Detailed information NTG0160200

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: hsc_RS01655
• Genome accession: NZ_CP013136
• Coordinates: 375492..376355 (-)
• Length: 287 a.a.
• NCBI ID: WP_069372863.1
• Uniprot ID: -
• Organism: Herbaspirillum seropedicae strain AU14040
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 370492..381355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
hsc_RS01625 (Hsc_0340)	argJ	371126..372361 (+)	1236	WP_013232396.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
hsc_RS01630 (Hsc_0341)	-	372370..373245 (+)	876	WP_013232397.1	ATP-binding protein	-
hsc_RS01635 (Hsc_0342)	-	373245..373700 (+)	456	WP_069372861.1	NUDIX domain-containing protein	-
hsc_RS01640 (Hsc_0343)	yacG	373737..373922 (-)	186	WP_013232399.1	DNA gyrase inhibitor YacG	-
hsc_RS01645 (Hsc_0344)	zapD	373952..374707 (-)	756	WP_013232400.1	cell division protein ZapD	-
hsc_RS01650 (Hsc_0345)	coaE	374860..375480 (-)	621	WP_069372862.1	dephospho-CoA kinase	-
hsc_RS01655 (Hsc_0346)	pilD	375492..376355 (-)	864	WP_069372863.1	prepilin peptidase	Machinery gene
hsc_RS01660 (Hsc_0347)	-	376400..377647 (-)	1248	WP_069372864.1	type II secretion system F family protein	-
hsc_RS01665 (Hsc_0348)	pilF	377634..378767 (-)	1134	WP_013232404.1	GspE/PulE family protein	Machinery gene
hsc_RS01670 (Hsc_0349)	-	378931..380235 (-)	1305	WP_069372865.1	HlyC/CorC family transporter	-
hsc_RS01675 (Hsc_0351)	infA	380664..380927 (+)	264	WP_013232407.1	translation initiation factor IF-1	-
hsc_RS01680 (Hsc_0352)	-	381037..381288 (+)	252	WP_069375414.1	hypothetical protein	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 31179.32 Da
• Isoelectric Point: 7.9356

>NTDB_id=160200 hsc_RS01655 WP_069372863.1 375492..376355(-) (pilD) [Herbaspirillum seropedicae strain AU14040]
MPFLEAYALPGDPILCALAALLGLLVGSFLNVLIHRLPIMMLRESANFVAQERGETPPHHDRYDLWLPSSSCPHCATPLP
WRHKLPVLSWLLLRGRCGHCQAAIGLRYPLVELLTAALFAFAAWRFGLGVTGLAVMACTATLVALAFIDAQTMLLPDDLT
LPLMWLGLLVNLTHRLVPLQDAVLGAAAGYGVLWLIFWLFKLITGKDGLGYGDFKLMAALGAWLGWQALPVILLLASAMG
AVLGLVLMAAGRHRRGQAIPFGPCLASAGLLALLQGLDWLWRGLYDY

Nucleotide

• Length: 864 bp

>NTDB_id=160200 hsc_RS01655 WP_069372863.1 375492..376355(-) (pilD) [Herbaspirillum seropedicae strain AU14040]
ATGCCCTTCCTGGAAGCCTACGCCCTCCCTGGCGACCCCATCCTCTGCGCGCTGGCGGCCCTGCTGGGCTTGCTGGTGGG
CAGTTTCCTCAATGTGCTGATCCACCGCCTGCCCATCATGATGCTGCGCGAAAGCGCCAACTTCGTGGCGCAGGAGCGCG
GGGAAACGCCGCCCCATCACGACCGCTACGACCTCTGGCTGCCCTCATCGTCCTGTCCGCACTGCGCCACCCCTCTGCCC
TGGCGTCACAAGCTGCCGGTACTGAGCTGGCTGCTGCTGCGCGGGCGCTGCGGCCACTGCCAGGCCGCCATCGGCCTGCG
TTATCCATTGGTAGAGCTGTTGACGGCGGCGCTGTTCGCCTTCGCCGCCTGGCGCTTCGGCCTGGGCGTCACGGGTCTGG
CCGTGATGGCCTGCACGGCCACGCTGGTCGCGCTGGCCTTCATCGACGCCCAGACCATGCTGCTGCCCGACGACCTGACC
CTGCCGCTGATGTGGCTGGGCCTGCTGGTGAACCTGACGCACCGCCTGGTGCCCCTGCAGGATGCGGTGCTGGGCGCTGC
GGCCGGGTATGGCGTGCTGTGGCTGATCTTCTGGCTCTTCAAGCTCATCACCGGCAAGGATGGGCTGGGCTATGGCGACT
TCAAGCTCATGGCTGCGCTGGGCGCCTGGCTGGGCTGGCAGGCGCTGCCGGTGATCCTGCTGCTGGCCTCGGCCATGGGC
GCGGTGCTAGGGTTGGTGCTGATGGCGGCTGGCCGGCATCGCCGCGGCCAGGCCATTCCCTTCGGACCTTGCCTGGCCAG
CGCCGGCCTCTTGGCGCTGTTGCAGGGACTGGACTGGCTCTGGCGGGGACTTTACGACTACTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.676	94.774	0.509
pilD	Vibrio campbellii strain DS40M4	48.746	97.213	0.474
pilD	Neisseria gonorrhoeae MS11	48.188	96.167	0.463
pilD	Acinetobacter baumannii D1279779	43.956	95.122	0.418
pilD	Acinetobacter nosocomialis M2	43.223	95.122	0.411