Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DE8669_RS00255 Genome accession   NZ_CP012391
Coordinates   44234..45499 (+) Length   421 a.a.
NCBI ID   WP_002228837.1    Uniprot ID   A0AB33TWV1
Organism   Neisseria meningitidis strain DE8669     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 39234..50499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DE8669_RS00235 (DE8669_0047) - 39964..40965 (+) 1002 WP_002238540.1 thiamine ABC transporter substrate-binding protein -
  DE8669_RS00240 (DE8669_0048) - 41059..41907 (+) 849 WP_002215243.1 mechanosensitive ion channel family protein -
  DE8669_RS00245 (DE8669_0049) - 41933..42418 (+) 486 WP_002221794.1 CinA family protein -
  DE8669_RS00250 (DE8669_0050) msrAB 42520..44088 (-) 1569 WP_002216163.1 bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB -
  DE8669_RS00255 (DE8669_0051) pilA 44234..45499 (+) 1266 WP_002228837.1 signal recognition particle-docking protein FtsY Machinery gene
  DE8669_RS00260 (DE8669_0052) pilC 45744..48929 (+) 3186 WP_079870325.1 PilC family type IV pilus tip adhesin Machinery gene

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 45201.50 Da        Isoelectric Point: 4.5585

>NTDB_id=154053 DE8669_RS00255 WP_002228837.1 44234..45499(+) (pilA) [Neisseria meningitidis strain DE8669]
MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVESLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAE
EAAEKAAEQVEAAKEAVAETVGEAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPFVIMLAGINGAGKTTSIGK
LAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNNVTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHL
MEEIKKVKRVLQKAMPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVRYIGV
GEGIDDLRPFDARAFVDALLD

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=154053 DE8669_RS00255 WP_002228837.1 44234..45499(+) (pilA) [Neisseria meningitidis strain DE8669]
ATGTTCAGCTTCTTCCGTCGCAAGAAAAAACAGGAAACGCCGGCTCCCGAGGAGGCTCAAGTTCAGGAAACTGCAGCAAA
AGCAGAATCCGAAGTTACTCAAATAGTTGAAAATATTAAAGAAGAAGTCGAATCTTTAACGGAAAGCGTCAAAGGGCAGG
TCGAATCGGCTGTTGAAACCGTCAGCGGTGCGGTTGAACAGGTAAAGGAAACCGTTGCCGAGATGCTGTCTGAAGCAGAG
GAAGCGGCGGAAAAAGCAGCGGAACAAGTCGAAGCGGCAAAAGAAGCCGTTGCCGAAACCGTCGGCGAGGCTGTCGGGCA
AGTTCAAGAAGCCGTTGCGACAACTGAAGAACACAAGCTCGGTTGGGCGGCGCGTTTGAAACAAGGTTTGACCAAATCGC
GCGACAAAATGGCGAAATCGCTGGCGGGCGTGTTCGGCGGCGGACAAATCGACGAAGATTTGTACGAAGAGCTGGAAACC
GTGCTGATTACCAGCGATATGGGCATGGAAGCCACCGAATACCTGATGAAAGACGTGCGCGACCGCGTCAGCCTCAAAGG
GCTGAAAGACGGCAACGAATTGCGCGGCGCGCTGAAAGAAGCCTTGTACGACCTGATTAAGCCTCTAGAGAAACCGCTGG
TTTTGCCCGAAACCAAAGAGCCGTTTGTCATCATGCTTGCTGGCATCAACGGCGCGGGCAAAACCACATCCATCGGCAAA
CTCGCCAAATATTTCCAAGCGCAGGGCAAATCTGTGCTGCTTGCCGCCGGCGATACTTTCCGCGCCGCCGCGCGTGAGCA
GCTTCAAGCTTGGGGCGAACGCAACAACGTAACCGTGATTTCGCAAACCACGGGCGATTCCGCCGCCGTGTGCTTCGATG
CCGTCCAAGCCGCCAAAGCGCGCGGCATCGACATTGTGCTGGCCGACACCGCCGGCCGCCTGCCCACGCAGCTTCACTTG
ATGGAAGAAATCAAAAAAGTGAAACGCGTGCTGCAAAAAGCCATGCCCGACGCGCCACACGAAATCATCGTCGTGCTTGA
TGCCAATATCGGTCAAAACGCCGTCAACCAAGTCAAAGCCTTTGACGACGCATTGGGGCTGACCGGTTTAATTGTTACCA
AGCTAGACGGCACGGCAAAAGGCGGCATCCTCGCCGCGCTTGCCTCCGACCGCCCCGTCCCCGTCCGCTATATCGGCGTG
GGCGAAGGCATAGACGATTTGCGCCCGTTTGACGCGCGCGCGTTTGTGGACGCACTGCTGGATTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(221-420)

SRP54-type protein, helical bundle domain (SRP54_N)

(136-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

94.537

100

0.945

Multiple sequence alignment