Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BSM4216_RS04265 Genome accession   NZ_CP012024
Coordinates   904024..907794 (+) Length   1256 a.a.
NCBI ID   WP_048622852.1    Uniprot ID   -
Organism   Bacillus smithii strain DSM 4216     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 899024..912794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSM4216_RS04260 (BSM4216_1038) addB 900542..904024 (+) 3483 WP_048622851.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BSM4216_RS04265 (BSM4216_1039) addA 904024..907794 (+) 3771 WP_048622852.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BSM4216_RS04270 (BSM4216_1041) - 907882..908100 (-) 219 WP_003353726.1 spore germination protein -
  BSM4216_RS04275 (BSM4216_1042) - 908102..908518 (-) 417 WP_048622853.1 spore germination protein GerPE -
  BSM4216_RS04280 (BSM4216_1043) - 908526..908705 (-) 180 WP_003353724.1 hypothetical protein -
  BSM4216_RS04285 (BSM4216_1044) gerPC 908680..909300 (-) 621 WP_048622854.1 spore germination protein GerPC -
  BSM4216_RS04290 (BSM4216_1045) - 909316..909543 (-) 228 WP_048622855.1 spore germination protein GerPB -
  BSM4216_RS04295 (BSM4216_1046) - 909557..909778 (-) 222 WP_003353720.1 spore germination protein -
  BSM4216_RS04300 (BSM4216_1047) - 910019..911185 (+) 1167 WP_048622856.1 DUF418 domain-containing protein -
  BSM4216_RS04305 (BSM4216_1048) - 911213..912115 (+) 903 WP_048622857.1 fumarylacetoacetate hydrolase family protein -
  BSM4216_RS04310 (BSM4216_1049) - 912312..912695 (+) 384 WP_048624391.1 YisL family protein -

Sequence

Protein


Download         Length: 1256 a.a.        Molecular weight: 145639.14 Da        Isoelectric Point: 5.0512

>NTDB_id=150494 BSM4216_RS04265 WP_048622852.1 904024..907794(+) (addA) [Bacillus smithii strain DSM 4216]
MGKYPIPLKPDHVSWTDDQWKAIMADGQDILVAAAAGSGKTAVLVERIIQKITSRKNPVDVDELLVVTFTNASAAEMKHR
IGQALEEAANQQPNSEHLRKQLGLLNRASISTLHSFCLEVIQKFYYLIDIDPAFRIVENMEGTLLREEALDELFEEEYGK
EGNDRFYQLVETFSNDRSDAELQDLVNKLYFFSQSHPNPELWLEQLADMYDVNEEDSIDELPFLGALKFEIGLQLEIAFQ
LLREGLELTKRPGGPAPRAENFLIDLEMVESLLAAKDSWQDLYEKFQNVTFDRLKSCRGSEYDKTLVDESKNIRDQAKKI
IQKIKDDYFSRNPRYYLRDLKEMKDVVETLAYLVKAFMNRYQEKKREKGLVDFSDLEHYCLAILTESESNGERVPSEAAL
YYQHRFKEVLVDEYQDTNLVQEAILQLVSKRPETDGNLFMVGDVKQSIYRFRLAEPNLFLEKYRRFTADGEGSGLKIDLS
KNFRSRYEVLAGTNFLFKQIMGAKVGEIEYDEQAELVKGSSYPEEEEYPVEVVLMDQGEKQERAVSTEEYADQALFDEED
LEQSQIESRWMAGKIRELILERKPIFDPKTQTHRPIQYRDIVILLRSMTWAPEIMEEFKKAGIPVYADLSTGYFNATEIA
IMMSLLKVIDNPYQDIPLASVLRSPIVNCSEDELAKIRLHSKKGSYYDALKAFIQAVPKPSEEELHEKVSLFAKELPKWR
SKARTGSLSELIWQLYRDTGFYEFVGGLPGGKQRQANLRALYDRARQYESTSFRGLFRFLRFIERMNERGDDLGAARALS
EQEDVVRLMTIHASKGLEFPVVFVAGLGKQFNFEDLKKSYLMDKEYGFASSYVNVEKRISYPTIIQMAFKRKKRLESIAE
EMRVLYVALTRAKEKLYLIGTLKNAEKAISSWKKALSRPDWLLSEYDRANANCYLDWIGPALVRHRDACLLHEDETDSPS
EIQEIWHHPSKWLVHLYHKDEFKETVTNESQEENGWAELVEKGKIVPFESSFQKEITKQLEWKYPFADSVRLRSKQSVSE
LKRLFEMPDETAGTDLIRRHEKPIFERPKFMQEKKLTPAEIGTAMHAVMQHIPLSVFPTKESIIELVAQLRQKELITAEQ
AEAIHIEEILGFFETSVGKRLIRADRIWREVPFSMAIEAKELNEKWKDVADSVLVQGIIDCLFEENGQLVLLDYKTDRMD
RFVKGGKDVVQVLENRYRIQIALYTRAVEQILKRPLSEAYLYFFDGGRLIEINDRL

Nucleotide


Download         Length: 3771 bp        

>NTDB_id=150494 BSM4216_RS04265 WP_048622852.1 904024..907794(+) (addA) [Bacillus smithii strain DSM 4216]
ATGGGCAAATATCCAATTCCGTTAAAACCGGATCATGTAAGTTGGACAGACGATCAATGGAAAGCGATCATGGCGGACGG
ACAAGATATATTAGTGGCCGCAGCGGCAGGCTCTGGAAAAACGGCTGTCTTGGTGGAACGGATTATCCAAAAAATCACCA
GTCGCAAAAATCCGGTGGATGTGGATGAATTATTGGTGGTCACATTTACAAATGCTTCTGCTGCAGAAATGAAACATCGA
ATTGGACAGGCTTTAGAAGAAGCCGCCAATCAGCAGCCAAATTCAGAGCATTTGCGTAAACAATTAGGCCTTCTGAACCG
AGCGTCCATTTCCACTCTTCATTCTTTTTGCTTAGAAGTGATTCAAAAATTTTACTATTTGATCGATATCGATCCGGCGT
TTCGGATTGTTGAGAATATGGAAGGAACGCTTCTTCGAGAGGAAGCGCTCGATGAACTTTTTGAAGAAGAATACGGAAAA
GAAGGAAACGATCGCTTTTACCAATTGGTTGAAACTTTTTCCAACGACCGTAGCGATGCAGAACTTCAGGATTTAGTGAA
TAAGTTGTATTTTTTTTCACAATCTCATCCGAATCCGGAATTATGGTTGGAACAGTTAGCCGATATGTATGATGTGAATG
AAGAGGATTCCATAGATGAATTGCCGTTTTTAGGGGCACTGAAATTTGAAATTGGACTGCAGTTGGAAATTGCCTTCCAG
CTCCTTCGCGAGGGATTAGAGCTGACGAAGAGGCCGGGTGGTCCGGCTCCTCGGGCGGAAAACTTTTTGATTGATCTGGA
GATGGTCGAAAGCCTTTTAGCCGCAAAGGATTCTTGGCAAGATTTATACGAAAAGTTTCAAAACGTGACTTTTGATAGAT
TAAAAAGCTGCCGAGGATCAGAGTACGACAAAACCCTCGTGGATGAATCAAAAAACATACGGGATCAGGCGAAGAAAATC
ATACAAAAGATAAAAGACGACTATTTTTCAAGAAATCCTCGCTACTATTTACGGGATTTGAAAGAGATGAAAGACGTTGT
CGAAACATTGGCTTATTTAGTAAAAGCGTTTATGAACCGCTACCAAGAAAAAAAGAGAGAAAAAGGGCTGGTCGATTTCT
CCGACCTGGAACATTATTGCTTGGCGATTTTGACGGAATCGGAATCTAATGGCGAGCGAGTACCTTCGGAAGCCGCTTTG
TATTATCAACATCGATTCAAAGAAGTATTGGTGGATGAATATCAAGATACGAATCTAGTTCAAGAAGCCATTCTTCAACT
CGTTTCCAAGAGACCGGAGACAGACGGAAATTTGTTTATGGTTGGAGATGTGAAGCAGTCTATTTATCGTTTCCGACTGG
CAGAACCAAATCTGTTCTTAGAGAAATACCGCCGGTTTACGGCAGACGGGGAAGGAAGCGGGCTAAAAATCGACCTTTCG
AAAAATTTTCGCAGCCGGTATGAAGTCCTTGCAGGAACGAATTTTCTTTTTAAACAGATCATGGGTGCTAAAGTGGGAGA
AATTGAATACGATGAACAGGCGGAACTTGTTAAAGGTTCATCTTATCCTGAAGAGGAAGAATATCCGGTAGAAGTGGTCT
TGATGGACCAAGGAGAAAAACAGGAACGCGCTGTTTCGACCGAGGAATATGCGGATCAAGCTTTATTCGACGAAGAAGAT
TTGGAGCAATCGCAAATCGAGTCGCGATGGATGGCGGGGAAAATCCGGGAATTAATTTTGGAACGAAAACCTATTTTTGA
TCCGAAAACGCAAACTCATCGCCCGATCCAATACCGGGATATCGTTATTTTATTGCGGTCCATGACGTGGGCGCCAGAGA
TCATGGAAGAATTTAAAAAGGCCGGAATACCCGTTTATGCCGATTTGTCTACTGGATATTTCAATGCGACGGAAATCGCT
ATTATGATGTCTCTTTTAAAAGTCATCGACAATCCTTATCAAGATATACCTCTTGCTTCCGTTCTTCGCTCGCCGATCGT
AAACTGTTCAGAAGATGAATTGGCAAAAATCCGCCTTCATTCTAAAAAAGGAAGCTATTATGATGCACTAAAAGCCTTTA
TTCAAGCGGTTCCGAAACCGTCTGAAGAAGAACTCCATGAAAAAGTGAGTTTATTTGCAAAAGAGCTTCCTAAATGGAGA
TCGAAGGCACGTACGGGAAGTCTTTCAGAACTGATCTGGCAGCTATATCGTGATACAGGATTTTATGAATTTGTGGGCGG
GCTCCCTGGAGGCAAACAGCGGCAAGCCAATTTAAGGGCATTATACGATCGCGCTCGCCAATATGAAAGCACATCGTTTC
GAGGGCTGTTCCGGTTTTTGCGCTTTATTGAACGGATGAATGAACGCGGGGACGATTTAGGAGCTGCACGAGCTCTTAGC
GAACAAGAAGATGTCGTTCGACTTATGACGATACATGCAAGCAAAGGACTGGAATTTCCTGTCGTCTTTGTGGCCGGTCT
TGGCAAACAGTTTAACTTCGAGGATCTTAAAAAATCGTATTTAATGGACAAAGAGTACGGTTTTGCCAGCAGCTATGTAA
ATGTTGAAAAAAGAATCAGTTATCCAACGATTATTCAAATGGCCTTTAAAAGAAAAAAAAGGTTGGAATCAATAGCTGAG
GAAATGCGGGTGCTATATGTTGCCTTAACCCGTGCGAAAGAAAAGTTATATTTAATAGGAACGTTAAAAAATGCCGAAAA
GGCCATATCTTCTTGGAAAAAGGCACTAAGCAGGCCAGACTGGTTATTGAGCGAATACGACAGGGCCAATGCCAATTGTT
ATTTGGATTGGATCGGTCCGGCGCTTGTCCGTCATAGAGACGCATGTTTACTTCATGAAGATGAAACGGACTCTCCTTCG
GAAATACAAGAAATTTGGCATCATCCTTCAAAATGGTTGGTCCATCTTTACCATAAAGATGAGTTTAAAGAAACAGTAAC
GAACGAGAGTCAAGAAGAAAACGGATGGGCTGAACTGGTGGAAAAAGGAAAAATCGTTCCATTTGAATCTTCATTTCAAA
AAGAGATTACAAAGCAATTGGAGTGGAAATATCCATTTGCTGATTCAGTGCGGCTTCGTTCTAAACAATCGGTTTCCGAA
CTGAAAAGATTATTTGAAATGCCTGACGAAACAGCTGGTACCGATCTTATTCGCCGACATGAAAAACCTATTTTTGAGCG
GCCGAAGTTTATGCAAGAAAAAAAATTAACACCCGCTGAAATAGGAACGGCCATGCATGCTGTCATGCAGCATATTCCGC
TAAGCGTTTTTCCAACGAAGGAATCGATCATTGAATTGGTTGCTCAGCTGCGACAGAAAGAATTGATTACCGCTGAACAA
GCGGAAGCGATTCATATTGAAGAAATTCTAGGTTTCTTTGAAACTTCTGTCGGAAAACGCCTTATCCGCGCTGATCGAAT
TTGGCGAGAGGTGCCATTTAGTATGGCGATAGAAGCGAAGGAATTGAACGAAAAATGGAAGGATGTAGCGGATTCCGTTT
TAGTTCAAGGCATCATTGATTGCTTATTCGAGGAGAATGGTCAGCTTGTTTTGTTGGATTATAAAACAGATCGAATGGAC
CGATTCGTAAAAGGCGGGAAGGATGTTGTTCAAGTATTGGAAAATCGCTATCGTATTCAAATAGCTTTATATACAAGAGC
GGTGGAGCAAATTTTAAAGCGGCCGCTTTCTGAAGCCTATTTATACTTTTTTGACGGTGGCCGTCTTATAGAGATAAACG
ACCGATTATGA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(15-466)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1073-1242)

UvrD-like helicase C-terminal domain (UvrD_C)

(477-901)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

55.92

99.522

0.557

Multiple sequence alignment