Detailed information NTG0150418

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACJ67_RS04980
• Genome accession: NZ_CP012020
• Coordinates: 1032639..1033511 (+)
• Length: 290 a.a.
• NCBI ID: WP_049639782.1
• Uniprot ID: A0A0K0SZ43
• Organism: Methylophilus sp. TWE2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1027639..1038511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJ67_RS04955 (ACJ67_04955)	-	1029171..1030400 (+)	1230	WP_049638124.1	aspartate kinase	-
ACJ67_RS04970 (ACJ67_04970)	-	1031073..1032002 (+)	930	WP_049638125.1	polyprenyl synthetase family protein	-
ACJ67_RS04975 (ACJ67_04975)	-	1032045..1032440 (-)	396	WP_049638126.1	hypothetical protein	-
ACJ67_RS04980 (ACJ67_04980)	pilD	1032639..1033511 (+)	873	WP_049639782.1	A24 family peptidase	Machinery gene
ACJ67_RS04985 (ACJ67_04985)	coaE	1033508..1034110 (+)	603	WP_049638127.1	dephospho-CoA kinase	-
ACJ67_RS04990 (ACJ67_04990)	zapD	1034180..1034953 (+)	774	WP_049638128.1	cell division protein ZapD	-
ACJ67_RS14600	-	1034950..1035141 (+)	192	WP_197080668.1	DNA gyrase inhibitor YacG	-
ACJ67_RS04995 (ACJ67_04995)	-	1035163..1035606 (+)	444	WP_049638129.1	copper chaperone PCu(A)C	-
ACJ67_RS05000 (ACJ67_05000)	-	1035645..1035872 (-)	228	WP_049638130.1	VF530 family DNA-binding protein	-
ACJ67_RS05005 (ACJ67_05005)	-	1035898..1036851 (-)	954	WP_049638131.1	Nudix family hydrolase	-
ACJ67_RS05010 (ACJ67_05010)	-	1036857..1037714 (-)	858	WP_049638132.1	ATP-binding protein	-
ACJ67_RS05015 (ACJ67_05015)	-	1037873..1038445 (+)	573	WP_049638133.1	nucleoside triphosphate pyrophosphatase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31747.02 Da
• Isoelectric Point: 8.2202

>NTDB_id=150418 ACJ67_RS04980 WP_049639782.1 1032639..1033511(+) (pilD) [Methylophilus sp. TWE2]
MSEILEPVLSLLRTQPHFLQAVCLVLGLVVGSFLNVVIHRLPKMMEREWQASCLDLQGQTAPELPKYNLVIPRSACPHCG
HQITALENIPVISYLFLRGRCKACKAPISARYPMVELLTGLLSLAVGVQYGFSMLTVFALVFVFALVALTFIDFDTQLLP
DDITLPLLWLGLLLNTKYGFTDLTSAVIGAMAGYLVLWSVYWLFKLITGKEGMGYGDFKLLAAIGAWFGWQLLPAVILLS
SVVGSMIGIGLILIKGKTRQTAIPFGPFLALGGITALFYGQSLASYYLVP

Nucleotide

• Length: 873 bp

>NTDB_id=150418 ACJ67_RS04980 WP_049639782.1 1032639..1033511(+) (pilD) [Methylophilus sp. TWE2]
ATGTCAGAAATCCTTGAGCCTGTTTTAAGCTTATTACGTACCCAGCCGCATTTCTTGCAAGCGGTGTGTTTAGTGCTCGG
TTTAGTGGTGGGCAGTTTTCTCAATGTGGTCATACATCGCCTGCCAAAAATGATGGAGCGGGAGTGGCAGGCCAGTTGCC
TGGACTTGCAGGGCCAAACCGCGCCTGAGCTCCCGAAATATAACCTCGTTATACCGCGTTCTGCCTGCCCACATTGTGGA
CACCAGATTACCGCACTAGAAAATATTCCTGTCATCAGTTACCTGTTTTTGCGTGGCCGCTGCAAGGCATGCAAGGCGCC
AATTTCTGCCCGTTATCCAATGGTGGAGTTGTTGACGGGTTTGTTATCACTGGCGGTTGGTGTGCAATATGGTTTTAGCA
TGTTGACCGTATTTGCTTTGGTGTTTGTATTTGCGCTGGTTGCATTGACTTTTATCGATTTTGATACCCAATTATTACCC
GATGATATAACGCTACCCTTGTTGTGGCTGGGTCTGTTATTGAATACCAAGTATGGTTTCACAGATTTAACTTCAGCCGT
GATTGGTGCCATGGCGGGTTACCTGGTGTTATGGTCTGTCTACTGGTTGTTCAAACTGATCACAGGTAAAGAAGGCATGG
GTTATGGCGACTTTAAGCTCCTGGCTGCGATTGGTGCCTGGTTTGGCTGGCAGTTATTACCTGCAGTCATCTTGTTGAGT
TCAGTGGTTGGCAGCATGATCGGCATAGGCCTGATTCTGATCAAAGGTAAAACTCGCCAAACGGCTATTCCCTTTGGCCC
CTTTCTGGCCTTAGGTGGGATTACTGCCTTATTTTACGGTCAGTCACTGGCTTCTTATTATCTCGTGCCATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-249)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-129)

3D structure

• AlphaFold DB: A0A0K0SZ43

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.737	98.276	0.538
pilD	Vibrio campbellii strain DS40M4	51.59	97.586	0.503
pilD	Acinetobacter baumannii D1279779	52.222	93.103	0.486
pilD	Acinetobacter nosocomialis M2	51.852	93.103	0.483
pilD	Neisseria gonorrhoeae MS11	51.19	86.897	0.445