Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GLA29479_RS18445 Genome accession   NZ_CP013141
Coordinates   4525926..4526984 (-) Length   352 a.a.
NCBI ID   WP_057919051.1    Uniprot ID   A0A0S2E2H2
Organism   Lysobacter antibioticus strain ATCC 29479     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4520926..4531984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GLA29479_RS18425 (GLA29479_4158) pilQ 4521775..4523700 (-) 1926 WP_057920190.1 type IV pilus secretin PilQ -
  GLA29479_RS18430 (GLA29479_4159) - 4523878..4524429 (-) 552 WP_057919048.1 pilus assembly protein PilP -
  GLA29479_RS18435 (GLA29479_4160) pilO 4524431..4525132 (-) 702 WP_057919049.1 type 4a pilus biogenesis protein PilO -
  GLA29479_RS18440 (GLA29479_4161) - 4525129..4525926 (-) 798 WP_057972459.1 PilN domain-containing protein -
  GLA29479_RS18445 (GLA29479_4162) pilM 4525926..4526984 (-) 1059 WP_057919051.1 pilus assembly protein PilM Machinery gene
  GLA29479_RS18450 (GLA29479_4163) - 4527388..4529970 (+) 2583 WP_057972460.1 penicillin-binding protein 1A -
  GLA29479_RS18455 (GLA29479_4164) - 4530274..4530927 (+) 654 WP_057919053.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37784.17 Da        Isoelectric Point: 4.6945

>NTDB_id=160290 GLA29479_RS18445 WP_057919051.1 4525926..4526984(-) (pilM) [Lysobacter antibioticus strain ATCC 29479]
MGLITKSQPALVGVDISSTAVKLLQLSRVGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGTRVKHAAAA
VAGSSVITRVIGMPNDLEGDELESQIELEAANYVPYPIDEVNHDFEVLGPMPGSPDMVQVLLAASRSENVEVRASALELG
GLTPRVMDVEAFAIENAFALLADTLSSPRDGLVALVDSGATMTTLNVLRNGRSLYHREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGGASIPRIAEMVEEQLGVPTTVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=160290 GLA29479_RS18445 WP_057919051.1 4525926..4526984(-) (pilM) [Lysobacter antibioticus strain ATCC 29479]
GTGGGACTCATCACGAAAAGCCAGCCGGCGTTGGTCGGCGTGGACATCAGTTCGACGGCGGTGAAGCTGTTGCAACTCTC
ACGAGTCGGCAATCGCTACCGCGTTGAGCACTATGCTGTGGAACCGCTGCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAGGTCGAGGCGGTGGGCGAGGCGATCAAACGTGCGGTCGCGAGGTCGGGGACCCGCGTCAAGCATGCCGCTGCGGCC
GTCGCCGGTTCCTCGGTGATCACCCGCGTGATCGGCATGCCGAACGACCTGGAAGGGGACGAGTTGGAGTCGCAGATCGA
GCTGGAGGCGGCCAATTACGTGCCGTACCCGATCGATGAGGTGAACCACGACTTTGAGGTGCTCGGCCCGATGCCCGGCA
GCCCGGACATGGTCCAGGTATTGCTCGCGGCGTCACGTTCGGAGAATGTCGAGGTTCGCGCTTCGGCGCTGGAGCTCGGC
GGGCTGACGCCCCGCGTCATGGACGTGGAAGCGTTCGCGATCGAGAACGCCTTCGCGTTGCTCGCCGACACCCTGAGCAG
CCCGCGCGACGGTTTGGTCGCCCTGGTCGACTCCGGTGCGACCATGACCACCCTCAACGTACTGCGCAACGGCCGCAGCC
TGTACCACCGCGAGCAGGTCTTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACGGTCTGAGCTACGAGGAA
GCCGGTCTGGCCAAGCGCCAGGGCGGGCTGCCCGAGAGCTACCAGGCCGAGGTGCTGGAGCCGTTCAAGGAAGCGATGGT
CCAGCAGGTCAGCCGCCTGTTGCAGTTCTTCTACGCCGGCAGCGAATTCAACCGTGTCGACCAGATCGTACTGGCCGGCG
GCGGCGCTTCGATTCCGCGCATCGCCGAGATGGTCGAGGAGCAGCTCGGCGTCCCGACCACGGTGGCCAATCCGCTCGCG
CACATGACCCTCGGACCGCGCGTCCAGGCGCATGCGCTCGCACAGGATGCCCCCGCGCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2E2H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.852

100

0.509
  comM Acinetobacter nosocomialis M2

50.568

100

0.506
  comM Acinetobacter baylyi ADP1

50.286

99.432

0.5
  pilM Legionella pneumophila strain ERS1305867

44.571

99.432

0.443

Multiple sequence alignment