Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   HSISS4_RS01675 Genome accession   NZ_CP013216
Coordinates   328777..330096 (+) Length   439 a.a.
NCBI ID   WP_021143584.1    Uniprot ID   A0AAW7PCJ2
Organism   Streptococcus salivarius strain HSISS4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323777..335096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS01650 (HSISS4_00290) - 324651..325415 (+) 765 WP_021143582.1 ABC transporter ATP-binding protein -
  HSISS4_RS01655 (HSISS4_00291) - 325415..326125 (+) 711 WP_002883761.1 ABC transporter ATP-binding protein -
  HSISS4_RS01660 (HSISS4_00292) - 326260..326919 (+) 660 WP_002890017.1 CBS and ACT domain-containing protein -
  HSISS4_RS01665 (HSISS4_00293) cysK 327068..327994 (-) 927 WP_002883742.1 cysteine synthase A -
  HSISS4_RS01670 (HSISS4_00294) - 328096..328722 (-) 627 WP_021143583.1 YigZ family protein -
  HSISS4_RS01675 (HSISS4_00295) comFA/cflA 328777..330096 (+) 1320 WP_021143584.1 DEAD/DEAH box helicase Machinery gene
  HSISS4_RS01680 (HSISS4_00296) - 330077..330739 (+) 663 WP_021143585.1 ComF family protein -
  HSISS4_RS01685 (HSISS4_00297) hpf 330818..331366 (+) 549 WP_002883786.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50684.98 Da        Isoelectric Point: 9.8112

>NTDB_id=160692 HSISS4_RS01675 WP_021143584.1 328777..330096(+) (comFA/cflA) [Streptococcus salivarius strain HSISS4]
MVPKEYYGRLFTKEQLPVNYHPEALTLEGMIKVDKQLRCKRCYSWIEEDWQLPGGQYYCRACIVFGRNQEGKELYYFPSK
TSEIEFPVLKWFGELTPYQAKVSEKLLRTYQKQKDSLVHAVTGAGKTEMIYNIVAYVLENKGHVAIASPRVDVCRELFLR
MKRDFTCSISLLHAESEPYDGSPLVIATTHQLLKFYQSFDLVIVDEVDAYPFVGNVMLNHAVEQAKTETGRFIYLTATST
LSLEEQVRLGTLEKHHLARRFHGNPLVLPKFFWQGRLQKSLTRGKLPRPLLYQIKKQRKSKFPLLIFFPNIAIGEKFTSI
LQKYLPDEKMAFVSSKSEKRSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPDR
PTGKLYFFHEGLSKSMLRCREEIKVMNKKGGFNNEVSAM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=160692 HSISS4_RS01675 WP_021143584.1 328777..330096(+) (comFA/cflA) [Streptococcus salivarius strain HSISS4]
ATGGTACCTAAAGAATATTATGGACGACTATTTACGAAAGAGCAGTTGCCAGTGAACTATCACCCAGAGGCTTTAACATT
AGAAGGTATGATAAAGGTCGATAAACAACTTAGATGTAAAAGATGTTACAGTTGGATAGAGGAAGATTGGCAATTACCGG
GAGGTCAGTATTATTGTAGAGCATGTATTGTCTTTGGTAGAAACCAAGAAGGAAAAGAACTTTATTATTTTCCTTCCAAA
ACATCAGAAATTGAATTTCCTGTTTTGAAGTGGTTTGGAGAACTGACTCCCTATCAAGCTAAGGTCTCAGAGAAACTTTT
GAGAACTTATCAAAAACAGAAAGATAGTCTCGTACATGCTGTGACCGGTGCTGGTAAGACAGAAATGATTTATAACATCG
TTGCCTATGTTCTGGAAAATAAAGGTCATGTAGCTATCGCAAGTCCTCGGGTTGATGTTTGTCGAGAACTATTTCTACGT
ATGAAAAGGGATTTTACTTGTAGTATTTCCCTGCTTCATGCTGAGAGTGAACCATATGATGGTAGTCCGCTTGTAATAGC
TACCACACATCAACTACTAAAGTTTTACCAGAGTTTTGACTTAGTTATTGTAGATGAAGTAGACGCATATCCCTTTGTAG
GAAATGTCATGTTAAATCATGCTGTAGAACAGGCGAAGACCGAAACCGGTCGATTTATTTACTTAACAGCGACTTCAACA
TTATCTTTAGAAGAGCAAGTTCGACTTGGGACTTTAGAAAAGCATCACCTTGCTAGACGTTTCCATGGAAATCCTTTAGT
ACTTCCTAAATTCTTCTGGCAGGGCAGATTACAAAAATCTCTGACAAGAGGCAAGCTTCCAAGACCTCTTCTTTATCAGA
TTAAAAAGCAACGTAAATCAAAATTTCCTCTATTAATCTTCTTTCCAAATATAGCGATAGGTGAAAAGTTTACAAGTATT
CTACAAAAATATCTTCCAGATGAAAAAATGGCCTTTGTTTCCTCAAAAAGCGAGAAGCGTTCAACTATCGTAGAGAAGTT
CCGAAAAAAAGAACTGTCTATCTTAGTGACAACAACTATCCTCGAACGTGGTGTAACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGAGGAAGAGTTGGAAGATCACCAGATAGA
CCGACAGGAAAGCTTTATTTCTTTCATGAAGGACTATCCAAATCAATGCTTCGGTGTCGAGAAGAGATAAAGGTTATGAA
TAAAAAAGGAGGGTTTAACAATGAAGTGTCTGCTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-169)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

55.916

98.178

0.549
  comFA/cflA Streptococcus pneumoniae D39

55.916

98.178

0.549
  comFA/cflA Streptococcus pneumoniae R6

55.916

98.178

0.549
  comFA/cflA Streptococcus pneumoniae TIGR4

55.916

98.178

0.549
  comFA/cflA Streptococcus mitis NCTC 12261

55.069

98.861

0.544
  comFA/cflA Streptococcus mitis SK321

54.524

98.178

0.535
  comFA Lactococcus lactis subsp. cremoris KW2

46.429

95.672

0.444
  comFA Latilactobacillus sakei subsp. sakei 23K

37.701

99.089

0.374

Multiple sequence alignment