Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BSM4216_RS04260 Genome accession   NZ_CP012024
Coordinates   900542..904024 (+) Length   1160 a.a.
NCBI ID   WP_048622851.1    Uniprot ID   -
Organism   Bacillus smithii strain DSM 4216     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 895542..909024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSM4216_RS04235 (BSM4216_1031) - 896024..896416 (-) 393 WP_048622849.1 hypothetical protein -
  BSM4216_RS04240 (BSM4216_1032) - 896670..897293 (+) 624 WP_003353731.1 TVP38/TMEM64 family protein -
  BSM4216_RS04245 (BSM4216_1034) - 897426..898157 (+) 732 WP_048622850.1 FUSC family protein -
  BSM4216_RS04250 (BSM4216_1035) - 898442..898924 (-) 483 WP_156179199.1 hypothetical protein -
  BSM4216_RS04255 (BSM4216_1036) - 899022..900026 (-) 1005 WP_156179197.1 transposase -
  BSM4216_RS04260 (BSM4216_1038) addB 900542..904024 (+) 3483 WP_048622851.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BSM4216_RS04265 (BSM4216_1039) addA 904024..907794 (+) 3771 WP_048622852.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BSM4216_RS04270 (BSM4216_1041) - 907882..908100 (-) 219 WP_003353726.1 spore germination protein -
  BSM4216_RS04275 (BSM4216_1042) - 908102..908518 (-) 417 WP_048622853.1 spore germination protein GerPE -
  BSM4216_RS04280 (BSM4216_1043) - 908526..908705 (-) 180 WP_003353724.1 hypothetical protein -

Sequence

Protein


Download         Length: 1160 a.a.        Molecular weight: 134531.41 Da        Isoelectric Point: 6.1410

>NTDB_id=150493 BSM4216_RS04260 WP_048622851.1 900542..904024(+) (addB) [Bacillus smithii strain DSM 4216]
MSVRFILGRSGTGKTKFIIDEITHQLIENPSGKPIIYIVPDQMTFLSEYTLVNTPGLNGMMRAQVYSFSRLAWKVLQETG
GFARIHLSSAGLNMLIRKIIEERKENLRLFAKAADKSGFIQHVEKALIELKRYCVGTDELAARRTEFEENGEAQLLIDKL
HDLHLIYEEFENHLVDRYIASEDYFQLLAESIQKSEYLQDAQIYIDGFHSFTPQEYLVIYELMKAASQVSIALTLDRPYS
EELPRETDLFRMTGETYASLWKMARDSGISTENIMMTEFVRSKEQSLIHLERFFDKRPAKPFIGEGAPVVFEAANRRAEI
EGVAREIRRLVREHHHRYKEIAVLVRNGQDYQELIETIFYDYEIPFFIDQKRTMLNHPFIELIRSSLEILTSFWRYEPVF
RAVKTELLYPFKMKAEQIREKIDRFENYCLAHGVKGELWTTKKRWVYRRYRGLELTDAPQTDEERAIEEEINELRAMIRT
PVLRLDRRFRQAKNGRDFCEALYKYFEELDIPAKLERLSMEAEENGDLLAARDHEQAWNAIIGLLDEFVEMLGDQPFTLK
KFATILEAGIESMRFTNVPPSADQVIIADLELSRLSDIKTAFIVGVNDGVLPAKMAEEGILTDEERERMASLGMKLAPTS
KTRLLNEEFIAYKAFTTPSEQLYLSYPIANEEGKALLPSLYIKRMKEIYPNIKEQLLLNDPSELEEQDQLSYISHPGTAI
SYLTSQIQLKLRNYPMYSIWWDVYNFYMSHQDWKWKARRILSSLFYRNEAKNLTEETSKELYGDHILASVSRMELFHGCP
FSHFARFGLGLREREVFRLEAPDIGELFHGALKWMADEVKKRGLGWKTLTKDQCAQLARESIQYLAPKLQNQILLSSNRY
HYIKKKLESIISKASYILSEHSRASGFEPIGLELGFGPKAELPPFTFTLKNGIKMELAGRIDRVDKAEENSSVFLRVIDY
KSSSKDLDLTEVYYGLALQMLTYLDIVLANSEQLIGTPALPAGVLYFHVHNPMLKTNKMLTMDEVEEEIFKQYKMKGLVL
SDPNVVRLMDQTLETGDSAIISAGLKKDGTLNARSKAASDNDFESLRKYVRKFYEKTGNDIVSGKIDIAPYKYKKKTPCE
FCSFRSLCQFDPSFEENRYRIIIPLKDEDVLKLIRERGEQ

Nucleotide


Download         Length: 3483 bp        

>NTDB_id=150493 BSM4216_RS04260 WP_048622851.1 900542..904024(+) (addB) [Bacillus smithii strain DSM 4216]
ATGTCTGTTCGTTTTATTTTAGGACGTTCCGGAACCGGAAAAACGAAGTTCATAATAGATGAAATTACTCACCAATTAAT
CGAAAATCCATCTGGAAAGCCGATTATTTATATAGTCCCTGATCAAATGACTTTTTTATCCGAATATACGCTTGTGAATA
CTCCGGGATTGAACGGCATGATGCGTGCTCAAGTTTATAGTTTTTCGCGTCTTGCATGGAAAGTTCTTCAAGAAACGGGA
GGATTCGCTCGGATTCACTTGTCTTCAGCCGGTTTAAATATGCTGATTCGAAAAATCATCGAAGAAAGAAAAGAGAATCT
GCGGCTTTTTGCTAAAGCAGCAGACAAATCGGGGTTTATTCAGCATGTGGAAAAAGCGCTTATTGAACTGAAACGCTATT
GCGTTGGCACTGATGAACTTGCGGCCAGGAGAACGGAGTTTGAGGAAAATGGAGAGGCCCAGCTTTTGATTGATAAATTG
CACGATCTCCACTTGATTTACGAAGAGTTTGAAAATCATCTCGTTGACCGCTATATTGCATCGGAGGATTATTTTCAATT
ATTGGCGGAGTCCATCCAAAAGTCGGAGTATTTGCAAGACGCTCAAATTTATATCGATGGATTCCACAGTTTTACTCCCC
AAGAATACTTGGTGATCTACGAATTGATGAAAGCTGCATCTCAAGTTTCGATTGCGCTGACACTGGACCGTCCTTATTCA
GAAGAACTTCCGCGAGAAACGGATTTGTTCCGGATGACTGGCGAAACTTATGCTTCCTTGTGGAAAATGGCCCGCGATTC
CGGGATATCGACAGAAAATATCATGATGACGGAGTTTGTTCGCAGCAAGGAACAGAGTCTTATTCATTTGGAACGCTTTT
TTGACAAAAGGCCGGCCAAGCCGTTTATTGGGGAAGGCGCGCCCGTTGTTTTCGAGGCGGCGAACAGGAGAGCAGAGATT
GAAGGAGTGGCAAGAGAAATCCGAAGACTTGTCCGTGAACATCATCATCGTTATAAAGAGATAGCAGTGTTGGTCAGAAA
CGGACAAGATTATCAGGAATTGATTGAAACTATTTTTTATGACTACGAGATTCCGTTTTTTATTGATCAAAAAAGAACCA
TGTTAAATCATCCTTTTATTGAATTGATTCGTTCCAGTCTTGAAATTTTAACTAGCTTTTGGCGTTATGAACCGGTTTTT
CGCGCTGTCAAAACAGAGCTTCTTTATCCTTTCAAGATGAAAGCAGAGCAGATAAGAGAAAAAATAGACCGGTTTGAAAA
CTATTGTTTGGCTCACGGAGTGAAAGGGGAACTGTGGACAACAAAAAAACGCTGGGTTTACAGAAGATACAGAGGACTTG
AGCTGACGGACGCACCGCAAACGGACGAGGAGCGGGCAATAGAGGAAGAAATAAACGAATTGCGGGCTATGATTCGAACG
CCTGTTTTACGGCTCGATCGACGGTTTCGTCAAGCGAAAAACGGAAGAGATTTTTGCGAGGCGCTTTATAAGTATTTTGA
AGAACTGGATATTCCGGCGAAACTGGAAAGACTCAGTATGGAAGCTGAAGAAAACGGGGATTTACTGGCAGCTCGTGATC
ATGAACAAGCGTGGAATGCTATCATTGGATTGCTGGACGAGTTTGTAGAGATGCTTGGCGACCAGCCTTTTACGCTGAAG
AAATTCGCAACGATCCTCGAAGCGGGCATTGAATCGATGCGATTTACCAATGTTCCACCATCTGCCGATCAAGTCATCAT
CGCCGATTTGGAATTGTCACGTCTTTCGGATATCAAGACCGCATTTATCGTCGGAGTGAACGATGGAGTTTTGCCGGCCA
AAATGGCAGAGGAAGGTATTTTGACGGATGAAGAGAGGGAACGGATGGCTTCATTAGGGATGAAGCTTGCACCAACGAGC
AAAACCAGGTTGCTGAATGAAGAGTTTATTGCTTACAAAGCGTTTACAACTCCATCTGAACAACTTTATCTATCTTACCC
GATCGCTAATGAAGAAGGGAAAGCTTTGCTTCCTTCCCTCTACATCAAAAGAATGAAGGAAATATATCCGAATATAAAAG
AACAGCTATTGTTGAACGATCCTTCCGAACTGGAGGAGCAGGATCAACTGTCTTACATTTCCCATCCGGGGACGGCTATT
TCGTATTTAACGTCTCAAATTCAGCTTAAATTACGGAATTATCCAATGTATTCCATTTGGTGGGATGTCTACAATTTTTA
TATGAGCCACCAAGATTGGAAGTGGAAGGCAAGGAGAATTTTGTCCAGTTTGTTTTATCGAAATGAAGCGAAGAATTTAA
CAGAGGAAACAAGCAAAGAGCTTTACGGAGACCATATTTTGGCTAGCGTTTCGAGAATGGAGCTTTTCCATGGCTGTCCG
TTTTCCCATTTTGCTCGATTCGGGCTCGGTCTTCGAGAACGAGAGGTGTTCCGTTTAGAAGCTCCCGATATCGGTGAGCT
GTTCCACGGAGCGTTAAAATGGATGGCCGATGAAGTGAAAAAAAGAGGTCTCGGCTGGAAGACATTGACGAAAGATCAGT
GTGCGCAATTGGCAAGAGAATCGATTCAATATTTGGCGCCAAAGCTGCAAAATCAAATTTTATTGAGTTCGAACCGCTAT
CATTATATTAAGAAAAAGCTTGAATCTATTATCAGCAAGGCTTCTTACATTTTAAGCGAACATTCACGGGCAAGCGGGTT
TGAGCCAATCGGGCTGGAATTAGGATTTGGACCAAAAGCAGAACTTCCGCCTTTTACGTTCACTTTGAAAAACGGCATCA
AAATGGAGCTGGCAGGAAGAATCGATCGTGTTGATAAAGCGGAAGAAAACAGTAGTGTATTTTTAAGGGTGATTGATTAT
AAATCCAGCAGCAAGGATCTTGATTTAACAGAAGTTTATTATGGTCTTGCTCTTCAAATGTTGACGTATTTAGATATTGT
GTTGGCGAATTCGGAACAGTTGATAGGAACCCCTGCATTGCCTGCCGGAGTGTTGTATTTCCATGTCCATAATCCGATGC
TGAAAACGAATAAAATGCTGACGATGGACGAGGTGGAAGAAGAAATTTTTAAACAATACAAAATGAAAGGACTTGTGCTA
AGCGATCCGAACGTGGTCCGATTGATGGATCAAACTTTGGAAACGGGGGATTCGGCCATCATTTCCGCCGGACTGAAAAA
AGACGGAACGCTTAACGCGCGTTCAAAAGCAGCTTCTGATAATGATTTTGAATCGCTGAGAAAATATGTACGGAAGTTCT
ATGAAAAGACCGGCAATGATATTGTCTCCGGGAAAATCGATATTGCCCCATACAAATATAAAAAGAAAACACCTTGCGAA
TTTTGTTCTTTCCGTTCACTGTGCCAGTTTGACCCAAGTTTTGAGGAAAATCGATATCGAATCATCATACCTCTAAAAGA
TGAAGATGTATTAAAGTTGATACGCGAAAGGGGCGAGCAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(304-381)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(788-1129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

50.3

100

0.505

Multiple sequence alignment