Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACH32_RS00800 Genome accession   NZ_CP012756
Coordinates   151588..152274 (-) Length   228 a.a.
NCBI ID   WP_072468915.1    Uniprot ID   A0A5F0HFN7
Organism   Staphylococcus aureus strain JS395     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146588..157274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH32_RS00780 (ACH32_00775) rpsT 147502..147753 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  ACH32_RS00785 (ACH32_00780) holA 147798..148772 (-) 975 WP_001282573.1 DNA polymerase III subunit delta -
  ACH32_RS00790 (ACH32_00785) comEC 148829..151030 (-) 2202 WP_025174621.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACH32_RS00795 (ACH32_00790) - 151035..151496 (-) 462 WP_000439693.1 ComE operon protein 2 -
  ACH32_RS00800 (ACH32_00795) comEA 151588..152274 (-) 687 WP_072468915.1 ComEA family DNA-binding protein Machinery gene
  ACH32_RS00805 (ACH32_00800) - 152314..153030 (-) 717 WP_000084840.1 class I SAM-dependent DNA methyltransferase -
  ACH32_RS00810 (ACH32_00805) rsfS 153033..153386 (-) 354 WP_001088020.1 ribosome silencing factor -
  ACH32_RS00815 (ACH32_00810) yqeK 153387..153971 (-) 585 WP_001017838.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACH32_RS00820 (ACH32_00815) nadD 153961..154530 (-) 570 WP_000725165.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  ACH32_RS00825 (ACH32_00820) yhbY 154533..154823 (-) 291 WP_000955234.1 ribosome assembly RNA-binding protein YhbY -
  ACH32_RS00830 (ACH32_00825) aroE 154827..155633 (-) 807 WP_000666754.1 shikimate dehydrogenase -
  ACH32_RS00835 (ACH32_00830) yqeH 155647..156747 (-) 1101 WP_001280146.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25812.41 Da        Isoelectric Point: 9.3305

>NTDB_id=157602 ACH32_RS00800 WP_072468915.1 151588..152274(-) (comEA) [Staphylococcus aureus strain JS395]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTALKQSTSENSSLSKLEDVQAKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGENSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=157602 ACH32_RS00800 WP_072468915.1 151588..152274(-) (comEA) [Staphylococcus aureus strain JS395]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGCCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGAAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HFN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

96.476

99.561

0.961
  comEA Staphylococcus aureus N315

96.476

99.561

0.961

Multiple sequence alignment