Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACT75_RS03415 Genome accession   NZ_CP012959
Coordinates   736821..737372 (+) Length   183 a.a.
NCBI ID   WP_005551171.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 731821..742372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT75_RS03400 (ACT75_03400) - 732850..734214 (+) 1365 WP_005542466.1 L-serine ammonia-lyase -
  ACT75_RS03405 (ACT75_03405) ansB 734316..735365 (-) 1050 WP_005542467.1 L-asparaginase 2 -
  ACT75_RS03410 (ACT75_03410) - 735639..736799 (+) 1161 WP_005594622.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACT75_RS03415 (ACT75_03415) pilF/pilF2 736821..737372 (+) 552 WP_005551171.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACT75_RS03420 (ACT75_03420) - 737515..738570 (+) 1056 WP_005542473.1 RodZ domain-containing protein -
  ACT75_RS03425 (ACT75_03425) ispG 738582..739685 (+) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACT75_RS03430 (ACT75_03430) hisS 739707..740984 (+) 1278 WP_005551169.1 histidine--tRNA ligase -
  ACT75_RS03435 (ACT75_03435) - 740995..741609 (+) 615 WP_005539337.1 YfgM family protein -
  ACT75_RS03440 (ACT75_03440) - 741662..742114 (+) 453 WP_005539334.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20594.34 Da        Isoelectric Point: 8.0841

>NTDB_id=158598 ACT75_RS03415 WP_005551171.1 736821..737372(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 624]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKACSYAPDYYLVSAAFAYF
YQQQGDIEQARAAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPERAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=158598 ACT75_RS03415 WP_005551171.1 736821..737372(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 624]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAACAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTGTTCTTACGCTCCAGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTGCCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTCCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTACGAAAACTTGGCGCTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCCCCGGAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

56.97

90.164

0.514
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437

Multiple sequence alignment