Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   A9Z04_RS10035 Genome accession   NZ_CP016007
Coordinates   2069915..2070592 (+) Length   225 a.a.
NCBI ID   WP_000178166.1    Uniprot ID   -
Organism   Escherichia coli strain NGF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2064915..2075592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9Z04_RS10000 (A9Z04_10000) gspG 2065395..2065832 (+) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -
  A9Z04_RS10005 (A9Z04_10005) gspH 2065840..2066349 (+) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  A9Z04_RS10010 (A9Z04_10010) gspI 2066346..2066723 (+) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  A9Z04_RS10015 (A9Z04_10015) gspJ 2066716..2067303 (+) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  A9Z04_RS10020 (A9Z04_10020) gspK 2067296..2068279 (+) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -
  A9Z04_RS10025 (A9Z04_10025) gspL 2068294..2069457 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  A9Z04_RS10030 (A9Z04_10030) gspM 2069454..2069915 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  A9Z04_RS10035 (A9Z04_10035) pilD 2069915..2070592 (+) 678 WP_000178166.1 prepilin peptidase GspO Machinery gene
  A9Z04_RS10040 (A9Z04_10040) bfr 2070621..2071097 (-) 477 WP_000675504.1 bacterioferritin -
  A9Z04_RS10045 (A9Z04_10045) bfd 2071169..2071363 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  A9Z04_RS10050 (A9Z04_10050) chiA 2071532..2074234 (-) 2703 WP_000773168.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24814.64 Da        Isoelectric Point: 7.6364

>NTDB_id=158496 A9Z04_RS10035 WP_000178166.1 2069915..2070592(+) (pilD) [Escherichia coli strain NGF1]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLAHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=158496 A9Z04_RS10035 WP_000178166.1 2069915..2070592(+) (pilD) [Escherichia coli strain NGF1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGCTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382

Multiple sequence alignment