Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACN91_RS06665 Genome accession   NZ_CP012483
Coordinates   1277367..1278410 (+) Length   347 a.a.
NCBI ID   WP_053563650.1    Uniprot ID   A0A9W3XAM4
Organism   Bacillus cereus strain NJ-W     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1272367..1283410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN91_RS06630 (ACN91_06630) - 1272732..1273220 (+) 489 WP_000764263.1 hypothetical protein -
  ACN91_RS06635 (ACN91_06635) - 1273255..1273566 (-) 312 WP_001093240.1 hypothetical protein -
  ACN91_RS06640 (ACN91_06640) - 1273820..1274035 (+) 216 WP_001008326.1 DUF3912 family protein -
  ACN91_RS06645 (ACN91_06645) - 1274123..1274515 (+) 393 WP_053563649.1 hypothetical protein -
  ACN91_RS06650 (ACN91_06650) - 1274595..1275989 (-) 1395 WP_001094344.1 L-cystine transporter -
  ACN91_RS06655 (ACN91_06655) - 1276097..1276339 (-) 243 WP_000375619.1 DUF2626 domain-containing protein -
  ACN91_RS06660 (ACN91_06660) - 1276466..1277161 (-) 696 WP_000434107.1 helix-turn-helix domain-containing protein -
  ACN91_RS06665 (ACN91_06665) comGA 1277367..1278410 (+) 1044 WP_053563650.1 competence type IV pilus ATPase ComGA Machinery gene
  ACN91_RS06670 (ACN91_06670) comGB 1278397..1279434 (+) 1038 WP_053563651.1 competence type IV pilus assembly protein ComGB -
  ACN91_RS06675 (ACN91_06675) comGC 1279446..1279745 (+) 300 WP_053563652.1 competence type IV pilus major pilin ComGC -
  ACN91_RS06680 (ACN91_06680) comGD 1279742..1280197 (+) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  ACN91_RS06685 (ACN91_06685) - 1280190..1280492 (+) 303 WP_000829462.1 hypothetical protein -
  ACN91_RS06690 (ACN91_06690) comGF 1280462..1280932 (+) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  ACN91_RS06695 (ACN91_06695) comGG 1280929..1281300 (+) 372 WP_023523011.1 competence type IV pilus minor pilin ComGG -
  ACN91_RS06700 (ACN91_06700) - 1281475..1282125 (+) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  ACN91_RS06705 (ACN91_06705) - 1282241..1282738 (+) 498 WP_053563653.1 shikimate kinase -
  ACN91_RS06710 (ACN91_06710) - 1282777..1282977 (+) 201 WP_000106083.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39178.54 Da        Isoelectric Point: 8.5432

>NTDB_id=154702 ACN91_RS06665 WP_053563650.1 1277367..1278410(+) (comGA) [Bacillus cereus strain NJ-W]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRVGKDLMTRHCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=154702 ACN91_RS06665 WP_053563650.1 1277367..1278410(+) (comGA) [Bacillus cereus strain NJ-W]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTGTAGGAAAAGATTTAATGACGAGACATTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCTAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCATCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.441

92.795

0.366

Multiple sequence alignment