Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AK973_RS26700 Genome accession   NZ_CP012680
Coordinates   6108467..6110056 (+) Length   529 a.a.
NCBI ID   WP_060614143.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain LBUM300     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 6103467..6115056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK973_RS26680 (AK973_5325) pgeF 6105267..6105992 (-) 726 WP_003205694.1 peptidoglycan editing factor PgeF -
  AK973_RS26685 (AK973_5326) rluD 6105989..6106951 (-) 963 WP_003205696.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AK973_RS26690 (AK973_5327) - 6107102..6108118 (+) 1017 WP_003205698.1 outer membrane protein assembly factor BamD -
  AK973_RS26695 - 6108244..6108477 (+) 234 WP_013694237.1 PP0621 family protein -
  AK973_RS26700 (AK973_5329) pilS 6108467..6110056 (+) 1590 WP_060614143.1 two-component system sensor histidine kinase NtrB Regulator
  AK973_RS26705 (AK973_5330) pilR 6110053..6111399 (+) 1347 WP_053163367.1 sigma-54-dependent transcriptional regulator Regulator
  AK973_RS26710 - 6111481..6111846 (-) 366 WP_038858257.1 DUF6124 family protein -
  AK973_RS26715 (AK973_5332) - 6112527..6113957 (-) 1431 WP_039011935.1 IS1182 family transposase -
  AK973_RS26720 (AK973_5333) - 6113956..6114141 (+) 186 WP_060614144.1 hypothetical protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58922.77 Da        Isoelectric Point: 7.3882

>NTDB_id=156332 AK973_RS26700 WP_060614143.1 6108467..6110056(+) (pilS) [Pseudomonas brassicacearum strain LBUM300]
MSAEASSPREKQAQRLLRLYHLYRLSIGITLVLLISSKLDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGVPSAIGNLLIVSVAIGNTLLRSRVGLLIAAVATIGIVGSTFFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLVGQRID
DYCTALVERLQLWLNNPSLRPPSLTVTGTGLTLQPSFIALGHHDQQQLLVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNNADRRLTQIIQDHSQRMNRVIENVLLLSRRQQTTPQRLDLRTWLDQFVRQARESMAEH
QHLHLSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAMTHEQAEAWLKLFIDPHSQLSTLDIIDNGPGVTPEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=156332 AK973_RS26700 WP_060614143.1 6108467..6110056(+) (pilS) [Pseudomonas brassicacearum strain LBUM300]
GTGAGCGCTGAGGCCTCAAGCCCACGCGAAAAACAGGCGCAGCGCCTGCTGCGTCTCTATCACCTGTATCGCCTGAGCAT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAAACTGGACAACCGCCTGCTGGAGTTCGCCAATGACGACCTGCTGCGCA
GTGGCAGTTGGTTGTACCTGGTGCTGAATATCCTGCTGGTGGTGTTCCTTGAGAACACCCGCCGCCCTGCCCGGCTGTTC
GGCCTGGCCCTGACTGACGTGCTGCTGCTCTCGTGGCTGTTCTTCGCCGCGGGCGGTGTGCCCAGTGCCATCGGCAACCT
GCTCATTGTCTCGGTGGCCATCGGCAATACGCTGTTGCGAAGCCGGGTCGGCCTGTTGATTGCCGCTGTCGCCACGATCG
GTATCGTCGGGTCGACTTTTTTCCTCGGCCTCAGCGACTCAAACCGTCCCAGCAGTTATCTGCAAGCCGGCACCCTGGGG
GCACTGTGTTTTGCCGCCGCCCTGCTGGTACAGGGCCTGACCCGGCGACTGGAAGCCAGTGAAACCCTGGCCGAGCAACG
GGCCAGCGAGGTAATCGGCCTCGAAGCGCTCAACGCACTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTCGACC
GCGAACGGCGCGTGCAATTGGCCAACGAAAGCGCCTTGAACCTGCTGGGCATGCACGACCTGGTCGGCCAGCGAATCGAT
GACTATTGCACCGCCCTGGTCGAACGCCTGCAATTGTGGCTGAACAACCCCAGCCTGCGCCCCCCCAGCCTGACCGTGAC
CGGCACAGGCCTGACCCTGCAACCGAGCTTCATCGCCTTGGGGCATCACGATCAGCAGCAGTTGCTGGTATTCCTTGAGG
ACCTGGCCCAAGTTGCCCAGCAGGCCCAGCAACTGAAACTCGCGTCCCTCGGGCGCCTCACTGCTGGCATCGCCCATGAA
ATCCGCAACCCCTTGGGCGCCATCAGCCACGCAGCGCAATTACTGCGCGAATCCGAGGAACTGAACAACGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAACGAATGAATCGCGTCATTGAAAACGTCCTGCTGCTGTCTCGCCGCCAGC
AAACCACCCCCCAACGCCTCGACCTGCGGACCTGGCTCGATCAGTTCGTCCGGCAGGCCCGCGAAAGCATGGCCGAGCAC
CAGCACTTGCACCTGAGCATCGACCCGGGTGACTACACCACGCTGATGGACCCCGACCAGTTGACCCAGGTACTCGACAA
CCTGCTGCGCAACGCCTGGCGCCACAGCGCCATGACCCACGAACAGGCCGAGGCCTGGCTGAAACTGTTCATCGATCCCC
ACAGCCAGCTGTCCACACTGGACATCATCGACAACGGCCCCGGTGTAACACCCGAGCAGCAGGCACATTTGTTCGAACCC
TTCTTTACCACCAGCAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAGAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGTTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.848

99.811

0.597

Multiple sequence alignment