Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BSM4216_RS00510 Genome accession   NZ_CP012024
Coordinates   108742..110115 (+) Length   457 a.a.
NCBI ID   WP_048622358.1    Uniprot ID   -
Organism   Bacillus smithii strain DSM 4216     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 103742..115115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSM4216_RS00490 (BSM4216_0105) - 103835..104296 (+) 462 WP_003354819.1 CtsR family transcriptional regulator -
  BSM4216_RS00495 (BSM4216_0106) - 104313..104867 (+) 555 WP_003354818.1 UvrB/UvrC motif-containing protein -
  BSM4216_RS00500 (BSM4216_0107) - 104857..105927 (+) 1071 WP_048622356.1 protein arginine kinase -
  BSM4216_RS00505 (BSM4216_0108) clpC 105943..108387 (+) 2445 WP_048622357.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BSM4216_RS00510 (BSM4216_0109) radA 108742..110115 (+) 1374 WP_048622358.1 DNA repair protein RadA Machinery gene
  BSM4216_RS00515 (BSM4216_0110) disA 110119..111186 (+) 1068 WP_003354814.1 DNA integrity scanning diadenylate cyclase DisA -
  BSM4216_RS00520 (BSM4216_0111) - 111387..112472 (+) 1086 WP_048624343.1 PIN/TRAM domain-containing protein -
  BSM4216_RS00525 (BSM4216_0112) ispD 112495..113184 (+) 690 WP_048622359.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BSM4216_RS00530 (BSM4216_0113) ispF 113203..113685 (+) 483 WP_048622360.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50152.58 Da        Isoelectric Point: 6.7690

>NTDB_id=150487 BSM4216_RS00510 WP_048622358.1 108742..110115(+) (radA) [Bacillus smithii strain DSM 4216]
MVKKKTKFICQSCGYESPKWMGRCPGCGEWNQMVEEVDMTGRQKKTFIHSESSRISKPSPLITVQSTEEPRTTTNMKEFN
RVLGGGIVAGSLVLIGGDPGIGKSTLLLQISSQLASGGHKVLYISGEESAKQIKLRADRLNVDSSNLYIYSETNLELINL
AIDQLNPEFVIIDSIQTVYHPEVTSAPGSVSQVRESTAEFMRMAKVQGMAIFIVGHVTKEGSIAGPRILEHMVDTVLYFE
GERHHSYRILRAVKNRFGSTNEMGIFEMKETGLIEVENPSEIFLEERAQSASGSTVVASMEGTRPVLVEIQALIAPTSFN
YPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIVSSFRDKPTRSTDCIIGEVGLTG
EVRRVTRIEQRVREAEKLGFSRVIIPAHNLGGWNTPGKIEVIGVSTVEEALEYALGG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=150487 BSM4216_RS00510 WP_048622358.1 108742..110115(+) (radA) [Bacillus smithii strain DSM 4216]
ATGGTAAAGAAAAAAACAAAATTTATTTGTCAATCATGCGGATATGAATCGCCGAAATGGATGGGGAGATGTCCCGGATG
CGGGGAATGGAACCAAATGGTGGAAGAAGTGGATATGACGGGGCGACAAAAGAAAACATTTATTCATTCCGAATCTTCGC
GGATTTCGAAGCCATCACCGCTCATTACAGTACAATCAACGGAAGAGCCTAGAACGACAACCAATATGAAAGAATTCAAC
CGTGTTTTAGGCGGAGGAATCGTCGCCGGCTCTCTTGTTTTAATCGGAGGAGATCCGGGTATCGGAAAGTCGACATTGTT
GCTGCAAATTTCCAGCCAGCTAGCGAGCGGGGGCCATAAAGTTTTATATATATCCGGAGAAGAATCGGCGAAACAAATCA
AACTGCGTGCGGATCGCTTGAATGTGGATTCTAGCAATCTCTATATCTATTCAGAAACCAATTTGGAATTAATAAACCTT
GCGATCGATCAATTAAATCCGGAATTTGTCATCATTGATTCGATTCAAACTGTCTACCACCCTGAAGTTACTTCCGCCCC
GGGCAGCGTTTCCCAAGTGCGGGAAAGTACGGCCGAATTCATGAGAATGGCGAAGGTCCAAGGAATGGCTATCTTTATTG
TAGGGCACGTAACGAAAGAAGGCTCGATTGCCGGTCCTAGAATTTTGGAGCATATGGTTGATACAGTTCTTTATTTCGAA
GGGGAGCGGCATCATTCTTACCGCATTTTAAGAGCGGTTAAAAATCGCTTTGGTTCTACAAATGAGATGGGAATTTTTGA
AATGAAAGAAACCGGACTCATAGAGGTGGAAAATCCGTCGGAAATCTTTTTGGAGGAGCGTGCGCAAAGTGCATCGGGGT
CAACGGTAGTTGCTTCTATGGAAGGGACACGCCCCGTGTTGGTTGAGATACAGGCATTGATAGCACCGACCAGTTTTAAT
TATCCGCGTCGAATGGCCACAGGGATCGATCATAATAGAGTGTCTCTTTTAATGGCGGTATTGGAAAAAAGGGTCGGCCT
TCTTCTTCAAAATCAAGATGCTTATTTAAAAGTGGCTGGGGGAGTCAAGCTTGATGAACCTGCCATTGATTTAGCGGTTG
CTGTCAGCATTGTGTCCAGTTTCCGCGACAAGCCGACACGATCAACAGATTGTATAATTGGAGAAGTAGGATTAACCGGT
GAAGTCAGAAGGGTAACGAGAATTGAACAGCGAGTGCGAGAAGCAGAAAAGCTGGGATTTTCCCGTGTCATTATTCCGGC
CCATAATCTTGGTGGTTGGAATACTCCAGGGAAAATAGAGGTGATTGGAGTTTCAACAGTAGAAGAAGCATTGGAGTATG
CGTTGGGAGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(75-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.331

100

0.726
  radA Streptococcus mitis NCTC 12261

62.832

98.906

0.621
  radA Streptococcus pneumoniae Rx1

62.832

98.906

0.621
  radA Streptococcus pneumoniae D39

62.832

98.906

0.621
  radA Streptococcus pneumoniae R6

62.832

98.906

0.621
  radA Streptococcus pneumoniae TIGR4

62.832

98.906

0.621
  radA Streptococcus mitis SK321

62.832

98.906

0.621

Multiple sequence alignment