Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AC241_RS21035 Genome accession   NZ_CP012099
Coordinates   4080664..4081707 (-) Length   347 a.a.
NCBI ID   WP_029443296.1    Uniprot ID   A0A9X6LTP0
Organism   Bacillus thuringiensis strain HS18-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4075664..4086707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AC241_RS20990 (AC241_21010) - 4076100..4076300 (-) 201 WP_000106083.1 YqzE family protein -
  AC241_RS20995 (AC241_21015) aroK 4076336..4076833 (-) 498 WP_050844517.1 shikimate kinase AroK -
  AC241_RS21000 (AC241_21020) - 4076949..4077599 (-) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  AC241_RS21005 (AC241_21025) comGG 4077774..4078145 (-) 372 WP_016080292.1 competence type IV pilus minor pilin ComGG -
  AC241_RS21010 (AC241_21030) comGF 4078142..4078612 (-) 471 WP_029443294.1 competence type IV pilus minor pilin ComGF -
  AC241_RS21015 (AC241_21035) - 4078582..4078884 (-) 303 WP_016080290.1 hypothetical protein -
  AC241_RS21020 (AC241_21040) comGD 4078877..4079332 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  AC241_RS21025 (AC241_21045) comGC 4079329..4079628 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  AC241_RS21030 (AC241_21050) comGB 4079640..4080677 (-) 1038 WP_050844873.1 competence type IV pilus assembly protein ComGB -
  AC241_RS21035 (AC241_21055) comGA 4080664..4081707 (-) 1044 WP_029443296.1 competence type IV pilus ATPase ComGA Machinery gene
  AC241_RS21040 (AC241_21060) - 4081913..4082608 (+) 696 WP_000434107.1 helix-turn-helix transcriptional regulator -
  AC241_RS21045 (AC241_21065) - 4082735..4082977 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  AC241_RS21050 (AC241_21070) - 4083085..4084479 (+) 1395 WP_029443297.1 L-cystine transporter -
  AC241_RS21055 (AC241_21075) - 4084559..4084951 (-) 393 WP_048563827.1 hypothetical protein -
  AC241_RS21060 (AC241_21080) - 4085039..4085254 (-) 216 WP_001008326.1 DUF3912 family protein -
  AC241_RS21065 (AC241_21085) - 4085508..4085819 (+) 312 WP_001093240.1 hypothetical protein -
  AC241_RS21070 (AC241_21090) - 4085854..4086342 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39178.54 Da        Isoelectric Point: 8.5432

>NTDB_id=151399 AC241_RS21035 WP_029443296.1 4080664..4081707(-) (comGA) [Bacillus thuringiensis strain HS18-1]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRHCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYEILYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=151399 AC241_RS21035 WP_029443296.1 4080664..4081707(-) (comGA) [Bacillus thuringiensis strain HS18-1]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAGGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACATTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGATCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGATACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.62

100

0.556
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment