Detailed information NTG0150186

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: SAHC1340_RS09325
• Genome accession: NZ_CP012011
• Coordinates: 1829383..1830069 (-)
• Length: 228 a.a.
• NCBI ID: WP_001792568.1
• Uniprot ID: A0A133PXY3
• Organism: Staphylococcus aureus strain HC1340
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1824383..1835069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAHC1340_RS09305 (SAHC1340_02525)	rpsT	1825297..1825548 (+)	252	WP_001274017.1	30S ribosomal protein S20	-
SAHC1340_RS09310 (SAHC1340_02526)	holA	1825593..1826567 (-)	975	WP_001282562.1	DNA polymerase III subunit delta	-
SAHC1340_RS09315 (SAHC1340_02527)	comEC	1826624..1828825 (-)	2202	WP_001807336.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SAHC1340_RS09320 (SAHC1340_02528)	-	1828830..1829291 (-)	462	WP_000439693.1	ComE operon protein 2	-
SAHC1340_RS09325 (SAHC1340_02529)	comEA	1829383..1830069 (-)	687	WP_001792568.1	ComEA family DNA-binding protein	Machinery gene
SAHC1340_RS09330 (SAHC1340_03324)	-	1830109..1830824 (-)	716	Protein_1811	class I SAM-dependent DNA methyltransferase	-
SAHC1340_RS09335 (SAHC1340_02532)	rsfS	1830827..1831180 (-)	354	WP_001088022.1	ribosome silencing factor	-
SAHC1340_RS09340 (SAHC1340_02533)	yqeK	1831181..1831765 (-)	585	WP_001019324.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SAHC1340_RS09345 (SAHC1340_02534)	nadD	1831755..1832324 (-)	570	WP_000725162.1	nicotinate (nicotinamide) nucleotide adenylyltransferase	-
SAHC1340_RS09350 (SAHC1340_02535)	yhbY	1832327..1832617 (-)	291	WP_000955231.1	ribosome assembly RNA-binding protein YhbY	-
SAHC1340_RS09355 (SAHC1340_02536)	aroE	1832621..1833427 (-)	807	WP_000666761.1	shikimate dehydrogenase	-
SAHC1340_RS09360 (SAHC1340_02537)	yqeH	1833441..1834541 (-)	1101	WP_001280141.1	ribosome biogenesis GTPase YqeH	-
SAHC1340_RS09365 (SAHC1340_02538)	-	1834542..1835069 (-)	528	WP_000524902.1	YqeG family HAD IIIA-type phosphatase	-

Sequence

Protein

• Length: 228 a.a.
• Molecular weight: 25863.49 Da
• Isoelectric Point: 9.3305

>NTDB_id=150186 SAHC1340_RS09325 WP_001792568.1 1829383..1830069(-) (comEA) [Staphylococcus aureus strain HC1340]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide

• Length: 687 bp

>NTDB_id=150186 SAHC1340_RS09325 WP_001792568.1 1829383..1830069(-) (comEA) [Staphylococcus aureus strain HC1340]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (164-227)

3D structure

• AlphaFold DB: A0A133PXY3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Staphylococcus aureus MW2	98.238	99.561	0.978
comEA	Staphylococcus aureus N315	97.357	99.561	0.969