Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACN91_RS27290 Genome accession   NZ_CP012483
Coordinates   5329580..5330956 (-) Length   458 a.a.
NCBI ID   WP_053565452.1    Uniprot ID   A0A9X7GKN7
Organism   Bacillus cereus strain NJ-W     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5324580..5335956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN91_RS27270 (ACN91_27305) ispF 5325949..5326425 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACN91_RS27275 (ACN91_27310) ispD 5326542..5327222 (-) 681 WP_053565450.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACN91_RS27280 (ACN91_27315) - 5327239..5328342 (-) 1104 WP_053565451.1 PIN/TRAM domain-containing protein -
  ACN91_RS27285 (ACN91_27320) disA 5328503..5329576 (-) 1074 WP_000392164.1 DNA integrity scanning diadenylate cyclase DisA -
  ACN91_RS27290 (ACN91_27325) radA 5329580..5330956 (-) 1377 WP_053565452.1 DNA repair protein RadA Machinery gene
  ACN91_RS27295 (ACN91_27330) clpC 5331052..5333487 (-) 2436 WP_000971170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  ACN91_RS27300 (ACN91_27335) - 5333510..5334574 (-) 1065 WP_000050836.1 protein arginine kinase -
  ACN91_RS27305 (ACN91_27340) - 5334579..5335127 (-) 549 WP_000128385.1 UvrB/UvrC motif-containing protein -
  ACN91_RS27310 (ACN91_27345) ctsR 5335298..5335759 (-) 462 WP_001244560.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49971.66 Da        Isoelectric Point: 6.7002

>NTDB_id=154729 ACN91_RS27290 WP_053565452.1 5329580..5330956(-) (radA) [Bacillus cereus strain NJ-W]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETEFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPDEIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=154729 ACN91_RS27290 WP_053565452.1 5329580..5330956(-) (radA) [Bacillus cereus strain NJ-W]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGTAAATGTCCTGGATG
TGGTCAATGGAATACACTTGTTGAAGAAATGGAGCCAGTTGTATCATCTAGACGCCTTAATTACGCGAATGCAATTCAAA
CGGAAGTAACAAAACCAAGACGTCTTACTGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACGGAATTTCAAGAGTTT
AATCGTGTGCTTGGTGGTGGAATTGTAGATGGGTCCTTAGTGCTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
ACTATTACAGATTTCATCACAATTAGCAGATTCTTCATATGATGTATTATACATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGCAATCTATTTGTTGTAGCAGAGACAGACCTGCAGCGTATTGCA
GCACATATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
ACCAGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCTATTTTCA
TTGTCGGGCATGTGACAAAAGAAGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACGTATCGTATTTTACGGGCTGTGAAGAACCGTTTTGGTTCCACGAATGAAATGGGTATCTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTTTTAAATCCTTCTGAAATCTTCCTTGAGGAAAGGCCGGTTGGTGTTGCAG
GATCAACAGTAGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTTTCGCTTATTATGGCAGTATTAGAAAAAAGAACAGG
TTTACTATTGCAAAATCAAGATGCATATTTAAAAGTAGCTGGTGGTTTGAAATTAGACGAACCGGCAATTGATTTAGCAG
TGGCTTTAAGTATAGCCTCAAGTTTTAGGGATAAATCTACGGCACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGTTATTATTCC
TAGAAAAAACTTAGGAGGATGGACAATTCCGGATGAGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.459

98.908

0.638
  radA Streptococcus pneumoniae D39

64.459

98.908

0.638
  radA Streptococcus pneumoniae R6

64.459

98.908

0.638
  radA Streptococcus pneumoniae TIGR4

64.459

98.908

0.638
  radA Streptococcus mitis SK321

64.459

98.908

0.638
  radA Streptococcus mitis NCTC 12261

64.238

98.908

0.635

Multiple sequence alignment