Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACT74_RS06810 Genome accession   NZ_CP012958
Coordinates   1447770..1448321 (+) Length   183 a.a.
NCBI ID   WP_005547469.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain VT1169     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1442770..1453321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT74_RS06795 (ACT74_06790) - 1443720..1445084 (+) 1365 WP_005547462.1 L-serine ammonia-lyase -
  ACT74_RS06800 (ACT74_06795) ansB 1445265..1446314 (-) 1050 WP_050846138.1 L-asparaginase 2 -
  ACT74_RS06805 (ACT74_06800) - 1446588..1447748 (+) 1161 WP_005586654.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACT74_RS06810 (ACT74_06805) pilF/pilF2 1447770..1448321 (+) 552 WP_005547469.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACT74_RS06815 (ACT74_06810) - 1448465..1449520 (+) 1056 WP_005568019.1 RodZ domain-containing protein -
  ACT74_RS06820 (ACT74_06815) ispG 1449532..1450635 (+) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACT74_RS06825 (ACT74_06820) hisS 1450657..1451934 (+) 1278 WP_005589329.1 histidine--tRNA ligase -
  ACT74_RS06830 (ACT74_06825) - 1451945..1452559 (+) 615 WP_014167419.1 YfgM family protein -
  ACT74_RS06835 (ACT74_06830) - 1452612..1453064 (+) 453 WP_005568016.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20667.46 Da        Isoelectric Point: 8.8869

>NTDB_id=158578 ACT74_RS06810 WP_005547469.1 1447770..1448321(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain VT1169]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=158578 ACT74_RS06810 WP_005547469.1 1447770..1448321(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain VT1169]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437

Multiple sequence alignment