Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   amyaer_RS06305 Genome accession   NZ_CP012375
Coordinates   1317485..1319497 (-) Length   670 a.a.
NCBI ID   WP_066029671.1    Uniprot ID   -
Organism   Microcystis aeruginosa NIES-2481     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1312485..1324497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  amyaer_RS06295 (amyaer_1296) - 1314922..1316136 (-) 1215 WP_046661440.1 type II secretion system F family protein -
  amyaer_RS06300 (amyaer_1297) pilT 1316257..1317363 (-) 1107 WP_046661441.1 type IV pilus twitching motility protein PilT Machinery gene
  amyaer_RS06305 (amyaer_1298) pilB/pilB1 1317485..1319497 (-) 2013 WP_066029671.1 GspE/PulE family protein Machinery gene
  amyaer_RS06310 (amyaer_1299) grpE 1319738..1320460 (+) 723 WP_046661443.1 nucleotide exchange factor GrpE -
  amyaer_RS06315 (amyaer_1300) - 1320640..1321299 (-) 660 WP_046661444.1 HAD-IA family hydrolase -
  amyaer_RS06320 (amyaer_1301) - 1321390..1323354 (-) 1965 WP_046661445.1 amylo-alpha-1,6-glucosidase -
  amyaer_RS06325 (amyaer_1302) rpmH 1323445..1323582 (+) 138 WP_002739276.1 50S ribosomal protein L34 -
  amyaer_RS06330 (amyaer_1303) rnpA 1323609..1323971 (+) 363 WP_002763101.1 ribonuclease P protein component -
  amyaer_RS06335 (amyaer_1304) - 1323961..1324356 (+) 396 WP_002784373.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75099.16 Da        Isoelectric Point: 5.5769

>NTDB_id=153938 amyaer_RS06305 WP_066029671.1 1317485..1319497(-) (pilB/pilB1) [Microcystis aeruginosa NIES-2481]
MTFSAKKTRAVALRNYFSPFGNKLVQSGYIGAEEMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESLDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPASILIAMVDPDNLEATDDINRILRPRELGLQRLV
ITGEDYERLLEKFHWAQPELEKEKARLEKEKELEKLSDLTDIVASIEVVSGADDEVADDLGEGDANQAPVINLVNKILAK
ALQEGTSDIHIEPQEEFLKIRFRKDGVLHQAFDPLPKRITPAVTARFKIMADLDIAERRLPQDGKIRRIYQGRKVDFRVN
TLPSRYGEKVCLRILDNSSTQLGLDKLITDQKTLQIVRDLASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTAE
DPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDE
MGVETFMISGSLLGVLAQRLMRRVCTECRVAYHPTQEELARFGLSAANEQEVTFYKANTLTIEEIQQARREGNLCVKCNG
SGYKGRIGVYEVMQNSERLQQLINQGATTDRIKEAAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFSDSGLEAELKAKR
KSGLICSGCRAELKPEWLDCPYCMRPRFSG

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=153938 amyaer_RS06305 WP_066029671.1 1317485..1319497(-) (pilB/pilB1) [Microcystis aeruginosa NIES-2481]
ATGACATTTTCTGCCAAGAAAACCCGTGCCGTTGCCCTACGCAATTACTTTTCCCCCTTTGGTAACAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGAGGAAATGCAACAGGCCCTGGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AAAAACTAACCGGGCGACCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCGCTCGACCCCGAATTGAGCGATGTGGATGGACTAGAAATAGCCAGATTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGTCGAATCGAAGGAGAGCCGGCTTCAATTTTGATAGCCATGG
TCGATCCCGACAACCTAGAGGCTACGGACGATATTAATCGTATCCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTAGTG
ATTACCGGCGAAGATTACGAAAGATTACTGGAAAAATTCCACTGGGCCCAACCGGAACTAGAAAAGGAAAAAGCCAGACT
CGAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTCACCGATATTGTCGCCAGTATTGAGGTGGTCTCTGGTGCTGACG
ATGAAGTGGCCGATGATCTCGGAGAAGGAGACGCTAATCAAGCCCCCGTCATCAACCTGGTCAATAAAATACTAGCTAAA
GCTCTACAGGAAGGGACTTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGGAGT
TCTTCATCAAGCTTTTGATCCACTGCCGAAAAGAATTACCCCAGCGGTTACAGCCCGTTTTAAAATCATGGCCGATCTCG
ATATCGCTGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATTTATCAAGGGCGGAAAGTGGATTTTCGGGTTAAT
ACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGATAACTCCTCTACCCAGTTGGGTTTAGATAAACT
AATCACCGATCAAAAGACCCTGCAAATTGTCCGAGACTTGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCCCGA
CCGGTTCGGGGAAATCCACCACTTTATACTCGGTATTAGCGGAAAGAAATCACCCCGGAGTTAATATCAGTACCGCCGAA
GATCCGATCGAATATTCTCTACCAGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGATTCT
GCGGGCATTCATGCGACAAGATCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAAACAGCTAAAACAGCGATCG
AGGCCGCCTTAACCGGTCACTTGGTCTTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGCTCGTCTCGATGAG
ATGGGGGTTGAAACGTTTATGATTTCTGGTTCTCTGTTGGGAGTTTTGGCCCAGCGTCTGATGCGGCGCGTCTGTACCGA
GTGTCGAGTCGCCTATCACCCCACCCAAGAGGAATTGGCCCGTTTTGGTCTTTCCGCCGCCAATGAACAGGAAGTGACCT
TTTATAAGGCTAATACCCTGACCATAGAGGAAATTCAACAGGCGCGAAGAGAGGGCAATCTCTGCGTCAAGTGCAACGGC
AGCGGTTATAAAGGTCGCATCGGTGTCTATGAGGTAATGCAGAATAGTGAACGACTACAGCAATTAATTAACCAAGGGGC
AACCACCGATCGCATTAAGGAGGCGGCCGTCGATGAGGGCATGGTGACACTCCTAGCCTACAGTTTAAATCTGGTGCGCG
AGGGTTACACCACCCTCGAAGAAGTGGAACGGGTGACATTTAGCGATTCTGGTCTGGAAGCGGAATTAAAAGCTAAACGG
AAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCCGAACTAAAACCAGAATGGCTCGATTGTCCCTATTGTATGAGACCGCG
ATTTAGTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

74.018

98.806

0.731

Multiple sequence alignment