Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ADJ80_RS04560 Genome accession   NZ_CP012067
Coordinates   918570..919124 (+) Length   184 a.a.
NCBI ID   WP_050693132.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus strain W10433     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913570..924124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ADJ80_RS04545 (ADJ80_04565) - 914547..915878 (-) 1332 WP_050693129.1 DUF4123 domain-containing protein -
  ADJ80_RS04550 (ADJ80_04570) ansB 916044..917093 (-) 1050 WP_050693130.1 L-asparaginase 2 -
  ADJ80_RS04555 (ADJ80_04575) - 917394..918548 (+) 1155 WP_050693131.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ADJ80_RS04560 (ADJ80_04580) pilF/pilF2 918570..919124 (+) 555 WP_050693132.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ADJ80_RS04565 (ADJ80_04585) - 919342..920415 (+) 1074 WP_050693133.1 RodZ domain-containing protein -
  ADJ80_RS04570 (ADJ80_04590) ispG 920426..921529 (+) 1104 WP_050693134.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ADJ80_RS04575 (ADJ80_04595) hisS 921554..922831 (+) 1278 WP_050693135.1 histidine--tRNA ligase -
  ADJ80_RS04580 (ADJ80_04600) - 922842..923456 (+) 615 WP_050693136.1 YfgM family protein -
  ADJ80_RS04585 (ADJ80_04605) - 923512..923964 (+) 453 WP_050693137.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20956.83 Da        Isoelectric Point: 7.2832

>NTDB_id=151028 ADJ80_RS04560 WP_050693132.1 918570..919124(+) (pilF/pilF2) [Aggregatibacter aphrophilus strain W10433]
MVNIQFIFYRFVPFIFPFLFSACVVHSDAPDFDKQAAAKARVELALGYLQQQDGSQAKLNLDKALSYAPKYYLVHAALGY
FYQQQGDVEHAKQSYLTAIKLDDKQGDVFNNFGAFLCSQGEYQGAYEQFKQALKSLNYYHQADTYENLALCALSAKDQKI
YQENLAALEKIEPARAKKLTQFVK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=151028 ADJ80_RS04560 WP_050693132.1 918570..919124(+) (pilF/pilF2) [Aggregatibacter aphrophilus strain W10433]
ATGGTCAATATTCAGTTTATTTTTTACCGTTTTGTACCGTTTATTTTTCCTTTTCTGTTTTCTGCCTGTGTTGTTCATTC
CGATGCACCGGATTTTGATAAACAAGCTGCAGCTAAAGCCCGTGTGGAGCTGGCATTGGGCTATTTACAGCAACAAGATG
GTAGCCAAGCCAAATTGAATTTGGATAAAGCCCTTTCTTATGCCCCGAAATATTATTTAGTGCACGCTGCGTTAGGGTAT
TTTTATCAGCAACAAGGGGATGTTGAACATGCCAAACAATCGTATTTAACGGCGATTAAACTGGATGATAAACAAGGGGA
TGTATTCAATAACTTCGGTGCGTTTCTTTGTTCGCAAGGGGAATATCAGGGCGCGTATGAACAATTTAAACAGGCATTAA
AAAGCCTGAATTATTATCATCAAGCTGACACCTATGAAAACCTTGCACTGTGTGCGTTATCGGCGAAAGATCAGAAAATT
TATCAAGAGAACCTTGCTGCTCTTGAAAAGATTGAACCGGCACGAGCCAAGAAATTAACTCAGTTTGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.229

90.217

0.516
  pilF Glaesserella parasuis strain SC1401

42.703

100

0.429

Multiple sequence alignment