAGCCGTACCGGCATAGGAGAGCTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACCACGATTGAAGAGGTCTTG
CGTGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.794

97.88

0.399
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio parahaemolyticus RIMD 2210633

40.257

96.113

0.387
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Legionella pneumophila strain ERS1305867

36.842

100

0.371