Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   DW68_RS18415 Genome accession   NZ_CP013124
Coordinates   3942758..3944350 (+) Length   530 a.a.
NCBI ID   WP_017361287.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina S5.2     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3937758..3949350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DW68_RS18390 (DW68_018390) - 3938780..3939370 (-) 591 WP_026041940.1 TetR/AcrR family transcriptional regulator -
  DW68_RS18395 (DW68_018395) pgeF 3939503..3940234 (-) 732 WP_017361284.1 peptidoglycan editing factor PgeF -
  DW68_RS18400 (DW68_018400) rluD 3940231..3941205 (-) 975 WP_026041941.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  DW68_RS18405 (DW68_018405) - 3941344..3942336 (+) 993 WP_017361286.1 outer membrane protein assembly factor BamD -
  DW68_RS26130 (DW68_018410) - 3942532..3942768 (+) 237 WP_013714194.1 PP0621 family protein -
  DW68_RS18415 (DW68_018415) pilS 3942758..3944350 (+) 1593 WP_017361287.1 PAS domain-containing sensor histidine kinase Regulator
  DW68_RS18420 (DW68_018420) pilR 3944353..3945693 (+) 1341 WP_017361288.1 sigma-54 dependent transcriptional regulator Regulator
  DW68_RS18425 (DW68_018425) thiO 3945710..3946810 (-) 1101 WP_036994836.1 glycine oxidase ThiO -
  DW68_RS26345 - 3946893..3946943 (+) 51 Protein_3647 hypothetical protein -
  DW68_RS18430 (DW68_018430) - 3947013..3947417 (+) 405 WP_169746326.1 GspH/FimT family protein -
  DW68_RS18435 (DW68_018435) - 3947528..3948043 (+) 516 WP_017361291.1 GspH/FimT family pseudopilin -
  DW68_RS18440 (DW68_018440) pilV 3948060..3948626 (+) 567 WP_017361292.1 type IV pilus modification protein PilV -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58991.11 Da        Isoelectric Point: 8.1909

>NTDB_id=160089 DW68_RS18415 WP_017361287.1 3942758..3944350(+) (pilS) [Ectopseudomonas mendocina S5.2]
MTADLSLQGGTQGRRILRLYHFYRLSIGLALILLISSNLDEQFLDVVHIELFRTGSWAYLILNILIAVLVQKPKHLMQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAIAAIALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLQVSESLAEQRAADVANLEELNEQILQRMRTGILVLDTQHRVLLANQGATQLLGRGELTGKIID
PHCPELVKRLQQWLHNPTLRPDSLQAQVDGPVLQPSFISLQRGEQQHILVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPSD
RQIHLETQGGSLQTRMDPHQLTQVLTNLVQNGLRYSAQKHRLGQVWLRLFRDESSDLPVLEILDDGPGVPPEQEQHIFEP
FYTTENKGTGLGLYISRELCQGNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=160089 DW68_RS18415 WP_017361287.1 3942758..3944350(+) (pilS) [Ectopseudomonas mendocina S5.2]
GTGACCGCTGACCTCAGCTTGCAAGGCGGTACGCAGGGGCGACGCATCCTCAGGCTTTATCACTTCTATCGCCTGAGCAT
CGGCCTGGCCCTGATCCTGCTGATATCCAGCAACCTCGATGAACAGTTTCTGGATGTGGTGCATATCGAGCTGTTTCGGA
CAGGCAGCTGGGCCTATCTGATCCTCAACATCCTCATCGCTGTGCTGGTGCAAAAGCCCAAGCACCTGATGCAGCTGTTC
AGCCTGGCGCTGGTGGACGTGATTTTCCTGTCCGCGCTGTTCTATGCCGCTGGCGGCACGCCCAGCGGCATCGGCAACCT
GCTGGTGGTAGCGGTGGCCATCGCCAATATTCTGCTGCGCGGCCGCATCGGCCTGCTGATCGCCGCCATCGCTGCCATCG
CGCTTATTTACCTGACCTTCTATCTCAGCCTCAGCCGCCCCGCCGCTGCCGCCCAGTACGTGCAGGCCGGCGCACTTGGC
GCGCTGTGTTTCGCCGGCGCACTGTTTATCCAGGGCATCACTCGCCGCCTGCAGGTCAGCGAAAGCCTGGCCGAGCAACG
CGCGGCCGATGTCGCCAACCTGGAAGAGCTCAACGAGCAGATATTGCAGCGCATGCGCACCGGTATCCTGGTGCTCGATA
CCCAGCATCGCGTGCTCCTGGCCAACCAGGGCGCTACGCAACTGCTGGGCCGCGGGGAGCTGACCGGCAAGATCATCGAC
CCGCACTGCCCGGAACTGGTCAAACGGCTGCAACAGTGGCTGCACAACCCGACACTGCGCCCGGACAGCCTTCAGGCGCA
AGTCGATGGCCCGGTCCTGCAGCCCAGTTTCATTTCCCTGCAACGCGGCGAGCAGCAGCACATCCTGGTGTTTCTCGACG
ACATTTCGCAGATTGCCCAGCAGGCGCAACAGCTCAAACTGGCCTCGCTCGGCCGCTTGACCGCCGGTATCGCCCACGAG
ATTCGCAACCCGCTCGGCGCCATCAGCCACGCCGCGCAGTTGTTGCAGGAGTCCGAAGACCTGCAAGGCCCGGATCAGCG
CCTGGCGCAAATCATTCAGGATCACTCCAAGCGCATGAATCTGGTGATCGAGAACGTGCTACAGCTGTCGCGCCGGCGCC
AGGCCGAGCCACAGCTGCTGGATCTCAAATACTGGCTGCACCGCTTCGCCAGCGAGTTTCGCAGCTCGGCCCCAAGCGAC
CGGCAGATCCACCTGGAAACCCAGGGCGGCAGCCTGCAAACACGCATGGACCCGCACCAGTTGACCCAGGTGCTGACCAA
CCTGGTGCAGAACGGCTTACGCTACAGCGCGCAGAAACATCGACTCGGCCAGGTCTGGCTACGCCTGTTCCGCGACGAGA
GCAGCGACCTGCCAGTGCTGGAAATCCTCGACGACGGCCCCGGTGTGCCGCCCGAGCAGGAACAGCACATCTTCGAGCCC
TTCTACACCACCGAGAACAAGGGCACCGGCCTCGGCCTGTATATTTCCCGCGAACTGTGCCAGGGCAACCAGGCCCGCCT
CGACTATAAACCTCGTGAAGGTGGCGGCAGTTGCTTCCGCATCACCTTCGCCCATCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

PAS domain (PAS_8)

(199-254)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.63

97.925

0.662

Multiple sequence alignment