Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AXW93_RS27745 Genome accession   NZ_CP014866
Coordinates   5858086..5860305 (+) Length   739 a.a.
NCBI ID   WP_022579991.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA_154197     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5853086..5865305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW93_RS27725 (AXW93_26885) - 5854061..5855329 (-) 1269 WP_003103278.1 malic enzyme -
  AXW93_RS27730 (AXW93_26890) - 5855432..5856871 (-) 1440 WP_003095844.1 M48 family metalloprotease -
  AXW93_RS27735 (AXW93_26895) - 5856868..5857635 (-) 768 WP_024947550.1 thermonuclease family protein -
  AXW93_RS27740 (AXW93_26900) rpmE 5857695..5857910 (-) 216 WP_003095846.1 50S ribosomal protein L31 -
  AXW93_RS27745 (AXW93_26905) priA 5858086..5860305 (+) 2220 WP_022579991.1 primosomal protein N' Machinery gene
  AXW93_RS27750 (AXW93_26910) argS 5860554..5862317 (+) 1764 WP_003135774.1 arginine--tRNA ligase -
  AXW93_RS27755 (AXW93_26915) - 5862352..5863047 (+) 696 WP_003095850.1 SPOR domain-containing protein -
  AXW93_RS27760 (AXW93_26920) hslV 5863166..5863699 (+) 534 WP_003095852.1 ATP-dependent protease subunit HslV -
  AXW93_RS27765 (AXW93_26925) hslU 5863728..5865071 (+) 1344 WP_003095854.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82122.64 Da        Isoelectric Point: 8.7962

>NTDB_id=150494 AXW93_RS27745 WP_022579991.1 5858086..5860305(+) (priA) [Pseudomonas aeruginosa strain PA_154197]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEDKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHAAAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLEINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPLGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=150494 AXW93_RS27745 WP_022579991.1 5858086..5860305(+) (priA) [Pseudomonas aeruginosa strain PA_154197]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGGTGA
CGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCGCCGCGCAATCCAGCCTCGACGATCCGC
GCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTTTCCCATGAGTTGCTG
AACCAATTGGAAATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TTCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGCCCGCAGACCCTGGC
GCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAGCGCCTCGATGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGCTCGGCACTGTTCACGCCGCTGAAGCGGCCGGGACTG
ATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGCCTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCGCGCCTGGAGAATGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGTCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGT
ATTCCTCAATCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAGTGCGGCAAGCTCGACCTGCGTCCGCTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGGCGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGATGGGCGGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCACCCATGGAGCGGCGTGCCGGCAAGCACC
GCGCGCAACTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACACCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.619

99.459

0.474

Multiple sequence alignment