Detailed information NTG0153752

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AKN94_RS06560
• Genome accession: NZ_CP012359
• Coordinates: 1377724..1378623 (-)
• Length: 299 a.a.
• NCBI ID: WP_053105046.1
• Uniprot ID: -
• Organism: Thiopseudomonas alkaliphila strain C6819
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1372724..1383623

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AKN94_RS06540 (AKN94_06535)	-	1374148..1374606 (+)	459	WP_053103346.1	YiiD C-terminal domain-containing protein	-
AKN94_RS06545 (AKN94_06540)	-	1374603..1375814 (+)	1212	WP_053105049.1	NAD(P)/FAD-dependent oxidoreductase	-
AKN94_RS06550 (AKN94_06545)	-	1375981..1376970 (+)	990	WP_053105048.1	hypothetical protein	-
AKN94_RS06555 (AKN94_06550)	-	1377235..1377516 (+)	282	WP_053105047.1	GIY-YIG nuclease family protein	-
AKN94_RS06560 (AKN94_06555)	pilD	1377724..1378623 (-)	900	WP_053105046.1	prepilin peptidase	Machinery gene
AKN94_RS06565 (AKN94_06560)	pilC	1378627..1379844 (-)	1218	WP_053105045.1	type II secretion system F family protein	Machinery gene
AKN94_RS06570 (AKN94_06565)	pilB	1379850..1381537 (-)	1688	Protein_1286	type IV-A pilus assembly ATPase PilB	-
AKN94_RS06575 (AKN94_06570)	-	1381808..1382317 (+)	510	WP_053105043.1	pilin	-
AKN94_RS06580 (AKN94_06575)	elbB	1382388..1383041 (-)	654	WP_053105042.1	isoprenoid biosynthesis glyoxalase ElbB	-
AKN94_RS06585 (AKN94_06580)	-	1383084..1383500 (-)	417	WP_053103338.1	PACE efflux transporter	-

Sequence

Protein

• Length: 299 a.a.
• Molecular weight: 32758.30 Da
• Isoelectric Point: 8.1041

>NTDB_id=153752 AKN94_RS06560 WP_053105046.1 1377724..1378623(-) (pilD) [Thiopseudomonas alkaliphila strain C6819]
MSDLLGLLQQELALFIALASLLGLLAGSFINVVVYRLPKMLQADWQQQAKVILAEAQPAVAARPEPDSTSPRFNLLVPRS
ACPRCQHVIKPWENIPLLSWLALKGKCSGCQQIISKRYPLVELASGILSAVVAWRFGFGLPAMAILLLTWGLLTMSLIDA
DHQLLPDILVLPLLWLGLLLNTQSMFVSLSDAVLGVAIGYLSLWSIYWLFKLIMGKEGMGYGDFKLLAMLGAWGGWQVLL
LTILLSSLVGAILGIIILRLQKEGIEKPIPYGPYLAVAGWIALLWGNQITQAYLEFAGL

Nucleotide

• Length: 900 bp

>NTDB_id=153752 AKN94_RS06560 WP_053105046.1 1377724..1378623(-) (pilD) [Thiopseudomonas alkaliphila strain C6819]
ATGAGTGATTTGCTTGGGTTACTACAGCAAGAGTTAGCTTTATTTATAGCTCTGGCTTCACTTTTAGGGTTATTAGCTGG
CAGCTTTATCAATGTGGTGGTATATCGCCTACCAAAAATGCTGCAGGCCGACTGGCAACAACAAGCTAAAGTAATTCTAG
CCGAAGCCCAGCCAGCGGTAGCTGCACGCCCAGAGCCTGATTCTACGAGTCCACGTTTTAACTTATTGGTACCACGCTCA
GCCTGCCCCCGTTGCCAGCATGTGATTAAGCCTTGGGAGAATATTCCGTTGCTTAGTTGGCTAGCGCTGAAAGGTAAGTG
CTCAGGCTGTCAGCAAATTATTAGTAAGCGCTATCCCTTGGTGGAGCTGGCCAGCGGCATTTTATCGGCAGTAGTGGCCT
GGAGATTTGGCTTTGGCCTACCAGCTATGGCTATATTGCTATTGACCTGGGGGCTACTGACGATGAGCCTGATTGACGCT
GATCATCAACTGCTTCCGGATATTTTAGTGTTGCCGTTACTTTGGCTGGGCTTGCTGCTAAATACCCAGTCTATGTTTGT
CAGCTTAAGTGATGCGGTATTGGGAGTAGCTATCGGTTATCTAAGTCTATGGAGTATTTATTGGCTATTTAAGCTAATTA
TGGGCAAAGAAGGCATGGGTTACGGTGACTTTAAGTTGCTGGCGATGCTGGGCGCTTGGGGTGGCTGGCAAGTCTTATTG
CTAACAATTTTACTGTCTTCGCTGGTGGGCGCAATTTTAGGCATTATTATTTTGCGCTTACAAAAAGAAGGCATAGAAAA
GCCTATTCCCTATGGCCCTTACCTCGCCGTCGCCGGATGGATCGCCTTACTATGGGGCAACCAAATCACCCAAGCTTATT
TGGAGTTTGCGGGGCTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-136)

• Type IV leader peptidase family (Peptidase_A24) (147-255)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.035	95.318	0.515
pilD	Acinetobacter baumannii D1279779	49.492	98.662	0.488
pilD	Acinetobacter nosocomialis M2	48.814	98.662	0.482
pilD	Vibrio campbellii strain DS40M4	50.709	94.314	0.478
pilD	Neisseria gonorrhoeae MS11	45.126	92.642	0.418