Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ABU16_RS10575 Genome accession   NZ_CP011882
Coordinates   2050276..2053974 (+) Length   1232 a.a.
NCBI ID   WP_032730448.1    Uniprot ID   -
Organism   Bacillus subtilis strain TO-A JPC     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2045276..2058974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU16_RS21760 yhjQ 2045724..2046050 (-) 327 WP_009966950.1 four-helix bundle copper-binding protein -
  ABU16_RS10565 (ABU16_2072) yhjR 2046168..2046605 (-) 438 WP_003245806.1 ferritin family protein -
  ABU16_RS10570 (ABU16_2073) addB 2046789..2050289 (+) 3501 WP_003244988.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ABU16_RS10575 (ABU16_2074) addA 2050276..2053974 (+) 3699 WP_032730448.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ABU16_RS10580 (ABU16_2075) sbcD 2054046..2055221 (+) 1176 WP_032721297.1 exonuclease subunit SbcD -
  ABU16_RS10585 (ABU16_2076) sbcC 2055218..2058610 (+) 3393 WP_048217633.1 exonuclease subunit SbcC -
  ABU16_RS10590 (ABU16_2077) hlpB 2058624..2058926 (+) 303 WP_015252406.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1232 a.a.        Molecular weight: 141008.30 Da        Isoelectric Point: 5.0430

>NTDB_id=149177 ABU16_RS10575 WP_032730448.1 2050276..2053974(+) (addA) [Bacillus subtilis strain TO-A JPC]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPGAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFSELYKRVPVVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLAEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAENDKGECEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEDTDASEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLNTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEDLAGVPAHADISDHPARFAVQMIHSYDLL
DDDLEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPADG
SILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVHRLYEKELLTEEQKDAIDIEEIVQFFHTEIGGQL
IGAKWKDREIPFSLALPAKEIYPDAQVADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYETQ
IQLYTKAVEQIAKTKVKGCALYFFDGGHSLTL

Nucleotide


Download         Length: 3699 bp        

>NTDB_id=149177 ABU16_RS10575 WP_032730448.1 2050276..2053974(+) (addA) [Bacillus subtilis strain TO-A JPC]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CAATAGATGTAGACCGTCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAGCACCGAATCGCAGAAGCC
TTGGAAAAAGAGCTTGTACAGCGCCCCGGCTCGCTGCATATTAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACGCTCCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTCGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATACGCAAAGGGTGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTATACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTTAAACAGGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGGGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAAAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAAGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTCGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCAGTGAACTATATAAGCGGGTGCCCGTCGTCTCTTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCAGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCAAAAAAACTGCTTGAAAAGCTCAAA
ACCGACTACTTCACGCGAAGTCCTGAACAGCACTTGAAAAGCCTAGCCGAGATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGATTCGAAGCTGCGAAACAGGAGAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTGAGAATGACAAAGGTGAATGTGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAACAG
TTTCATGAAGTGCTCGTTGACGAATATCAGGATACCAACCTCGTGCAGGAATCGATTCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCTAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGGCGGAAAATCGATTTAAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCAGCGTATCCGGACAATGACGAGACGGAAACAGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAGCGAGGAAGCAGAAGAGCTTGAAACGGTGCAGTTTGAGGCCAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACATCGCAATATTCAATACCGGGATATCGTGAT
TTTGCTCCGCTCCATGCCTTGGGCTCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTGA
CGTCAGGCTATTTCGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTAATCGATAATCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAGGCGATGAAAGACTACCTGGCTGCTGGTGACCGGAACGATGAGCTTTATCAAAAGCTAAATACGT
TTTACGGACATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACCAAATATATGGATTATGTCGGCGGCATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGTCAATATGAATCAACGGCATTTCGCGGCTTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGA
GCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGACACTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCATGTAAGGATCATCAGAAACAGCTTGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTTCTGCCAGA
ATTTGACCGCTATCAGGCGAAAACGTATCTAGATTTCATCGGGCCGGCTCTTGCCAGGCACAGAGACTTGGAGGATTTGG
CTGGTGTGCCAGCACACGCTGACATCTCAGATCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCTATGATTTGCTT
GATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCCCGGCTC
GTTTGCGTTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCGCATCACGAGGTGACGCAAATTCGGACAAAGC
AATCGGTTTCTGAGATCAAAAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCTGTAAAGCCGGCTGATGGA
AGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGGACTGCCATGCATACGGT
TATGCAGCATATCCCGCTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCACAGGCTTTATGAAAAAGAGC
TTCTCACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGACAGCTG
ATCGGCGCAAAGTGGAAGGACAGGGAAATACCATTCAGCTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGCACAGGT
GGCAGATGAGCCGCTTTTAGTGCAGGGTATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATCTATTGGATTATA
AGTCGGACCGGATTGAGGGCAAATTCCAGCATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAAACGCAA
ATTCAGCTGTACACGAAGGCAGTGGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGACGGAGG
GCACAGTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1053-1223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

98.864

100

0.989

Multiple sequence alignment