Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AFK25_RS13265 Genome accession   NZ_CP012152
Coordinates   2521029..2522369 (-) Length   446 a.a.
NCBI ID   WP_035066129.1    Uniprot ID   A0AAW7TIS1
Organism   Anoxybacillus gonensis strain G2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2516029..2527369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFK25_RS13230 (AFK25_13250) - 2516621..2517100 (-) 480 WP_019417512.1 flagellar protein FlgN -
  AFK25_RS13235 (AFK25_13255) flgM 2517124..2517393 (-) 270 WP_019417513.1 flagellar biosynthesis anti-sigma factor FlgM -
  AFK25_RS13240 (AFK25_13260) - 2517484..2517891 (-) 408 WP_019417514.1 TIGR03826 family flagellar region protein -
  AFK25_RS13245 (AFK25_13265) - 2517914..2518351 (-) 438 WP_035066135.1 YaaR family protein -
  AFK25_RS13250 (AFK25_13270) - 2518363..2518587 (-) 225 WP_128713021.1 flagellar biosynthesis protein FlhB -
  AFK25_RS13255 (AFK25_13275) - 2518584..2520308 (-) 1725 WP_128713020.1 hypothetical protein -
  AFK25_RS13260 (AFK25_13280) - 2520363..2521032 (-) 670 Protein_2604 ComF family protein -
  AFK25_RS13265 (AFK25_13285) comFA 2521029..2522369 (-) 1341 WP_035066129.1 DEAD/DEAH box helicase Machinery gene
  AFK25_RS13270 (AFK25_13290) - 2522436..2523278 (-) 843 WP_019417520.1 DegV family protein -
  AFK25_RS13275 (AFK25_13295) - 2523377..2524057 (-) 681 WP_035066126.1 response regulator -
  AFK25_RS13280 (AFK25_13300) - 2524057..2525217 (-) 1161 WP_035066123.1 sensor histidine kinase -
  AFK25_RS13285 (AFK25_13305) - 2525411..2526049 (+) 639 WP_035066122.1 YigZ family protein -
  AFK25_RS13290 (AFK25_13310) - 2526144..2527163 (+) 1020 WP_035066119.1 LCP family protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 50357.59 Da        Isoelectric Point: 9.7025

>NTDB_id=151930 AFK25_RS13265 WP_035066129.1 2521029..2522369(-) (comFA) [Anoxybacillus gonensis strain G2]
MISLHLAGKQLLKSELSFSHDAKHVQIVDSIVKTKKGYRCVRCDNEDQQLFASFPCARCGRICTYCRKCVMMGRCSECNP
LVIWTGPIPSTSFSSPLKWSGKLSPAQQLASDEVCQAIDRNDSLLVWAVCGAGKTEVLFAGINLALSRGKRVCIAAPRTD
VVLELAPRLASVFPSVDMAALYGGSEDRGKLASLTVATTHQLLRYYRAFDVMIVDEVDAFPYSVDPMLSYAVERARKPTS
SLIYLTATPNEQWQYEVKSGKRKAVTIPARYHRHPLPVPTFEWCGNWQKKWRHHRLPLCVVQWMYARLEQKKQAFVFVPH
IDLLQQVVPYLQQFHPHIEGVHAEDPKRKEKVLTFRRGQIPILVTTTILERGVTVPNVDVAVLGAEQPIFTESALVQIAG
RVGRSAQYPTGDIRFFHFGKTKAMLKAKRHIVRMNEEARKRGLIDE

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=151930 AFK25_RS13265 WP_035066129.1 2521029..2522369(-) (comFA) [Anoxybacillus gonensis strain G2]
GTGATTTCGTTGCATCTCGCAGGCAAGCAGCTGCTAAAGTCTGAACTTTCTTTTTCTCATGATGCGAAACATGTTCAAAT
CGTTGACAGTATCGTCAAAACGAAAAAAGGTTATCGCTGTGTGCGATGCGACAATGAGGATCAACAACTTTTTGCCTCTT
TTCCGTGCGCTCGGTGCGGACGCATATGTACGTATTGTCGAAAATGTGTCATGATGGGACGATGTAGCGAATGCAATCCG
CTCGTCATATGGACGGGCCCAATTCCCTCCACCTCTTTTTCTTCACCGCTGAAATGGAGCGGTAAACTATCCCCAGCCCA
ACAACTTGCTTCCGACGAAGTGTGTCAGGCGATTGATCGAAACGACTCTTTGCTCGTTTGGGCCGTATGTGGTGCAGGAA
AAACGGAAGTGTTATTTGCGGGAATCAATCTGGCGCTGTCGCGCGGAAAGCGCGTTTGCATCGCCGCTCCGCGCACAGAT
GTCGTATTAGAGCTAGCTCCGCGCTTGGCGAGCGTATTTCCATCGGTCGATATGGCGGCATTATATGGCGGTAGCGAAGA
CCGTGGTAAACTTGCTTCATTGACGGTGGCAACGACGCATCAACTACTTCGGTATTATCGGGCGTTCGATGTCATGATTG
TTGATGAAGTGGATGCATTTCCGTATAGCGTTGATCCGATGCTTTCGTATGCCGTTGAACGTGCTCGAAAACCGACGTCT
TCACTCATTTACTTAACCGCAACACCTAACGAACAATGGCAATATGAAGTAAAAAGTGGAAAGCGAAAAGCGGTAACCAT
TCCTGCGCGCTATCATCGTCACCCACTCCCTGTCCCTACATTTGAATGGTGCGGCAATTGGCAAAAAAAGTGGCGTCACC
ACCGTTTACCTTTATGTGTCGTTCAATGGATGTATGCCCGTTTAGAACAAAAGAAGCAAGCTTTTGTATTCGTTCCCCAC
ATCGACTTGTTACAACAAGTTGTTCCGTACTTGCAACAATTTCATCCGCACATTGAAGGGGTGCATGCCGAAGATCCGAA
ACGAAAAGAAAAAGTGCTCACTTTTCGGCGCGGGCAAATTCCTATTCTCGTGACAACGACCATTTTAGAGCGCGGGGTGA
CTGTGCCCAACGTTGATGTCGCTGTGTTAGGTGCAGAACAACCGATTTTTACCGAAAGTGCGCTCGTGCAAATTGCTGGA
CGGGTCGGGCGGAGTGCCCAATATCCGACAGGTGATATTCGCTTTTTTCATTTTGGAAAAACGAAAGCAATGCTTAAAGC
AAAGCGCCATATTGTACGTATGAACGAAGAAGCGCGAAAGAGAGGATTGATCGACGAATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(305-405)

Type III restriction enzyme, res subunit (ResIII)

(102-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.81

99.103

0.513
  comFA Latilactobacillus sakei subsp. sakei 23K

37.556

100

0.379
  comFA/cflA Streptococcus pneumoniae Rx1

37.188

98.879

0.368
  comFA/cflA Streptococcus pneumoniae D39

37.188

98.879

0.368
  comFA/cflA Streptococcus pneumoniae R6

37.188

98.879

0.368
  comFA/cflA Streptococcus pneumoniae TIGR4

36.961

98.879

0.365

Multiple sequence alignment