Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   ACG10_RS16355 Genome accession   NZ_CP011835
Coordinates   3518379..3519977 (+) Length   532 a.a.
NCBI ID   WP_089169922.1    Uniprot ID   -
Organism   Azotobacter chroococcum strain B3     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3513379..3524977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG10_RS16335 (ACG10_15970) - 3513490..3514506 (-) 1017 WP_232487129.1 substrate-binding domain-containing protein -
  ACG10_RS16340 (ACG10_15975) - 3514970..3515287 (+) 318 WP_089169017.1 hypothetical protein -
  ACG10_RS23835 - 3515334..3515660 (-) 327 WP_157731474.1 hypothetical protein -
  ACG10_RS16345 (ACG10_15980) - 3516250..3517884 (-) 1635 WP_198318963.1 NAD+ synthase -
  ACG10_RS16350 (ACG10_15985) - 3518111..3518353 (+) 243 WP_089169019.1 PP0621 family protein -
  ACG10_RS16355 (ACG10_15990) pilS 3518379..3519977 (+) 1599 WP_089169922.1 PAS domain-containing sensor histidine kinase Regulator
  ACG10_RS16360 (ACG10_15995) pilR 3520021..3521361 (+) 1341 WP_089169020.1 sigma-54 dependent transcriptional regulator Regulator
  ACG10_RS16365 (ACG10_16000) thiO 3521496..3522593 (-) 1098 WP_089169021.1 glycine oxidase ThiO -
  ACG10_RS16370 (ACG10_16005) - 3522773..3523336 (+) 564 WP_157731475.1 GspH/FimT family pseudopilin -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58738.98 Da        Isoelectric Point: 8.4900

>NTDB_id=148926 ACG10_RS16355 WP_089169922.1 3518379..3519977(+) (pilS) [Azotobacter chroococcum strain B3]
MIGTPLDGPQGGNVLRLYHLYRLVIGLLLVLLISSNLDERLLQVAHPELFHHGSWFYLILNILVAVLVHHPKPLQIFSLA
LVDVILLSGLLYTAGGPSSGIGNLIIVSVAVANILLHGRIGLLIAAVAASGLVYMTFYLGLNRPAAASQYVHAGALGSLC
FASALLVQSLTRRLRRSESLAERRAAEVADLQALNVQILERMRTGILVLDPQQQVLLANQSAFTLLGNPALTGQPLAAHC
PELVERLQQWQQNPTLRPQDLHTLPHGPLVQPNFVALTRGGRRGILVFLEDISQIAQQAQQLKLASLGRLTAGIAHEIRN
PLGAISHAAQLLQESECLDGPDRRLAQIIQDQSRRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFAGEYRASAAPNQIL
HLEIRDGSLKTRMDANQLIQILTNLVQNGLRYSAQCHPQGQVWLRLFRAPESDLPVLEVRDDGPGVPAEQIEHIFEPFFT
TETKGTGLGLYISRELCESNQARLDYRGGEGEGGCFRITFAHPRKLTQARHG

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=148926 ACG10_RS16355 WP_089169922.1 3518379..3519977(+) (pilS) [Azotobacter chroococcum strain B3]
CTGATCGGCACCCCGCTGGACGGCCCTCAGGGCGGCAACGTCCTGCGCCTGTACCACCTGTACCGGCTGGTCATCGGCCT
GCTGCTGGTGCTGCTGATCTCCAGCAATCTGGACGAGCGGCTGCTGCAGGTCGCCCACCCGGAGCTGTTCCACCACGGCA
GCTGGTTCTACCTGATCCTGAACATTCTGGTCGCCGTTCTAGTGCACCACCCCAAACCCCTGCAGATCTTCAGCCTGGCA
CTGGTCGACGTGATCCTGCTTTCGGGCCTGCTCTACACGGCCGGTGGCCCGTCCAGCGGCATCGGCAACCTGATCATCGT
CTCGGTGGCCGTCGCCAACATCCTCCTGCACGGACGGATCGGCCTGCTGATCGCCGCCGTGGCGGCCAGCGGCCTGGTCT
ACATGACCTTCTACCTGGGCCTCAACCGCCCTGCGGCCGCCAGCCAGTACGTGCATGCGGGAGCGCTGGGCTCGCTGTGC
TTCGCCAGCGCGCTGCTCGTGCAGAGCCTGACCCGCCGCCTGCGGCGCAGCGAATCCCTGGCCGAACGACGCGCCGCCGA
GGTCGCCGACCTACAGGCCCTGAATGTACAGATCCTGGAGCGCATGCGGACCGGCATCCTGGTGCTCGACCCGCAGCAGC
AGGTGCTGCTGGCCAATCAGAGCGCCTTCACCCTGCTGGGCAACCCCGCACTGACCGGACAGCCGCTCGCCGCCCACTGC
CCGGAGCTGGTCGAGCGCCTGCAGCAGTGGCAGCAGAACCCGACCCTGCGCCCGCAGGACCTGCACACCCTGCCACACGG
TCCGCTGGTGCAACCGAACTTCGTCGCCCTCACGCGGGGCGGACGGCGGGGCATCCTGGTCTTCCTCGAGGACATCTCGC
AGATCGCCCAGCAGGCCCAGCAGCTCAAGCTCGCCTCGCTCGGCCGGCTGACCGCCGGCATCGCCCACGAGATCCGCAAT
CCGCTCGGCGCCATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCTGAGTGCCTCGACGGCCCCGACCGGCGCCTGGCGCA
GATCATCCAGGACCAGTCGCGGCGCATGAACCTGGTGATCGAGAACGTGCTGCAGCTGTCGCGCCGGCGCCAGGCCGAAC
CGCAGCTGCTCGACCTGAAGTACTGGCTGCACCGCTTCGCCGGCGAATACCGCGCCTCGGCGGCGCCGAACCAGATCCTG
CACCTGGAGATCCGGGACGGCTCCCTGAAGACCCGCATGGACGCGAACCAGCTGATCCAGATCCTCACCAACCTAGTGCA
GAACGGCCTGCGCTACAGCGCCCAGTGCCACCCGCAGGGACAGGTCTGGCTCAGGCTGTTCCGCGCCCCGGAAAGCGACC
TGCCGGTCCTCGAAGTGCGCGATGACGGCCCCGGCGTGCCCGCCGAGCAGATCGAGCACATCTTCGAGCCCTTCTTCACC
ACCGAAACCAAGGGCACCGGGCTGGGCCTGTACATCTCCCGCGAGCTGTGCGAAAGCAACCAGGCCCGCCTCGACTACCG
CGGCGGCGAAGGTGAAGGCGGCTGCTTCCGCATCACCTTCGCCCACCCACGCAAGCTGACCCAGGCACGCCACGGATGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(414-520)

His Kinase A (phospho-acceptor) domain (HisKA)

(306-370)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

65.201

98.308

0.641

Multiple sequence alignment