Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AFK25_RS10015 Genome accession   NZ_CP012152
Coordinates   1903916..1904986 (-) Length   356 a.a.
NCBI ID   WP_026011547.1    Uniprot ID   A0AAW7TD74
Organism   Anoxybacillus gonensis strain G2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1898916..1909986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFK25_RS09970 (AFK25_09990) - 1899543..1900313 (+) 771 WP_035066773.1 YqhG family protein -
  AFK25_RS09975 (AFK25_09995) - 1900328..1900516 (-) 189 WP_019416934.1 YqzE family protein -
  AFK25_RS09980 (AFK25_10000) - 1900543..1901049 (-) 507 WP_035066770.1 shikimate kinase -
  AFK25_RS09985 (AFK25_10005) comGG 1901063..1901479 (-) 417 WP_009373938.1 competence type IV pilus minor pilin ComGG -
  AFK25_RS09990 (AFK25_10010) comGF 1901439..1901873 (-) 435 WP_035066767.1 competence type IV pilus minor pilin ComGF -
  AFK25_RS09995 (AFK25_10015) comGE 1901870..1902178 (-) 309 WP_035066764.1 competence type IV pilus minor pilin ComGE -
  AFK25_RS10000 (AFK25_10020) comGD 1902162..1902599 (-) 438 WP_009373943.1 competence type IV pilus minor pilin ComGD -
  AFK25_RS10005 (AFK25_10025) comGC 1902592..1902894 (-) 303 WP_019416931.1 competence type IV pilus major pilin ComGC Machinery gene
  AFK25_RS10010 (AFK25_10030) comGB 1902901..1903932 (-) 1032 WP_035066761.1 competence type IV pilus assembly protein ComGB -
  AFK25_RS10015 (AFK25_10035) comGA 1903916..1904986 (-) 1071 WP_026011547.1 competence type IV pilus ATPase ComGA Machinery gene
  AFK25_RS10020 (AFK25_10040) - 1905098..1905469 (-) 372 WP_009373947.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AFK25_RS10025 (AFK25_10045) - 1905657..1906355 (+) 699 WP_035066758.1 helix-turn-helix transcriptional regulator -
  AFK25_RS10030 (AFK25_10050) - 1906425..1906667 (+) 243 WP_009373952.1 DUF2626 domain-containing protein -
  AFK25_RS10035 (AFK25_10055) - 1906696..1907799 (-) 1104 WP_049720895.1 class I SAM-dependent methyltransferase -
  AFK25_RS10040 (AFK25_10060) - 1907915..1908547 (-) 633 WP_009373954.1 MBL fold metallo-hydrolase -
  AFK25_RS10045 (AFK25_10065) - 1908679..1908864 (+) 186 WP_009373955.1 DUF2759 domain-containing protein -
  AFK25_RS10050 (AFK25_10070) - 1908891..1909760 (-) 870 WP_026011545.1 M14 family metallopeptidase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40037.83 Da        Isoelectric Point: 8.6787

>NTDB_id=151917 AFK25_RS10015 WP_026011547.1 1903916..1904986(-) (comGA) [Anoxybacillus gonensis strain G2]
MQAIERLADRLIEEAYMLQASDIHIVPRKQDALVQFRLGGMLVTKNVLSKQTCERLLAHFKFLADMDIGERRRPQSGAME
MNLTHTTIHLRLSTLPTIYDESLVIRLLPLHSFLPLKQLALFPSTVKKLLALLNYSHGLIIFTGPTGSGKTTTLYSLLNV
CRHHFQRNVITLEDPVEKRAEDVLQVQVNEKAGITYAAGLKAILRHDPDVIMVGEIRDAETARIAVRASLTGHLVLTTMH
TKDAVGAIYRLLEFGVPFQEMAQTLVAVTAQRLVQLKCPFCEGECSPFCRQYRPVRRAAVYELLYGNELAQAMRAAKGEQ
ATYMYTRLKDVIKKGIALGFLHESTIESWLLDEAAL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=151917 AFK25_RS10015 WP_026011547.1 1903916..1904986(-) (comGA) [Anoxybacillus gonensis strain G2]
TTGCAGGCTATTGAACGGCTAGCTGATCGCCTGATTGAAGAAGCTTATATGCTTCAAGCTTCCGACATTCATATCGTTCC
ACGCAAACAAGATGCGCTTGTGCAATTTCGCTTAGGGGGAATGCTCGTTACAAAAAATGTGTTATCGAAACAGACATGTG
AACGTTTGCTTGCCCACTTTAAATTTCTCGCTGATATGGATATTGGAGAACGTCGTCGTCCGCAAAGCGGAGCGATGGAA
ATGAACCTCACCCACACGACGATACATCTTCGGTTATCAACGTTGCCTACCATCTATGATGAAAGCTTAGTTATCCGCTT
GCTTCCGTTACACTCATTTCTTCCGCTTAAACAACTCGCGCTCTTTCCTTCAACCGTAAAAAAGTTGCTCGCCTTATTAA
ACTATTCTCATGGGCTTATTATTTTCACAGGACCGACTGGATCTGGGAAAACGACAACGCTATACAGTTTGTTAAACGTA
TGTCGTCACCATTTTCAACGAAATGTCATTACGCTAGAAGATCCGGTTGAAAAGCGGGCGGAAGATGTATTGCAAGTGCA
AGTAAACGAAAAAGCGGGTATTACGTATGCGGCAGGGTTAAAAGCGATTCTCCGCCACGATCCAGATGTCATTATGGTTG
GGGAAATTCGTGATGCGGAGACTGCGCGAATTGCCGTTCGCGCCTCATTAACAGGACATCTCGTCTTAACAACTATGCAT
ACGAAAGATGCGGTTGGAGCGATTTATCGCCTTCTTGAGTTCGGTGTGCCATTTCAAGAAATGGCCCAAACGTTAGTCGC
TGTAACAGCGCAACGTCTTGTACAATTAAAATGTCCGTTTTGTGAAGGAGAGTGCTCACCTTTTTGTCGACAGTATCGAC
CTGTCCGGCGAGCAGCTGTTTATGAGCTGCTATATGGAAATGAACTGGCGCAAGCAATGAGAGCGGCGAAAGGAGAGCAG
GCGACATATATGTACACTCGATTAAAAGATGTGATTAAAAAAGGAATTGCACTCGGTTTTTTACATGAAAGCACCATTGA
GAGTTGGTTGTTAGATGAAGCGGCGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

60.169

99.438

0.598
  pilB Glaesserella parasuis strain SC1401

40.227

99.157

0.399

Multiple sequence alignment