Detailed information NTG0153774

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AKN93_RS04315
• Genome accession: NZ_CP012360
• Coordinates: 882816..883715 (+)
• Length: 299 a.a.
• NCBI ID: WP_053105046.1
• Uniprot ID: -
• Organism: Thiopseudomonas alkaliphila strain C6918
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 877816..888715

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AKN93_RS04290 (AKN93_04300)	-	877935..878351 (+)	417	WP_053103338.1	PACE efflux transporter	-
AKN93_RS04295 (AKN93_04305)	elbB	878394..879047 (+)	654	WP_053105042.1	isoprenoid biosynthesis glyoxalase ElbB	-
AKN93_RS04300 (AKN93_04310)	-	879118..879627 (-)	510	WP_053105043.1	pilin	-
AKN93_RS04305 (AKN93_04315)	pilB	879898..881589 (+)	1692	WP_053105044.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AKN93_RS04310 (AKN93_04320)	pilC	881595..882812 (+)	1218	WP_053105045.1	type II secretion system F family protein	Machinery gene
AKN93_RS04315 (AKN93_04325)	pilD	882816..883715 (+)	900	WP_053105046.1	prepilin peptidase	Machinery gene
AKN93_RS04320 (AKN93_04330)	-	883923..884204 (-)	282	WP_053105047.1	GIY-YIG nuclease family protein	-
AKN93_RS04325 (AKN93_04335)	-	884469..885458 (-)	990	WP_053105048.1	hypothetical protein	-
AKN93_RS04330 (AKN93_04340)	-	885625..886836 (-)	1212	WP_053105049.1	NAD(P)/FAD-dependent oxidoreductase	-
AKN93_RS04335 (AKN93_04345)	-	886833..887291 (-)	459	WP_053103346.1	YiiD C-terminal domain-containing protein	-

Sequence

Protein

• Length: 299 a.a.
• Molecular weight: 32758.30 Da
• Isoelectric Point: 8.1041

>NTDB_id=153774 AKN93_RS04315 WP_053105046.1 882816..883715(+) (pilD) [Thiopseudomonas alkaliphila strain C6918]
MSDLLGLLQQELALFIALASLLGLLAGSFINVVVYRLPKMLQADWQQQAKVILAEAQPAVAARPEPDSTSPRFNLLVPRS
ACPRCQHVIKPWENIPLLSWLALKGKCSGCQQIISKRYPLVELASGILSAVVAWRFGFGLPAMAILLLTWGLLTMSLIDA
DHQLLPDILVLPLLWLGLLLNTQSMFVSLSDAVLGVAIGYLSLWSIYWLFKLIMGKEGMGYGDFKLLAMLGAWGGWQVLL
LTILLSSLVGAILGIIILRLQKEGIEKPIPYGPYLAVAGWIALLWGNQITQAYLEFAGL

Nucleotide

• Length: 900 bp

>NTDB_id=153774 AKN93_RS04315 WP_053105046.1 882816..883715(+) (pilD) [Thiopseudomonas alkaliphila strain C6918]
ATGAGTGATTTGCTTGGGTTACTACAGCAAGAGTTAGCTTTATTTATAGCTCTGGCTTCACTTTTAGGGTTATTAGCTGG
CAGCTTTATCAATGTGGTGGTATATCGCCTACCAAAAATGCTGCAGGCCGACTGGCAACAACAAGCTAAAGTAATTCTAG
CCGAAGCCCAGCCAGCGGTAGCTGCACGCCCAGAGCCTGATTCTACGAGTCCACGTTTTAACTTATTGGTACCACGCTCA
GCCTGCCCCCGTTGCCAGCATGTGATTAAGCCTTGGGAGAATATTCCGTTGCTTAGTTGGCTAGCGCTGAAAGGTAAGTG
CTCAGGCTGTCAGCAAATTATTAGTAAGCGCTATCCCTTGGTGGAGCTGGCCAGCGGCATTTTATCGGCAGTAGTGGCCT
GGAGATTTGGCTTTGGCCTACCAGCTATGGCTATATTGCTATTGACCTGGGGGCTACTGACGATGAGCCTGATTGACGCT
GATCATCAACTGCTTCCGGATATTTTAGTGTTGCCGTTACTTTGGCTGGGCTTGCTGCTAAATACCCAGTCTATGTTTGT
CAGCTTAAGTGATGCGGTATTGGGAGTAGCTATCGGTTATCTAAGTCTATGGAGTATTTATTGGCTATTTAAGCTAATTA
TGGGCAAAGAAGGCATGGGTTACGGTGACTTTAAGTTGCTGGCGATGCTGGGCGCTTGGGGTGGCTGGCAAGTCTTATTG
CTAACAATTTTACTGTCTTCGCTGGTGGGCGCAATTTTAGGCATTATTATTTTGCGCTTACAAAAAGAAGGCATAGAAAA
GCCTATTCCCTATGGCCCTTACCTCGCCGTCGCCGGATGGATCGCCTTACTATGGGGCAACCAAATCACCCAAGCTTATT
TGGAGTTTGCGGGGCTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-136)

• Type IV leader peptidase family (Peptidase_A24) (147-255)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.035	95.318	0.515
pilD	Acinetobacter baumannii D1279779	49.492	98.662	0.488
pilD	Acinetobacter nosocomialis M2	48.814	98.662	0.482
pilD	Vibrio campbellii strain DS40M4	50.709	94.314	0.478
pilD	Neisseria gonorrhoeae MS11	45.126	92.642	0.418