Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   RN80_RS00520 Genome accession   NZ_CP012646
Coordinates   96575..97399 (-) Length   274 a.a.
NCBI ID   WP_049543728.1    Uniprot ID   -
Organism   Streptococcus mitis strain KCOM 1350 (= ChDC B183)     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 91575..102399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN80_RS00505 (RN80_00510) amiA 92570..94549 (-) 1980 WP_020902007.1 peptide ABC transporter substrate-binding protein Regulator
  RN80_RS00515 (RN80_00520) - 94975..96306 (-) 1332 WP_049543730.1 hemolysin family protein -
  RN80_RS00520 (RN80_00525) endA 96575..97399 (-) 825 WP_049543728.1 DNA-entry nuclease EndA Machinery gene
  RN80_RS00525 (RN80_00530) - 97438..97626 (-) 189 WP_025169706.1 DNA-directed RNA polymerase subunit beta -
  RN80_RS00530 (RN80_00535) murA 97619..98902 (-) 1284 WP_060627156.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  RN80_RS00535 (RN80_00540) - 98926..99156 (-) 231 WP_000250396.1 DUF1146 family protein -
  RN80_RS00540 (RN80_00545) sepM 99214..100251 (-) 1038 WP_060627157.1 SepM family pheromone-processing serine protease Regulator
  RN80_RS00545 (RN80_00550) coaD 100235..100723 (-) 489 WP_060627158.1 pantetheine-phosphate adenylyltransferase -
  RN80_RS00550 (RN80_00555) rsmD 100713..101252 (-) 540 WP_060627159.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  RN80_RS00555 (RN80_00560) asnA 101317..102309 (-) 993 WP_060627160.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29893.55 Da        Isoelectric Point: 9.9431

>NTDB_id=155969 RN80_RS00520 WP_049543728.1 96575..97399(-) (endA) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MDKKTRQTLIGLLVLLLLSAGSYYIKQMQSTANSPKTKLSQKKQASEAPSQALAESVLTDAVKSQVKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSNSWTPPGWHQVKNLKGTYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKALDKNKRVRYRVTLYYATNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=155969 RN80_RS00520 WP_049543728.1 96575..97399(-) (endA) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGGATAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTGCAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCCAAAACCAAGCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAGTAAAAGGGAGTCTGGAGTGGAATGGCTCGGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAACCCTACGCTGATAATAAAACAAAGACAGTGGGCAAGGA
AACCGTGCCAACTGTAGCAAATGCCCTCTTGTCTAAGGCTACTCGCCAGTATAAAAATCGTGAAGAAACGGGGAATGGTT
CGAATTCATGGACTCCTCCGGGATGGCATCAGGTTAAGAATCTAAAGGGGACTTATACTCATGCGGTCGATAGAGGTCAT
TTATTAGGCTATGCCTTGATAGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAATATTGCTGTTCAAAC
AGCCTGGGCAAATCAGGCGCAAGCCGAGGATTCGACAGGGCAAAACTACTATGAAAGCATGGTACGCAAGGCCTTGGATA
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTATTACGCTACAAATGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGACGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGGCTTCAACTGGATTACCGAAC
TGGAGAAGTAACGGTCACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

94.891

100

0.949
  endA Streptococcus pneumoniae D39

94.891

100

0.949
  endA Streptococcus pneumoniae R6

94.891

100

0.949
  endA Streptococcus pneumoniae TIGR4

94.891

100

0.949

Multiple sequence alignment